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CryoEM structure of Kappa Opioid Receptor bound to a semi-peptide and Gi1
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 8feg
Q-score: 0.471
Muratspahic E, Deibler K, Han J, Tomasevic N, Jadhav KB, Olive-Marti AL, Hochrainer N, Hellinger R, Koehbach J, Fay JF, Rahman MH, Hegazy L, Craven TW, Varga BR, Bhardwaj G, Appourchaux K, Majumdar S, Muttenthaler M, Hosseinzadeh P, Craik DJ, Spetea M, Che T, Baker D, Gruber CW
Nat Commun (2023) 14 pp. 8064- [ DOI: doi:10.1016/0006-2952(73)90196-2 Pubmed: 38052802 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 antibody fragment (26 kDa, Protein from Mus musculus)
- Ace-tyr-ala-dty-thr-thr-cys-thr-dpn-xt9 (1 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Kappa-type opioid receptor (34 kDa, Protein from Homo sapiens)
- Kappa opioid receptor in complex with gi1 heterotrimer and a peptide ligand (Complex from Homo sapiens)
momSalB bound Kappa Opioid Receptor in complex with Gi1
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 8dzp
Q-score: 0.533
Han J, Zhang J, Nazarova AL, Bernhard SM, Krumm BE, Zhao L, Lam JH, Rangari VA, Majumdar S, Nichols DE, Katritch V, Yuan P, Fay JF, Che T
Nature (2023) 617 pp. 417-425 [ DOI: doi:10.1038/s41586-023-06030-7 Pubmed: 37138078 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Kappa-type opioid receptor (34 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Momsalb bound kappa opioid receptor in complex with gi1 (Complex from Homo sapiens)
- Scfv16 protein (26 kDa, Protein from Mus musculus)
- Methyl (2s,4ar,6ar,7r,9s,10as,10br)-2-(furan-3-yl)-9-(methoxymethoxy)-6a,10b-dimethyl-4,10-dioxododecahydro-2h-naphtho[2,1-c]pyran-7-carboxylate (434 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
momSalB bound Kappa Opioid Receptor in complex with GoA
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 8dzq
Q-score: 0.459
Han J, Zhang J, Nazarova AL, Bernhard SM, Krumm BE, Zhao L, Lam JH, Rangari VA, Majumdar S, Nichols DE, Katritch V, Yuan P, Fay JF, Che T
Nature (2023) 617 pp. 417-425 [ DOI: doi:10.1038/s41586-023-06030-7 Pubmed: 37138078 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Kappa-type opioid receptor (34 kDa, Protein from Homo sapiens)
- Scfv16 protein (26 kDa, Protein from Mus musculus)
- Methyl (2s,4ar,6ar,7r,9s,10as,10br)-2-(furan-3-yl)-9-(methoxymethoxy)-6a,10b-dimethyl-4,10-dioxododecahydro-2h-naphtho[2,1-c]pyran-7-carboxylate (434 Da, Ligand)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Momsalb bound kappa opioid receptor in complex with goa (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
GR89,696 bound Kappa Opioid Receptor in complex with gustducin
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 8dzr
Q-score: 0.485
Han J, Zhang J, Nazarova AL, Bernhard SM, Krumm BE, Zhao L, Lam JH, Rangari VA, Majumdar S, Nichols DE, Katritch V, Yuan P, Fay JF, Che T
Nature (2023) 617 pp. 417-425 [ DOI: doi:10.1038/s41586-023-06030-7 Pubmed: 37138078 ]
- Gr89,696 bound kappa opioid receptor in complex with gustducin (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G alpha gustducin protein (40 kDa, Protein from Homo sapiens)
- Scfv16 protein (26 kDa, Protein from Mus musculus)
- Methyl (3r)-4-[(3,4-dichlorophenyl)acetyl]-3-[(pyrrolidin-1-yl)methyl]piperazine-1-carboxylate (414 Da, Ligand)
- Kappa-type opioid receptor (32 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
GR89,696 bound Kappa Opioid Receptor in complex with Gz
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8dzs
Q-score: 0.531
Han J, Zhang J, Nazarova AL, Bernhard SM, Krumm BE, Zhao L, Lam JH, Rangari VA, Majumdar S, Nichols DE, Katritch V, Yuan P, Fay JF, Che T
Nature (2023) 617 pp. 417-425 [ DOI: doi:10.1038/s41586-023-06030-7 Pubmed: 37138078 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(z) subunit alpha (39 kDa, Protein from Homo sapiens)
- Kappa-type opioid receptor (34 kDa, Protein from Homo sapiens)
- Scfv16 protein (26 kDa, Protein from Mus musculus)
- Methyl (3r)-4-[(3,4-dichlorophenyl)acetyl]-3-[(pyrrolidin-1-yl)methyl]piperazine-1-carboxylate (414 Da, Ligand)
- Gr89,696 bound kappa opioid receptor in complex with gz (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Neurotensin receptor allosterism revealed in complex with a biased allosteric modulator
Resolution: 2.59 Å
EM Method: Single-particle
Fitted PDBs: 8fmz
Q-score: 0.542
Krumm BE, DiBerto JF, Olsen RHJ, Kang HJ, Slocum ST, Zhang S, Strachan RT, Huang XP, Slosky LM, Pinkerton AB, Barak LS, Caron MG, Kenakin T, Fay JF, Roth BL
Biochemistry (2023) 62 pp. 1233-1248 [ DOI: doi:10.1021/acs.biochem.3c00029 Pubmed: 36917754 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- Neurotensin/neuromedin n (819 Da, Protein from Rattus norvegicus)
- Neurotensin receptor type 1 (45 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Minigq (28 kDa, Protein from Escherichia coli)
- Ternary complex of ntsr1 minigq heterotrimer with scfv16 (147 kDa, Complex from Rattus norvegicus)
CryoEM structure of Go-coupled NTSR1 with a biased allosteric modulator
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8fn0
Q-score: 0.478
Krumm BE, DiBerto JF, Olsen RHJ, Kang HJ, Slocum ST, Zhang S, Strachan RT, Huang XP, Slosky LM, Pinkerton AB, Barak LS, Caron MG, Kenakin T, Fay JF, Roth BL
Biochemistry (2023) 62 pp. 1233-1248 [ DOI: doi:10.1021/acs.biochem.3c00029 Pubmed: 36917754 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- Neurotensin/neuromedin n (819 Da, Protein from Rattus norvegicus)
- Ternary complex of ntsr1 minigo heterotrimer with scfv16 (147 kDa, Complex from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Neurotensin receptor type 1 (45 kDa, Protein from Rattus norvegicus)
- Minigo (25 kDa, Protein from Escherichia coli)
CryoEM structure of Go-coupled NTSR1
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 8fn1
Q-score: 0.498
Krumm BE, DiBerto JF, Olsen RHJ, Kang HJ, Slocum ST, Zhang S, Strachan RT, Huang XP, Slosky LM, Pinkerton AB, Barak LS, Caron MG, Kenakin T, Fay JF, Roth BL
Biochemistry (2023) 62 pp. 1233-1248 [ DOI: doi:10.1021/acs.biochem.3c00029 Pubmed: 36917754 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Neurotensin/neuromedin n (819 Da, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(o) subunit alpha (25 kDa, Protein from Homo sapiens)
- Ternary complex of ntsr1 minigo heterotrimer with scfv16 (147 kDa, Complex from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Neurotensin receptor type 1 (45 kDa, Protein from Rattus norvegicus)
- Scfv16 (28 kDa, Protein from Escherichia coli)
CryoEM structure of miniGq-coupled hM3Dq in complex with DCZ
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8e9w
Q-score: 0.543
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 11-(4-methylpiperazin-1-yl)-5h-dibenzo[b,e][1,4]diazepine (292 Da, Ligand)
- Minigq (28 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor 3, minigq protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Gq-coupled hm3r complex (Complex)
- Muscarinic acetylcholine receptor m3 (41 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
CryoEM structure of miniGo-coupled hM4Di in complex with DCZ
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8e9x
Q-score: 0.547
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022) 612 pp. 354-362 [ DOI: doi:10.1038/s41586-022-05489-0 Pubmed: 36450989 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 11-(4-methylpiperazin-1-yl)-5h-dibenzo[b,e][1,4]diazepine (292 Da, Ligand)
- Minigo (25 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Single-chain variable fragment scfv16 (Complex from Mus musculus)
- Gq-coupled hm3r complex (Complex)
- Muscarinic acetylcholine receptor 4, minigo protein, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)