PDBe 4gs7

X-ray diffraction
2.35Å resolution

Structure of the Interleukin-15 quaternary complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Interleukin-15 Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40933 (Residues: 49-162; Coverage: 86%)
Gene name: IL15
Structure domains: Growth Hormone; Chain: A;
Interleukin-2 receptor subunit beta Chain: B
Molecule details ›
Chain: B
Length: 217 amino acids
Theoretical weight: 25.15 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P14784 (Residues: 27-240; Coverage: 41%)
Gene names: IL15RB, IL2RB
Structure domains: Immunoglobulins
Soluble interleukin-15 receptor subunit alpha Chain: D
Molecule details ›
Chain: D
Length: 69 amino acids
Theoretical weight: 7.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13261 (Residues: 30-97; Coverage: 28%)
Gene name: IL15RA
Structure domains: Rubrerythrin, domain 2
Cytokine receptor common subunit gamma Chain: C
Molecule details ›
Chain: C
Length: 203 amino acids
Theoretical weight: 24.39 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P31785 (Residues: 55-254; Coverage: 58%)
  • Best match: P31785-2 (Residues: 9-64)
Gene name: IL2RG
Sequence domains: Interleukin-6 receptor alpha chain, binding
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 70.949Å b: 74.61Å c: 129.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.227
Expression systems:
  • Escherichia coli
  • Trichoplusia ni