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Ricin A chain bound to camelid nanobody (VHH4)

X-ray diffraction
2.7Å resolution
PDB entry 4lgs coloured by chain, front view.
PDB entry 4lgs coloured by chain, side view.
PDB entry 4lgs coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Ricin A chain and IG-heavy chain
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Multimeric state: hetero dimer
Accessible surface area: 17700.07 Å2
Buried surface area: 1106.01 Å2
Dissociation area: 553.01 Å2
Dissociation energy (ΔGdiss): -1.31 kcal/mol
Dissociation entropy (TΔSdiss): 11.61 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-208801

Macromolecules

Ricin A chain
Chain: A
Length: 263 amino acids
Theoretical weight: 29.43 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02879 (Residues: 39-301; Coverage: 49%)
Pfam: Ribosome inactivating protein
InterPro:
CATH:
Ricin A chain in PDB entry 4lgs, assembly 1, front view.
Ricin A chain in PDB entry 4lgs, assembly 1, side view.
Ricin A chain in PDB entry 4lgs, assembly 1, top view.
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Camelid nanobody (VHH4)
Chain: B
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli BL21(DE3)
InterPro:
CATH: Immunoglobulins
Camelid nanobody (VHH4) in PDB entry 4lgs, assembly 1, front view.
Camelid nanobody (VHH4) in PDB entry 4lgs, assembly 1, side view.
Camelid nanobody (VHH4) in PDB entry 4lgs, assembly 1, top view.
Molecule details ›

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