EMD-7506
1.15 A MicroED structure of GSNQNNF at 1.8 e- / A^2
EMD-7506
Electron Crystallography1.15 Å
Deposition: 02/03/2018
Map released: 16/05/2018
Last modified: 13/03/2024
Concentration: 10
mg/mL
Buffer
Grid
Mesh: 300
Model: Quantifoil R2/2
Material: COPPER
Model: Quantifoil R2/2
Material: COPPER
Pretreatment
Support Film [1]
Material | Topology | Thickness |
---|---|---|
CARBON | HOLEY ARRAY | - |
Vitrification
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: DIFFRACTION
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Camera Length: 730 mm
Specimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: DIFFRACTION
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Camera Length: 730 mm
Specimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN
Image Recording
[1]
Detector model:
TVIPS TEMCAM-F416 (4k x 4k)
Dimensions: 2048 pixel x 2048 pixel
Number of grids: 1
Number of real images: 828
Number of diffraction images: 828
Average exposure time: 2.1 s
Average electron dose per image: 0.00357 e/Å2
Dimensions: 2048 pixel x 2048 pixel
Number of grids: 1
Number of real images: 828
Number of diffraction images: 828
Average exposure time: 2.1 s
Average electron dose per image: 0.00357 e/Å2
Final
reconstruction
Crystallography statistics
Number of intensities measured:
7517
Number of structure factors: 1287
Fourier space coverage: 79.7
R sym: 0.225
R merge: 0.225
Overall phase error: 44.13
Overall phase residual: 44.13
Phase error rejection criteria: 0
High resolution: 1.15 Å
Number of structure factors: 1287
Fourier space coverage: 79.7
R sym: 0.225
R merge: 0.225
Overall phase error: 44.13
Overall phase residual: 44.13
Phase error rejection criteria: 0
High resolution: 1.15 Å
Shell list
[1]
High resolution | Low resolution | Number of structure factors | Phase residual | Fourier space coverage | Multiplicity |
---|---|---|---|---|---|
1.15 Å | 1.18 Å | 115 | 66.98 | 83.94 | 6.2 |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: MicroED density map
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: MicroED density map
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 72 | 52 | 74 |
Origin | -7 | -31 | 20 |
Spacing | 74 | 72 | 52 |
Voxel size | 0.34643 Å | 0.36921 Å | 0.3975 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.32073 | AUTHOR |