FAD-binding domain, PCMH-type (IPR016166)

Short name: FAD-bd_PCMH

Overlapping homologous superfamilies

Domain relationships


Flavoenzymes have the ability to catalyse a wide range of biochemical reactions. They are involved in the dehydrogenation of a variety of metabolites, in electron transfer from and to redox centres, in light emission, in the activation of oxygen for oxidation and hydroxylation reactions [PMID: 10694883]. About 1% of all eukaryotic and prokaryotic proteins are predicted to encode a flavin adenine dinucleotide (FAD)-binding domain [PMID: 16600599].

According to structural similarities and conserved sequence motifs,FAD-binding domains have been grouped in three main families: (i) theferredoxin reductase (FR)-type FAD-binding domain,(ii) the FAD-binding domains that adopt a Rossmann fold and (iii) the p-cresol methylhydroxylase (PCMH)-type FAD-binding domain [PMID: 11514662].

The FAD cofactor consists of adenosine monophosphate (AMP) linked to flavin mononucleotide (FMN) by a pyrophosphate bond. The AMP moiety is composed of the adenine ring bonded to a ribose that is linked to a phosphate group. The FMN moiety is composed of the isoalloxazine-flavin ring linked to a ribitol, which is connected to a phosphate group. The flavin functions mainly in a redox capacity, being able to take up two electrons from one substrate and release them two at a time to a substrate or coenzyme, or one at a time to an electron acceptor. The catalytic function of the FAD is concentrated in the isoalloxazine ring, whereas the ribityl phosphate and the AMP moiety mainly stabilise cofactor binding to protein residues [PMID: 10694883].

The PCMH-type FAD-binding domain consists of two alpha-beta subdomains: one is composed of three parallel beta-strands (B1-B3) surrounded by alpha-helices, and is packed against the second subdomain containing five antiparallel beta-strands (B4-B8) surrounded by alpha-helices [PMID: 10623531]. The two subdomains accommodate the FAD cofactor between them [PMID: 10694883]. In the PCMH proteins the coenzyme FAD is also covalently attached to a tyrosine located outside the FAD-binding domain in the C-terminal catalytic domain [PMID: 7248267].

This domain is found in:

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0071949 FAD binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE profiles