2pm7

X-ray diffraction
2.35Å resolution

Crystal structure of yeast Sec13/31 edge element of the COPII vesicular coat, selenomethionine version

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and organization of coat proteins in the COPII cage.
Cell 129 1325-36 (2007)
PMID: 17604721

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-153785 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein transport protein SEC31 Chains: A, C
Molecule details ›
Chains: A, C
Length: 399 amino acids
Theoretical weight: 45.13 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Hi5 insect cells
UniProt:
  • Canonical: P38968 (Residues: 370-763; Coverage: 31%)
Gene names: D1229, SEC31, WEB1, YDL195W
Sequence domains: Sec23-binding domain of Sec16
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Protein transport protein SEC13 Chains: B, D
Molecule details ›
Chains: B, D
Length: 297 amino acids
Theoretical weight: 33.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Hi5 insect cells
UniProt:
  • Canonical: Q04491 (Residues: 1-297; Coverage: 100%)
Gene names: ANU3, L8167.4, SEC13, YLR208W
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 128.19Å b: 52.5Å c: 133.09Å
α: 90° β: 108.34° γ: 90°
R-values:
R R work R free
0.245 0.242 0.298
Expression system: Hi5 insect cells