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sample_name:"Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22"

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EMD-6839

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Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom

Release date: July 18, 2018
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 5ylz
Q-score: 0.251

Bai R, Yan C, Wan R, Lei J, Shi Y

Cell (2017) 171 pp. 1589-1598.e8 [ DOI: doi:10.1016/j.cell.2017.10.038 Pubmed: 29153833 ]

Sample:

Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-processing factor 17 (52 kDa, Protein from Saccharomyces cerevisiae S288c)
Mrna/intron lariat (113 kDa, RNA from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
Zinc ion (65 Da, Ligand)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae S288c)
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor cef1 (67 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 18 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Saccharomyces cerevisiae S288c)
U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor snt309 (20 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288c)
Inositol hexakisphosphate (660 Da, Ligand)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288c)
Magnesium ion (24 Da, Ligand)
Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae S288c)
The post-catalytic spliceosome (p complex) (Complex from Saccharomyces cerevisiae S288c)

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EMD-7109

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S. cerevisiae spliceosomal post-catalytic P complex

Release date: Feb. 21, 2018
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6bk8
Q-score: 0.38

Liu S, Li X, Zhang L, Jiang J, Hill RC, Cui Y, Hansen KC, Zhou ZH, Zhao R

Science (2017) 358 pp. 1278-1283 [ DOI: doi:10.1126/science.aar3462 Pubmed: 29146870 ]

Sample:

Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
Inositol hexakisphosphate (660 Da, Ligand)
Rna (59-mer) (18 kDa, RNA from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
Unknown protein fragment (1 kDa, Protein from Saccharomyces cerevisiae)
Zinc ion (65 Da, Ligand)
Lea1 (12 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor prp17 (52 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor prp19 (56 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor prp18 (28 kDa, Protein from Baker's yeast)
Spliceosomal p complex (Complex from Saccharomyces cerevisiae)
U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
Unknown protein fragment (18 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
Magnesium ion (24 Da, Ligand)
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp8 (279 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Msl1 (27 kDa, Protein from Baker's yeast)
Rna (34-mer) (10 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)

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EMD-3979

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Post-catalytic P complex spliceosome with 3' splice site docked

Release date: Jan. 17, 2018
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6exn
Q-score: 0.296

Wilkinson ME, Fica SM, Galej WP, Norman CM, Newman AJ, Nagai K

Science (2017) 358 pp. 1283-1288 [ DOI: doi:10.1126/science.aar3729 Pubmed: 29146871 ]

Sample:

Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
Inositol hexakisphosphate (660 Da, Ligand)
Pre-mrna-splicing factor cwc16 (32 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
U5 snrna (57 kDa, RNA from Baker's yeast)
Zinc ion (65 Da, Ligand)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
U6 snrna (35 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
Unassigned structure (8 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor prp17 (52 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor prp19 (56 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
Intron lariat: ubc4 rna (30 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp18 (28 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
P complex spliceosome with 3' exon docked (2 MDa, Complex)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
Magnesium ion (24 Da, Ligand)
Ligated exons: ubc4 mrna (12 kDa, RNA from Saccharomyces cerevisiae S288c)
P complex spliceosome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor prp8 (279 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
3' exon (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

[Show more]

EMD-3541

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Structure of a spliceosome remodeled for exon ligation

Release date: Jan. 18, 2017
Resolution: 4.17 Å
EM Method: Single-particle
Fitted PDBs: 5mq0
Q-score: 0.223

Fica SM, Oubridge C, Galej WP, Wilkinson ME, Bai XC, Newman AJ, Nagai K

Nature (2017) 542 pp. 377-380 [ DOI: doi:10.1038/nature21078 Pubmed: 28076345 ]

Sample:

Guanosine-5'-triphosphate (523 Da, Ligand)
Yeast ubc4 gene for ubiquitin-conjugating enzyme (30 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae strain wi_c_mbsp_4 chromosome vii sequence (57 kDa, RNA from Baker's yeast)
Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
Inositol hexakisphosphate (660 Da, Ligand)
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
Unknown protein (5 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
Pre-mrna-splicing factor syf1,pre-mrna-splicing factor syf1 (101 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor 18 (28 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae strain t.52_2h chromosome xii sequence (35 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
S.cerevisiae chromosome ii reading frame orf ybr230c (376 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Baker's yeast)
Potassium ion (39 Da, Ligand)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
5'-exon of ubc4 pre-mrna (6 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae spliceosome. complex c just after prp16-mediated remodeling (Complex)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
3'-exon of ubc4 pre-mrna, bound by prp22 helicase (873 Da, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
Magnesium ion (24 Da, Ligand)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)

[Show more]

EMD-3542

Download Map 3D View Raw data

Structure of a spliceosome remodeled for exon ligation

Release date: Jan. 18, 2017
Resolution: 4.6 Å
EM Method: Single-particle

Fica SM, Galej WP, Wilkinson ME, Oubridge C, Bai X-C, Newman AJ, Nagai K

Nature (2017) 542 pp. 377-380 [ DOI: doi:10.1038/nature21078 Pubmed: 28076345 ]

Sample:

Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
U6 snrna (35 kDa, RNA from Baker's yeast)
Inositol hexakisphosphate (660 Da, Ligand)
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
Intron of ubc4 pre-mrna (30 kDa, RNA from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
U2 snrna (376 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor 18 (28 kDa, Protein from Baker's yeast)
Unknown protein (5 kDa, Protein from Baker's yeast)
5'exon of ubc4 pre-mrna (6 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
U5 snrna (57 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Baker's yeast)
Potassium ion (39 Da, Ligand)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
3'exon of ubc4 pre-mrna bound to prp22 helicase (873 Da, RNA from Saccharomyces cerevisiae)
Saccharomyces cerevisiae spliceosome. complex c just after prp16-mediated remodeling (Complex)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
Magnesium ion (24 Da, Ligand)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)

[Show more]

Sort by: Download

Showing 1 - 5 of 5. Page 1 of 1

Display: Statistics

Download

Select database:

EMDB [5]

Format:

Select the script type:

Shell (Unix-based) Batch (Windows-based)

Preview first 10

Field: