› 4l6p

X-ray diffraction
2.68Å resolution

Structure of C22Y Mutant PCNA protein defective in DNA mismatch repair

Released:
DOI: 10.2210/pdb4l6p/pdb
PDB entry 4l6p coloured by chain, front view.
PDB entry 4l6p coloured by chain, side view.
PDB entry 4l6p coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Distinct structural alterations in proliferating cell nuclear antigen block DNA mismatch repair.
Dieckman LM, Boehm EM, Hingorani MM, Washington MT
Biochemistry 52 5611-9 (2013)
PMID: 23869605

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proliferating cell nuclear antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 264 amino acids
Theoretical weight: 29.83 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P15873 (Residues: 1-258; Coverage: 100%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 85.941Å b: 90.619Å c: 140.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.28
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Many Roles of PCNA in Eukaryotic DNA Replication.
Boehm et al. (2016)
12 other articles

1 mention without citation

Distinct structural alterations in proliferating cell nuclear antigen block DNA mismatch repair.
Dieckman et al. (2013)