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Structure of an orphan GPCR-G protein signaling complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7wz4
Q-score: 0.523
Chen G, Xu J, Inoue A, Schmidt MF, Bai C, Lu Q, Gmeiner P, Liu Z, Du Y
Nat Commun (2022) 13 pp. 2375-2375 [ DOI: doi:10.1038/s41467-022-30081-5 Pubmed: 35501348 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Probable g-protein coupled receptor 88 (55 kDa, Protein from Homo sapiens)
- Gpr88-gi1 signaling complex (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Gpr88-gi1 (Complex from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7w6p
Q-score: 0.056
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ DOI: doi:10.1126/science.abn7065 Pubmed: 36173843 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Scfv (32 kDa, Protein from Mus musculus)
- N-pyridin-4-ylisoquinolin-4-amine (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)
Cryo-EM structure of the alpha2A adrenergic receptor GoA signaling complex bound to a biased agonist
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7w7e
Q-score: 0.466
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ DOI: doi:10.1126/science.abn7065 Pubmed: 36173843 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Scfv (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 5-(3-bicyclo[4.2.0]octa-1,3,5-trienyl)-1,2,3,6-tetrahydropyridine (185 Da, Ligand)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)
Structure of the alpha2A-adrenergic receptor GoA signaling complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ej0
Q-score: 0.426
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Noradrenaline (170 Da, Ligand)
- Scfv16 (Complex from Mus musculus)
Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to brimonidine
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ej8
Q-score: 0.482
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
- Brimonidine (292 Da, Ligand)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to dexmedetomidine
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7eja
Q-score: 0.378
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- 4-[(1~{s})-1-(2,3-dimethylphenyl)ethyl]-1~{h}-imidazole (200 Da, Ligand)
- Scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to oxymetazoline
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ejk
Q-score: 0.453
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Oxymetazoline (260 Da, Ligand)
- Scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
Structure of the GPR88-Gi1 signaling complex bound to a synthetic ligand
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7ejx
Q-score: 0.606
Chen G, Xu J, Inoue A, Schmidt MF, Bai C, Lu Q, Gmeiner P, Liu Z, Du Y
Nat Commun (2022) 13 pp. 2375-2375 [ DOI: doi:10.1038/s41467-022-30081-5 Pubmed: 35501348 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Gpr88-gi1 signaling complex (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- (1r,2r)-n-[(2s,3s)-2-azanyl-3-methyl-pentyl]-n-[4-(4-propylphenyl)phenyl]-2-pyridin-2-yl-cyclopropane-1-carboxamide (455 Da, Ligand)
- Water (18 Da, Ligand)
- Gpr88-gi1 (Complex from Homo sapiens)
- Probable g-protein coupled receptor 88 (40 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)