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Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound, composite map
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7b9v
Q-score: 0.378
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439- [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
- Branched intron and 3' exon of ubc4 pre-mrna (30 kDa, RNA from Saccharomyces cerevisiae)
- Bj4_g0054360.mrna.1.cds.1 (50 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Snt309 isoform 1 (20 kDa, Protein from Baker's yeast)
- D-chiro inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-splicing factor cwc25 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (121 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Y55_g0042700.mrna.1.cds.1 (67 kDa, Protein from Baker's yeast)
- Unassigned structure (20 kDa, Protein from Saccharomyces cerevisiae)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Potassium ion (39 Da, Ligand)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Hlj1_g0053790.mrna.1.cds.1 (27 kDa, Protein from Baker's yeast)
- Cdc40 isoform 1 (52 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
- Cwc22 isoform 1 (67 kDa, Protein from Baker's yeast)
- Syf1 isoform 1 (100 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 18 (28 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Bj4_g0027490.mrna.1.cds.1 (12 kDa, Protein from Baker's yeast)
- 5' exon of ubc4 mrna (15 kDa, RNA from Saccharomyces cerevisiae)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor isy1 (27 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Clf1 isoform 1 (82 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Splicing factor yju2 (33 kDa, Protein from Baker's yeast)
- Bud31 isoform 1 (18 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Baker's yeast)
- Ntc20 isoform 1 (15 kDa, Protein from Baker's yeast)
- Yeast c complex spliceosome with prp18/slu7 bound (2 MDa, Complex from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
Human C Complex Spliceosome - High-resolution CORE
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6zym
Q-score: 0.498
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ DOI: doi:10.1016/j.molcel.2020.09.012 Pubmed: 33007253 ]
- Pre-mrna-processing-splicing factor 8 (207 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (25 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U5 snrna (36 kDa, RNA from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (107 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Wd repeat-containing protein 70 (73 kDa, Protein from Human)
- U6 snrna (25 kDa, RNA from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Splicing factor yju2 (21 kDa, Protein from Human)
- Corepressor interacting with rbpj 1 (52 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor cwc25 homolog (49 kDa, Protein from Human)
- Protein frg1 (29 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Human c complex spliceosome - high resolution core (Complex from Homo sapiens)
- Pre-mrna (103 kDa, RNA from Human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (140 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6j6q
Q-score: 0.261
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ DOI: doi:10.1016/j.cell.2019.02.006 Pubmed: 30879786 ]
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- Catalytically activated spliceosome b*-a2 complex (Complex from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Splicing factor yju2 (32 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ubc4 pre-mrna (78 kDa, RNA from Saccharomyces cerevisiae S288c)
- Zinc ion (65 Da, Ligand)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)