EMD-5854
Cryo-electron tomography of Chlamydomonas axoneme, central pair apparatus (pf6)
EMD-5854
Subtomogram averaging72.0 Å
Deposition: 06/01/2014
Map released: 19/02/2014
Last modified: 07/05/2014
Concentration: 0.01
mg/mL
Buffer
pH: 7.2
Details: 30 mM Hepes-NaOH pH 7.2, 5 mM MgCl2, 1 mM dithiothreitol, 1 mM EGTA, and 50 mM NaCl
Details: 30 mM Hepes-NaOH pH 7.2, 5 mM MgCl2, 1 mM dithiothreitol, 1 mM EGTA, and 50 mM NaCl
Grid
Details: 300 mesh copper grid with home-made holey carbon support
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 93 K
Instrument: LEICA EM GP
Method: Blot for 5 seconds before plunging
Chamber humidity: 90%
Chamber temperature: 93 K
Instrument: LEICA EM GP
Method: Blot for 5 seconds before plunging
Microscope: JEOL 3100FFC
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.2 mm
Nominal magnification: 15000.0
Calibrated magnification: 25700.0
Specimen holder model: GATAN LIQUID NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.2 mm
Nominal magnification: 15000.0
Calibrated magnification: 25700.0
Specimen holder model: GATAN LIQUID NITROGEN
Temperature
Minimum: 90
K
Maximum: 95 K
Maximum: 95 K
Specialist optics
Energy filter
Image Recording
[1]
Detector category:
CCD
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Average electron dose per image: 90 e/Å2
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Average electron dose per image: 90 e/Å2
Tilt Series
[1]
Axis 1 | Axis 2 | |||||
---|---|---|---|---|---|---|
Min. | Max. | Inc. | Min. | Max. | Inc. | Rotation |
-60.5 ° | 60.5 ° | - | - | - | - | - |
Final
reconstruction
Resolution: 72.0
Å
(
BY AUTHOR)
Resolution method: OTHER
Resolution method: OTHER
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
PEET, RUBY-HELIX | - | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Averaged tomogram of axonemal central pair microtubules (pf6)
Details: ::::EMDATABANK.org::::EMD-5854::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Averaged tomogram of axonemal central pair microtubules (pf6)
Details: ::::EMDATABANK.org::::EMD-5854::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 180 | 180 | 180 |
Origin | 0 | 0 | 0 |
Spacing | 180 | 180 | 180 |
Voxel size | 6.0699997 Å | 6.0699997 Å | 6.0699997 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 34588.0 | AUTHOR |