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cryo-EM structure of the human spliceosomal B complex protomer (tri-snRNP core region)
Zhang Z, Kumar V, Dybkov O, Will CL, Urlaub H, Stark H, Luehrmann R
To Be Published
- Transcription elongation regulator 1 (124 kDa, Protein from Homo sapiens)
- Ww domain-binding protein 4 (42 kDa, Protein from Homo sapiens)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Minx pre-mrna (111 kDa, RNA from Homo sapiens)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Human pre-catalytic spliceosome (Complex from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Homo sapiens)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Nhp2-like protein 1, n-terminally processed (14 kDa, Protein from Homo sapiens)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- Ubiquitin-like protein 5 (8 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
Cryo-EM structure of a human spliceosomal B complex protomer
Resolution: 5.29 Å
EM Method: Single-particle
Fitted PDBs: 8qo9
Q-score: 0.158
Zhang Z, Kumar V, Dybkov O, Will CL, Urlaub H, Stark H, Luehrmann R
To Be Published
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- Human spliceosomal b complex (Complex from Homo sapiens)
- Transcription elongation regulator 1 (124 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
- Ww domain-binding protein 4 (42 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Minx pre-mrna (111 kDa, RNA from Homo sapiens)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase h (19 kDa, Protein from Homo sapiens)
- Serine/arginine-rich splicing factor 1 (27 kDa, Protein from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
- Wd40 repeat-containing protein smu1 (57 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Homo sapiens)
- Protein red (65 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1, n-terminally processed (14 kDa, Protein from Homo sapiens)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
- Ubiquitin-like protein 5 (8 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
Human spliceosomal PM5 C* complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8c6j
Q-score: 0.353
Dybkov O, Preussner M, El Ayoubi L, Feng VY, Harnisch C, Merz K, Leupold P, Yudichev P, Agafonov DE, Will CL, Girard C, Dienemann C, Urlaub H, Kastner B, Heyd F, Luehrmann R
Sci Adv (2023) 9 pp. eadf1785- [ DOI: doi:10.1126/sciadv.adf1785 Pubmed: 36867703 ]
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (57 kDa, Protein from human)
- U5 snrna (36 kDa, RNA from human)
- Crooked neck-like protein 1 (83 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Intron-binding protein aquarius (171 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Pm5 5' exon (78 kDa, RNA from Homo sapiens)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Human spliceosomal c* complex assembled on pm5 pre-mrna (Complex from Homo sapiens)
- Nitric oxide synthase-interacting protein (33 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Potassium ion (39 Da, Ligand)
- Splicing factor ess-2 homolog (52 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 3 (18 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pm5 intron (74 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Sting er exit protein (25 kDa, Protein from human)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Splicing regulator sde2 (49 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Probable atp-dependent rna helicase ddx41 (69 kDa, Protein from human)
- Protein fam50a (40 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Splicing factor c9orf78 (33 kDa, Protein from human)
- Splicing factor cactin (88 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Nf-kappa-b-activating protein (47 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
Structural organization of a late activated human spliceosome (Baqr, core region)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qtt
Q-score: 0.384
Schmitzova J, Cretu C, Dienemann C, Urlaub H, Pena V
Nature (2023) 617 pp. 842-850 [ Pubmed: 37165190 DOI: doi:10.1038/s41586-023-06049-w ]
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Bud13 homolog (70 kDa, Protein from human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Minx (102 kDa, RNA from unidentified adenovirus)
- Ring finger protein 113a (38 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- U2snrna (60 kDa, RNA from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- G-patch domain and kow motifs-containing protein (52 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 4 (57 kDa, Protein from human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Late human activated spliceosome arrested with a dominant-negative mutant of the splicing helicase aquarius (aquarius k829a) (Complex from Homo sapiens)
- U6snrna (34 kDa, RNA from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- U5 snrna (37 kDa, RNA from human)
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 8ch6
Q-score: 0.154
Schmitzova J, Cretu C, Dienemann C, Urlaub H, Pena V
Nature (2023) 617 pp. 842-850 [ Pubmed: 37165190 DOI: doi:10.1038/s41586-023-06049-w ]
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from human)
- Intron-binding protein aquarius (171 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Bud13 homolog (70 kDa, Protein from human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Rnu5a-1 (37 kDa, RNA from human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Ring finger protein 113a (38 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Minx-m3 (102 kDa, RNA from unidentified adenovirus)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- G-patch domain and kow motifs-containing protein (52 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 4 (57 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from human)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Rnu2-1 (60 kDa, RNA from human)
- Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Late human activated spliceosome arrested with a dominant-negative mutant of the splicing helicase aquarius (aquarius k829a) (Complex from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Rnu6-1 (34 kDa, RNA from Homo sapiens)
The cryo-EM structure of human pre-C*-I complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7w59
Q-score: 0.268
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ DOI: doi:10.1016/j.molcel.2022.05.021 Pubmed: 35705093 ]
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- Protein bud31 homolog (17 kDa, Protein from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- Human pre-c*-i complex (Complex from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Rna helicase aquarius (171 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Psme3-interacting protein (28 kDa, Protein from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- U5 snrna (37 kDa, RNA from human)
The cryo-EM structure of human pre-C*-II complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7w5a
Q-score: 0.269
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ DOI: doi:10.1016/j.molcel.2022.05.021 Pubmed: 35705093 ]
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Cactin (88 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Human pre-c*-ii complex (Complex from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Psme3-interacting protein (28 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- U5 snrna (37 kDa, RNA from human)
- U2 snrna (60 kDa, RNA from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- Rna helicase aquarius (171 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
The cryo-EM structure of human C* complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7w5b
Q-score: 0.192
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ DOI: doi:10.1016/j.molcel.2022.05.021 Pubmed: 35705093 ]
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Cactin (88 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Human pre-c*-i complex (Complex from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- Rna helicase aquarius (171 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Nf-kappa-b-activating protein (47 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- U5 snrna (37 kDa, RNA from human)
- Protein fam32a (13 kDa, Protein from human)
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7abi
Q-score: 0.152
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- U5 snrna (36 kDa, RNA from Human)
- Human pre-bact-2 spliceosome (Complex)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Ubiquitin-like protein 5 (8 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Dna/rna-binding protein kin17 (45 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Minx m3 pre-mrna (103 kDa, RNA from synthetic construct)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Beta-catenin-like protein 1 (65 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Minx m3 pre-mrna (Complex from synthetic construct)
- Human pre-bact-2 spliceosome (Complex from Homo sapiens)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
Resolution: 7.8 Å
EM Method: Single-particle
Fitted PDBs: 7abg
Q-score: 0.147
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Transcription elongation regulator 1 (124 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
- U5 snrna (36 kDa, RNA from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- Ww domain-binding protein 11 (70 kDa, Protein from Homo sapiens)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Homo sapiens)
- 7-methyl-guanosine-5'-triphosphate-5'-guanosine (803 Da, Ligand)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Minx m3 pre-mrna (73 kDa, RNA from synthetic construct)
- Pre-bact-1 spliceosomal complex (Complex)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
- Serine/arginine-rich splicing factor 1 (27 kDa, Protein from Homo sapiens)
- Pre-bact-1 spliceosomal complex (Complex from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 2 (18 kDa, Protein from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from Homo sapiens)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 1 (91 kDa, Protein from Homo sapiens)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
- Ubiquitin-like protein 5 (8 kDa, Protein from Homo sapiens)
- Snw domain-containing protein 1 (61 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)