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Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8r6u
Q-score: 0.565
Williams HM, Thorkelsson SR, Vogel D, Busch C, Milewski M, Cusack S, Grunewald K, Quemin ERJ, Rosenthal M
Nucleic Acids Res (2024) [ DOI: doi:10.1093/nar/gkae330 Pubmed: 38709882 ]
- Rna-directed rna polymerase l (235 kDa, Protein from SFTS virus AH12)
- Sftsv l protein in complex with 5' and 3' rna, as well as rna acting as a primer. (240 kDa, Complex from SFTS virus AH12)
- Rna primer (6 kDa, RNA from SFTS virus AH12)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*cp*up*gp*gp*gp*cp*gp*gp*up*cp*up*up*up*gp*up*gp*u)-3') (8 kDa, RNA from SFTS virus AH12)
- Rna primer tail-end (5 kDa, RNA from SFTS virus AH12)
Cryo-EM structure of the Mismatch Locking Complex (III) of Human Mitochondrial DNA Polymerase Gamma
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 8g5m
Q-score: 0.434
Buchel G, Nayak AR, Herbine K, Sarfallah A, Sokolova VO, Zamudio-Ochoa A, Temiakov D
Nat Commun (2023) 14 pp. 8501-8501 [ Pubmed: 38151585 DOI: doi:10.1038/s41467-023-44198-8 ]
- Cryo-em structure of the mismatch locking complex (iii) of human mitochondrial dna polymerase gamma (362 kDa, Complex from Homo sapiens)
- Template dna (9 kDa, DNA from synthetic construct)
- Mismatched rna primer (7 kDa, RNA from synthetic RNA)
- Dna polymerase subunit gamma-2, mitochondrial (54 kDa, Protein from Homo sapiens)
- Dna polymerase subunit gamma-1 (139 kDa, Protein from Homo sapiens)
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 8g5n
Q-score: 0.384
Buchel G, Nayak AR, Herbine K, Sarfallah A, Sokolova VO, Zamudio-Ochoa A, Temiakov D
Nat Commun (2023) 14 pp. 8501-8501 [ Pubmed: 38151585 DOI: doi:10.1038/s41467-023-44198-8 ]
- Template dna (9 kDa, DNA from synthetic construct)
- Cryo-em structure of the guide loop engagement complex (vi) of human mitochondrial dna polymerase gamma (362 kDa, Complex from Homo sapiens)
- Dna polymerase subunit gamma-2, mitochondrial (54 kDa, Protein from Homo sapiens)
- Mismatched rna primer (7 kDa, RNA from synthetic RNA)
- Dna polymerase subunit gamma-1 (139 kDa, Protein from Homo sapiens)
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 8g5o
Q-score: 0.36
Buchel G, Nayak AR, Herbine K, Sarfallah A, Sokolova VO, Zamudio-Ochoa A, Temiakov D
Nat Commun (2023) 14 pp. 8501-8501 [ Pubmed: 38151585 DOI: doi:10.1038/s41467-023-44198-8 ]
- Dna polymerase subunit gamma-1 (139 kDa, Protein from Homo sapiens)
- Template dna (9 kDa, DNA from synthetic construct)
- Cryo-em structure of the guide loop engagement complex (iv) of human mitochondrial dna polymerase gamma (362 kDa, Complex from Homo sapiens)
- Mismatched rna primer (7 kDa, RNA from synthetic RNA)
- Dna polymerase subunit gamma-2, mitochondrial (54 kDa, Protein from Homo sapiens)
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 8g5p
Q-score: 0.411
Buchel G, Nayak AR, Herbine K, Sarfallah A, Sokolova VO, Zamudio-Ochoa A, Temiakov D
Nat Commun (2023) 14 pp. 8501-8501 [ Pubmed: 38151585 DOI: doi:10.1038/s41467-023-44198-8 ]
- Template dna (9 kDa, DNA from synthetic construct)
- Cryo-em structure of the guide loop engagement complex (v) of human mitochondrial dna polymerase gamma (362 kDa, Complex from Homo sapiens)
- Dna polymerase subunit gamma-2, mitochondrial (54 kDa, Protein from Homo sapiens)
- Mismatched rna primer (7 kDa, RNA from synthetic RNA)
- Dna polymerase subunit gamma-1 (139 kDa, Protein from Homo sapiens)
DNA initiation complex (configuration 1) of Xenopus laevis DNA polymerase alpha-primase
Resolution: 11.16 Å
EM Method: Single-particle
Fitted PDBs: 8v6g
Q-score: 0.051
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) [ Pubmed: 38491139 DOI: doi:10.1038/s41594-024-01227-4 ]
- Dna primase (49 kDa, Protein from Xenopus laevis)
- Dna template (15 kDa, DNA from synthetic construct)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
- Primase (Complex from Xenopus laevis)
- Rna primer (2 kDa, RNA from synthetic construct)
- Polymerase alpha-primase with a dna initiation substrate (110 kDa, Complex)
- Polymerase alpha (Complex from Xenopus laevis)
- Magnesium ion (24 Da, Ligand)
- Dna initiation substrate (20 kDa, Complex from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
DNA initiation complex (configuration 2) of Xenopus laevis DNA polymerase alpha-primase
Resolution: 11.11 Å
EM Method: Single-particle
Fitted PDBs: 8v6h
Q-score: 0.02
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) [ Pubmed: 38491139 DOI: doi:10.1038/s41594-024-01227-4 ]
- Dna primase (49 kDa, Protein from Xenopus laevis)
- Dna template (15 kDa, DNA from synthetic construct)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
- Primase (Complex from Xenopus laevis)
- Rna primer (2 kDa, RNA from synthetic construct)
- Polymerase alpha-primase with a dna initiation substrate (110 kDa, Complex)
- Polymerase alpha (Complex from Xenopus laevis)
- Magnesium ion (24 Da, Ligand)
- Dna initiation substrate (20 kDa, Complex from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
DNA initiation subcomplex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8g9l
Q-score: 0.453
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) [ Pubmed: 38491139 DOI: doi:10.1038/s41594-024-01227-4 ]
- Dna template (15 kDa, DNA from synthetic construct)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Primase (Complex from Xenopus laevis)
- Rna primer (2 kDa, RNA from synthetic construct)
- Polymerase alpha-primase with a dna initiation substrate (110 kDa, Complex)
- Polymerase alpha (Complex from Xenopus laevis)
- Magnesium ion (24 Da, Ligand)
- Dna initiation substrate (20 kDa, Complex from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Gene product 4 helicase-primase (Protein from Enterobacteria phage T7)
- Rna primer (RNA from Enterobacteria phage T7)
- Gene product 5 dna polymerase (Protein from Enterobacteria phage T7)
- Two t7 dna polymerase/trx complexes interacting with primase domains of t7 helicase (gp4), the first with primase a and b in complex with rna/dna hybrid and dttp, and the second one with only primase c and d (from gp4-gp5 lagging-strand complex lags4) (Complex from Enterobacteria phage T7)
- Dna (DNA from Enterobacteria phage T7)