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Cryo-EM structure of Xenopus Patched-1 in complex with GAS1 and Sonic Hedgehog
Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 7rhq
Q-score: 0.471
Huang P, Wierbowski BM, Lian T, Chan C, Garcia-Linares S, Jiang J, Salic A
Dev Cell (2022) 57 pp. 670-685.e8 [ Pubmed: 35231446 DOI: doi:10.1016/j.devcel.2022.02.008 ]
- Growth arrest-specific protein 1 (29 kDa, Protein from Homo sapiens)
- Sonic hedgehog protein n-product (21 kDa, Protein from Homo sapiens)
- Patched hedgehog gas1 complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Patched-1 (134 kDa, Protein from Xenopus laevis)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of hDisp1NNN-ShhN
Resolution: 4.07 Å
EM Method: Single-particle
Fitted PDBs: 7e2i
Q-score: 0.375
Li W, Wang L, Wierbowski BM, Lu M, Dong F, Liu W, Li S, Wang P, Salic A, Gong X
Nat Commun (2021) 12 pp. 6966-6966 [ Pubmed: 34845226 DOI: doi:10.1038/s41467-021-27257-w ]
- Protein dispatched homolog 1 (171 kDa, Protein from Homo sapiens)
- Hdisp1nnn (Complex from Homo sapiens)
- Shhn (Complex from Homo sapiens)
- Hdisp1nnn-shhn (150 kDa, Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Sonic hedgehog protein (49 kDa, Protein from Homo sapiens)
Cryo-EM structure of murine Dispatched in complex with Sonic hedgehog
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7rpk
Q-score: 0.547
Wang Q, Asarnow DE, Ding K, Mann RK, Hatakeyama J, Zhang Y, Ma Y, Cheng Y, Beachy PA
Nature (2021) 599 pp. 320-324 [ DOI: doi:10.1038/s41586-021-03996-0 Pubmed: 34707294 ]
- (2s)-3-{[(s)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(hexanoyloxy)propyl hexanoate (411 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sonic hedgehog protein (18 kDa, Protein from Mus musculus)
- Dispatched protein complexed with sonic hedgehog 26-189 (151 kDa, Complex from Mus musculus)
- Protein dispatched homolog 1 (152 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Sulfate ion (96 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7mhz
Q-score: 0.509
Science (2021) 372 pp. 1215-1219 [ DOI: doi:10.1126/science.abg4998 Pubmed: 34112694 ]
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Hhat in complex with a palmitoylated hedgehog peptide product and a fab antibody fragment (Complex from Homo sapiens)
- Sonic hedgehog protein n-product peptide (750 Da, Protein from Homo sapiens)
- Protein-cysteine n-palmitoyltransferase hhat (57 kDa, Protein from Homo sapiens)
- 3h02 fab heavy chain (51 kDa, Protein from Mus musculus)
- Palmitoyl-coa (1 kDa, Ligand)
- Digitonin (1 kDa, Ligand)
- Palmitic acid (256 Da, Ligand)
- 3h02 fab light chain (22 kDa, Protein from Mus musculus)
Structure of PTCH1 bound to a modified Hedgehog ligand ShhN-C24II
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6rmg
Q-score: 0.474
Qi C, Di Minin G, Vercellino I, Wutz A, Korkhov VM
Sci Adv (2019) 5 pp. eaaw6490-eaaw6490 [ Pubmed: 31555730 DOI: doi:10.1126/sciadv.aaw6490 ]
- Sonic hedgehog protein (32 kDa, Protein from Homo sapiens)
- Sonic hedgehog protein (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Protein patched homolog 1,gfp-like fluorescent chromoprotein fp506, related (163 kDa, Protein from Eimeria acervulina)
- Protein patched homolog 1 + gfp (Complex from Eimeria acervulina)
- Complex of ptch1 with a modified hedgehog ligand shhn-c24ii (Complex)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Qi C, Di Minin G, Vercellino I, Wutz A, Korkhov VM
Sci Adv (2019) 5 pp. eaaw6490-eaaw6490 [ Pubmed: 31555730 DOI: doi:10.1126/sciadv.aaw6490 ]
Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form I)
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 6n7g
Q-score: 0.132
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun (2019) 10 pp. 2320-2320 [ DOI: doi:10.1038/s41467-019-10234-9 Pubmed: 31127104 ]
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
- Patch1 (Cellular component from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of the 2:1 hPtch1-Shhp complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6n7h
Q-score: 0.408
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun (2019) 10 pp. 2320-2320 [ DOI: doi:10.1038/s41467-019-10234-9 Pubmed: 31127104 ]
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
- Patch1 (Cellular component from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form II)
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 6n7k
Q-score: 0.132
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun (2019) 10 pp. 2320-2320 [ DOI: doi:10.1038/s41467-019-10234-9 Pubmed: 31127104 ]
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
- Patch1 (Cellular component from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of 2:1 human Ptch1-Shh-N complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6e1h
Q-score: 0.394
Qi X, Schmiege P, Coutavas E, Li X
Science (2018) 362 [ Pubmed: 30139912 DOI: doi:10.1126/science.aas8843 ]
- Protein patched homolog 1 (160 kDa, Protein from Homo sapiens)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
- Ptc (265 kDa, Complex from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)