Function and Biology

THE CRYSTAL STRUCTURE OF ANTHRANILATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS

Source organism: Sulfolobus solfataricus
Biochemical function: lyase activity
Biological process: cellular amino acid biosynthetic process
Cellular component: anthranilate synthase complex

EC 4.1.3.27: Anthranilate synthase

Reaction catalysed:
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Comments:
  • In some organisms, this enzyme is part of a multifunctional protein together with one or more components of the system for biosynthesis of tryptophan (EC 2.4.2.18, EC 4.1.1.48, EC 4.2.1.20 and EC 5.3.1.24).
  • The native enzyme in the complex with uses either glutamine or (less efficiently) NH(3).
  • The enzyme separated from the complex uses NH(3) only.
Systematic name:
Chorismate pyruvate-lyase (amino-accepting; anthranilate-forming)
Alternative Name(s):
  • Anthranilate synthetase
  • Chorismate lyase
  • Chorismate pyruvate-lyase (amino-accepting)
  • TrpE

Sequence families

Pfam Protein families (Pfam)
PF00117
Domain description: Glutamine amidotransferase class-I
Occurring in:
  1. Anthranilate synthase component 2
1 copy of Pfam domain PF00117 (Glutamine amidotransferase class-I) in Anthranilate synthase component 2 in PDB 1qdl.

PF04715
Domain description: Anthranilate synthase component I, N terminal region
Occurring in:
  1. Anthranilate synthase component 1
1 copy of Pfam domain PF04715 (Anthranilate synthase component I, N terminal region) in Anthranilate synthase component 1 in PDB 1qdl.

PF00425
Domain description: chorismate binding enzyme
Occurring in:
  1. Anthranilate synthase component 1
1 copy of Pfam domain PF00425 (chorismate binding enzyme) in Anthranilate synthase component 1 in PDB 1qdl.

InterPro InterPro annotations
IPR019999
Domain description: Anthranilate synthase component I-like
Occurring in:
  1. Anthranilate synthase component 1
IPR029062
Domain description: Class I glutamine amidotransferase-like
Occurring in:
  1. Anthranilate synthase component 2
IPR017926
Domain description: Glutamine amidotransferase
Occurring in:
  1. Anthranilate synthase component 2
IPR005801
Domain description: ADC synthase
Occurring in:
  1. Anthranilate synthase component 1
IPR006221
Domain description: Anthranilate synthase/para-aminobenzoate synthase like domain
Occurring in:
  1. Anthranilate synthase component 2
IPR015890
Domain description: Chorismate-utilising enzyme, C-terminal
Occurring in:
  1. Anthranilate synthase component 1
IPR006805
Domain description: Anthranilate synthase component I, N-terminal
Occurring in:
  1. Anthranilate synthase component 1
IPR010116
Domain description: Anthranilate synthase component I, archaeal type
Occurring in:
  1. Anthranilate synthase component 1

Structure domains

CATH CATH domains
3.60.120.10
Class: Alpha Beta
Architecture: 4-Layer Sandwich
Topology: Anthranilate synthase
Homology: Anthranilate synthase
Occurring in:
  1. Anthranilate synthase component 1
1 copy of CATH domain 3.60.120.10 (Anthranilate synthase) in Anthranilate synthase component 1 in PDB 1qdl.
3.40.50.880
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Rossmann fold
Occurring in:
  1. Anthranilate synthase component 2
1 copy of CATH domain 3.40.50.880 (Rossmann fold) in Anthranilate synthase component 2 in PDB 1qdl.
SCOP SCOP annotations
56323
Class: Alpha and beta proteins (a+b)
Fold: ADC synthase
Superfamily: ADC synthase
Occurring in:
  1. Anthranilate synthase component 1
1 copy of SCOP domain 56323 (ADC synthase) in Anthranilate synthase component 1 in PDB 1qdl.
52318
Class: Alpha and beta proteins (a/b)
Fold: Flavodoxin-like
Superfamily: Class I glutamine amidotransferase-like
Occurring in:
  1. Anthranilate synthase component 2
1 copy of SCOP domain 52318 (Class I glutamine amidotransferases (GAT)) in Anthranilate synthase component 2 in PDB 1qdl.