EMD-7963

Single-particle
3.9 Å
EMD-7963
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Deposition: 04/06/2018
Map released: 11/07/2018
Last modified: 29/07/2020
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7963
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6dmb summary report (pdf.gz) Map and 6dmb FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of human Ptch1

EMD-7963

Single-particle
3.9 Å
EMD-7963
3D View Gallery
Deposition: 04/06/2018
Map released: 11/07/2018
Last modified: 29/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 15 mg/mL
Buffer
pH: 8.0
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.9 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 94445
Software [1]
Name Version Details
RELION - -
⦨ Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION - -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION - -
CTF correction
Software [1]
Name Version Details
Gctf - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 224 224 224
Origin 0 0 0
Spacing 224 224 224
Voxel size 1.091 Å 1.091 Å 1.091 Å
Contour list
Primary Level Source
True 0.05 AUTHOR