› 1wvf

X-ray diffraction
1.3Å resolution

p-Cresol Methylhydroxylase: Alteration of the Structure of the Flavoprotein Subunit upon its Binding to the Cytochrome Subunit

Released:
DOI: 10.2210/pdb1wvf/pdb
PDB entry 1wvf coloured by chain, front view.
PDB entry 1wvf coloured by chain, side view.
PDB entry 1wvf coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
p-Cresol methylhydroxylase: alteration of the structure of the flavoprotein subunit upon its binding to the cytochrome subunit.
Cunane LM, Chen ZW, McIntire WS, Mathews FS
Biochemistry 44 2963-73 (2005)
PMID: 15723539

Function and Biology Details

Reaction catalysed: 
(1a) 4-methylphenol + 2 oxidized azurin + H(2)O = 4-hydroxybenzyl alcohol + 2 reduced azurin + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-cresol dehydrogenase [hydroxylating] flavoprotein subunit Chain: A
Molecule details ›
Chain: A
Length: 520 amino acids
Theoretical weight: 57.87 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P09788 (Residues: 2-521; Coverage: 100%)
Gene name: pchF
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand FAD 1 x FAD
Ligand ACY 2 x ACY
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 88.64Å b: 116.91Å c: 50.22Å
α: 90° β: 113.18° γ: 90°
R-values:
R R work R free
0.146 0.146 0.167
Expression system: Escherichia coli

Quick links

Citations

4 review citations

What's in a covalent bond? On the role and formation of covalently bound flavin cofactors.
Heuts et al. (2009)
3 more

2 mentions without citation

Oxidative Catabolism of (+)-Pinoresinol Is Initiated by an Unusual Flavocytochrome Encoded by Translationally Coupled Genes within a Cluster of (+)-Pinoresinol-Coinduced Genes in Pseudomonas sp. Strain SG-MS2.
Shettigar et al. (2020)
1 more