PDBe 4lgs

X-ray diffraction
2.7Å resolution

Function and Biology Details

Reaction catalysed:
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.43 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P02879 (Residues: 39-301; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:
Camelid nanobody (VHH4) Chain: B
Molecule details ›
Chain: B
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 91.391Å b: 49.935Å c: 114.442Å
α: 90° β: 103.88° γ: 90°
R R work R free
0.202 0.2 0.248
Expression system: Escherichia coli BL21(DE3)