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Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 8y6o
Q-score: 0.258
Bai R, Yuan M, Zhang P, Luo T, Shi Y, Wan R
Science (2024) 383 pp. 1245-1252 [ Pubmed: 38484052 DOI: doi:10.1126/science.adn7272 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- U4/u6.u5 tri-snrnp-associated protein 2 (65 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Centrosomal at-ac splicing factor (38 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
- Serine/threonine-protein kinase prp4 homolog (117 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1 (14 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Zinc finger matrin-type protein 5 (20 kDa, Protein from Homo sapiens)
- Pre-mrna (89 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- Human minor pre-b complex (1 MDa, Complex from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- U11 snrna (43 kDa, RNA from Homo sapiens)
- U11/u12 small nuclear ribonucleoprotein 48 kda protein (40 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- U4atac snrna (42 kDa, RNA from Homo sapiens)
- U6atac snrna (40 kDa, RNA from Homo sapiens)
- Thioredoxin-like protein 4b (17 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Programmed cell death protein 7 (54 kDa, Protein from Homo sapiens)
- U11/u12 small nuclear ribonucleoprotein 35 kda protein (29 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- U11/u12 small nuclear ribonucleoprotein 25 kda protein (15 kDa, Protein from Homo sapiens)
Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 7dvq
Q-score: 0.432
Bai R, Wan R, Wang L, Xu K, Zhang Q, Lei J, Shi Y
Science (2021) 371 [ DOI: doi:10.1126/science.abg0879 Pubmed: 33509932 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Armadillo repeat-containing protein 7 (21 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Pre-mrna (44 kDa, RNA from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Sodium channel modifier 1 (25 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Potassium ion (39 Da, Ligand)
- Cysteine-rich pdz-binding protein (11 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Rna-binding protein 48 (41 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- The activated human minor spliceosome (human minor bact complex) (Complex from Homo sapiens)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- 5'-o-[(s)-hydroxy{[(r)-hydroxy{[(s)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine (537 Da, Ligand)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- U6atac snrna (39 kDa, RNA from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- G-patch domain and kow motifs-containing protein (52 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Ring finger protein 113a (38 kDa, Protein from Human)
- U12 snrna (48 kDa, RNA from Homo sapiens)
- Water (18 Da, Ligand)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6j6g
Q-score: 0.317
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ DOI: doi:10.1016/j.cell.2019.02.006 Pubmed: 30879786 ]
- U6 snrna (35 kDa, RNA from Baker's yeast)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Act1 pre-mrna (217 kDa, RNA from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Catalytically activated spliceosome b*-a2 complex (Complex from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6j6h
Q-score: 0.283
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ DOI: doi:10.1016/j.cell.2019.02.006 Pubmed: 30879786 ]
- U6 snrna (35 kDa, RNA from Baker's yeast)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Act1 pre-mrna (217 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- Catalytically activated spliceosome b*-a1 complex (Complex from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 6j6n
Q-score: 0.248
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ DOI: doi:10.1016/j.cell.2019.02.006 Pubmed: 30879786 ]
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Ubc4 pre-mrna (78 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Catalytically activated spliceosome b*-b1 complex (Complex from Saccharomyces cerevisiae S288C)
Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6j6q
Q-score: 0.261
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ DOI: doi:10.1016/j.cell.2019.02.006 Pubmed: 30879786 ]
- Splicing factor yju2 (32 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Catalytically activated spliceosome b*-a2 complex (Complex from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Ubc4 pre-mrna (78 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
cryo-EM structure of a human activated spliceosome (mature Bact) at 5.1 angstrom.
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 5z56
Q-score: 0.131
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Human activated spliceosome (Complex from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Ring finger protein 113a (38 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
Cryo-EM structure of the human activated spliceosome (late Bact) at 6.5 angstrom
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 5z57
Q-score: 0.097
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Alanine (89 Da, Ligand)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Late bact splieosome (Complex from Homo sapiens)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 5z58
Q-score: 0.131
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Early bact spliceosome (Complex from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Ring finger protein 113a (38 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5zwm
Q-score: 0.162
Bai R, Wan R, Yan C, Lei J, Shi Y
Science (2018) 360 pp. 1423-1429 [ DOI: doi:10.1126/science.aau0325 Pubmed: 29794219 ]
- U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Yeast fully assembled spliceosomal pre-b complex before activation (Cell from Saccharomyces cerevisiae)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor rds3 (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Baker's yeast)
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Baker's yeast)
- U2 snrnp component ist3 (17 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Baker's yeast)
- U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Baker's yeast)
- 66 kda u4/u6.u5 small nuclear ribonucleoprotein component (66 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Baker's yeast)
- U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor prp21 (33 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 6 (104 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor rse1 (153 kDa, Protein from Baker's yeast)
- U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Baker's yeast)
- Spliceosomal protein dib1 (16 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp9 (63 kDa, Protein from Baker's yeast)
- U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Protein hsh49 (24 kDa, Protein from Baker's yeast)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp11 (29 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Pre-mrna-bps (13 kDa, RNA from Saccharomyces cerevisiae S288c)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- U2 snrnp component hsh155 (110 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Rds3 complex subunit 10 (10 kDa, Protein from Baker's yeast)
- Pre-mrna leakage protein 1 (23 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 31 (56 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc26 (30 kDa, Protein from Baker's yeast)