3rtx

X-ray diffraction
2.81Å resolution

Crystal structure of mammalian capping enzyme (Mce1) and Pol II CTD complex

Released:
Source organism: Mus musculus
Primary publication:
Structural insights to how mammalian capping enzyme reads the CTD code.
Mol Cell 43 299-310 (2011)
PMID: 21683636

Function and Biology Details

Reactions catalysed:
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
hetero trimer
PDBe Complex ID:
PDB-CPX-129565 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
mRNA-capping enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 39.43 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O55236 (Residues: 226-567; Coverage: 57%)
Gene names: Cap1a, Rngtt
Sequence domains:
Structure domains:
RNA Polymerase II C-terminal domain Chain: C
Molecule details ›
Chain: C
Length: 18 amino acids
Theoretical weight: 2.23 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 82.98Å b: 114.75Å c: 150.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.28
Expression systems:
  • Escherichia coli
  • Not provided