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sample_name:"Mediator of RNA polymerase II transcription subunit 31" AND xref_links:"uniprot"

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EMD-16610

Download Map 3D View

Yeast RNA polymerase II transcription pre-initiation complex with core Mediator

Release date: March 22, 2023
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8cen
Q-score: 0.269

Schilbach S, Wang H, Dienemann C, Cramer P

PNAS (2023) 120 pp. e2220542120-e2220542120 [ Pubmed: 37014863 DOI: doi:10.1073/pnas.2220542120 ]

Sample:

Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from baker's yeast)
Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from baker's yeast)
Dna-directed rna polymerase ii subunit rpb2 (138 kDa, Protein from baker's yeast)
General transcription and dna repair factor iih helicase subunit xpd (89 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from baker's yeast)
Dna-directed rna polymerase ii subunit rpb3 (38 kDa, Protein from baker's yeast)
Transcription initiation factor iif subunit beta (46 kDa, Protein from Saccharomyces cerevisiae)
General transcription and dna repair factor iih subunit tfb4 (37 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from baker's yeast)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from baker's yeast)
Dna-directed rna polymerase ii subunit rpb1 (191 kDa, Protein from baker's yeast)
Transcription initiation factor iie subunit beta (37 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 8 (25 kDa, Protein from Saccharomyces cerevisiae)
General transcription and dna repair factor iih subunit tfb1 (72 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iib (39 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 6 (32 kDa, Protein from Saccharomyces cerevisiae)
Template dna (209-mer) (64 kDa, DNA from synthetic construct)
Dna (Complex from Synthetic construct)
Mediator of rna polymerase ii transcription subunit 1 (64 kDa, Protein from Saccharomyces cerevisiae)
Rna polymerase ii transcription factor b subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
Yeast rna polymerase ii transcription pre-initiation complex with core mediator (2 MDa, Complex)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Saccharomyces cerevisiae)
General transcription and dna repair factors, and rna polymerase ii transcription factor b subunit 3 (Complex from Saccharomyces cerevisiae)
Transcription initiation factors and mediator of rna polymerase ii transcription subunits (Complex from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 7 (25 kDa, Protein from Saccharomyces cerevisiae)
Iron/sulfur cluster (351 Da, Ligand)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from baker's yeast)
General transcription and dna repair factor iih helicase subunit xpb (95 kDa, Protein from Saccharomyces cerevisiae)
General transcription and dna repair factor iih subunit tfb2 (58 kDa, Protein from Saccharomyces cerevisiae)
Nontemplate dna (209-mer) (64 kDa, DNA from synthetic construct)
Mediator of rna polymerase ii transcription subunit 20 (22 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 31 (14 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 9 (17 kDa, Protein from Saccharomyces cerevisiae)
Zinc ion (65 Da, Ligand)
Tata-binding protein (27 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iif subunit alpha (82 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iia subunit 2 (13 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iie subunit alpha (55 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 17 (78 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerase subunits (Complex from Saccharomyces cerevisiae)
Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from baker's yeast)
General transcription and dna repair factor iih subunit ssl1 (52 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iia large subunit (32 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 14 (84 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 19 (24 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 21 (16 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 10 (17 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 18 (34 kDa, Protein from Saccharomyces cerevisiae)
General transcription and dna repair factor iih subunit tfb5 (8 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from baker's yeast)
Magnesium ion (24 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 4 (32 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 22 (13 kDa, Protein from Saccharomyces cerevisiae)

[Show more]

EMD-16611

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Yeast RNA polymerase II transcription pre-initiation complex with core Mediator and the +1 nucleosome

Release date: March 8, 2023
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8ceo
Q-score: 0.222

Schilbach S, Wang H, Dienemann C, Cramer P

PNAS (2023) 120 pp. e2220542120-e2220542120 [ Pubmed: 37014863 DOI: doi:10.1073/pnas.2220542120 ]

Sample:

Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from baker's yeast)
Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from baker's yeast)
Dna-directed rna polymerase ii subunit rpb2 (138 kDa, Protein from baker's yeast)
Tfb1 isoform 1 (72 kDa, Protein from Saccharomyces cerevisiae)
General transcription and dna repair factor iih helicase subunit xpd (89 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from baker's yeast)
General transcription and dna repair factor iih (52 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iif subunit beta (46 kDa, Protein from Saccharomyces cerevisiae)
General transcription and dna repair factor iih subunit tfb4 (37 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from baker's yeast)
Histone h2a (13 kDa, Protein from Xenopus laevis)
Histone h4 (11 kDa, Protein from Xenopus laevis)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from baker's yeast)
Dna-directed rna polymerase ii subunit rpb1 (191 kDa, Protein from baker's yeast)
Transcription initiation factor iie subunit beta (37 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 14 (123 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 8 (25 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerase ii subunit rpb3 (38 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iib (39 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 6 (32 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 1 (64 kDa, Protein from Saccharomyces cerevisiae)
Rna polymerase ii transcription factor b subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
Histone h3.2 (15 kDa, Protein from Xenopus laevis)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Saccharomyces cerevisiae)
Histone h2b (13 kDa, Protein from Saccharomyces cerevisiae)
Toa1 isoform 1 (32 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 7 (25 kDa, Protein from Saccharomyces cerevisiae)
Iron/sulfur cluster (351 Da, Ligand)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from baker's yeast)
General transcription and dna repair factor iih helicase subunit xpb (95 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 20 (22 kDa, Protein from Saccharomyces cerevisiae)
Rna polymerase ii transcription factor b subunit 2 (58 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 31 (14 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 9 (17 kDa, Protein from Saccharomyces cerevisiae)
Zinc ion (65 Da, Ligand)
Tata-binding protein (27 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iif subunit alpha (82 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iia subunit 2 (13 kDa, Protein from Saccharomyces cerevisiae)
Transcription initiation factor iie subunit alpha (55 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 17 (78 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 19 (24 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 21 (16 kDa, Protein from Saccharomyces cerevisiae)
Yeast rna polymerase ii transcription pre-initiation complex with core mediator and the +1 nucleosome (2 MDa, Complex from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 10 (17 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 18 (34 kDa, Protein from Saccharomyces cerevisiae)
General transcription and dna repair factor iih subunit tfb5 (8 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae)
Nontemplate dna (209-mer) (64 kDa, DNA from synthetic construct)
Template dna (209-mer) (64 kDa, DNA from synthetic construct)
Magnesium ion (24 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 4 (32 kDa, Protein from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 22 (13 kDa, Protein from Saccharomyces cerevisiae)

[Show more]

EMD-26551

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Mediator-PIC Early (Composite Model)

Release date: March 1, 2023
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7uio
Q-score: 0.239

Gorbea Colon JJ, Palao 3rd L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K

Mol Cell (2023) 83 pp. 574- [ DOI: doi:10.1016/j.molcel.2023.01.011 Pubmed: 36731470 ]

Sample:

Mediator of rna polymerase ii transcription subunit 2 (47 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 14 (123 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb3 (35 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription initiation factor iif subunit alpha (82 kDa, Protein from Saccharomyces cerevisiae S288C)
Aspartic acid (133 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 20 (22 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 7 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Regulatory protein gal4 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Alanine (89 Da, Ligand)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna (37-mer) (11 kDa, DNA from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
Threonine (119 Da, Ligand)
Transcription elongation factor s-ii (34 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 4 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 19 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 18 (34 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 17 (78 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 16 (111 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb1 (162 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription initiation factor iib (38 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription initiation factor iif subunit beta (46 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 21 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb1 (2 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 6 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 3 (43 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 15 (120 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 8 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Zinc ion (65 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 10 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 5 (128 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb2 (138 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna (38-mer) (11 kDa, DNA from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 22 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 9 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 31 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 1 (64 kDa, Protein from Saccharomyces cerevisiae S288C)
Med-picearly (Complex from Saccharomyces cerevisiae S288C)

[Show more]

EMD-26542

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Core Mediator-PICearly (Copy A)

Release date: Feb. 15, 2023
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ui9
Q-score: 0.294

Gorbea Colon JJ, Palao 3rd L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K

Mol Cell (2023) 83 pp. 574- [ DOI: doi:10.1016/j.molcel.2023.01.011 Pubmed: 36731470 ]

Sample:

Mediator of rna polymerase ii transcription subunit 14 (123 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb3 (35 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription initiation factor iif subunit alpha (82 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 20 (22 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 7 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
Core mediator-picearly (copy a) (Complex from Saccharomyces cerevisiae S288C)
Transcription elongation factor s-ii (34 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 4 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 19 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 18 (34 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 17 (78 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription initiation factor iif subunit beta (46 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription initiation factor iib (38 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb1 (162 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 21 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb1 (2 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 6 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 8 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 10 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb2 (138 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 22 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 9 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 31 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 1 (64 kDa, Protein from Saccharomyces cerevisiae S288C)

[Show more]

EMD-26544

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Mediator-PIC Early (Core B)

Release date: Feb. 15, 2023
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7uif
Q-score: 0.127

Gorbea Colon JJ, Palao 3rd L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K

Mol Cell (2023) 83 pp. 574- [ DOI: doi:10.1016/j.molcel.2023.01.011 Pubmed: 36731470 ]

Sample:

Dna-directed rna polymerase ii subunit rpb3 (35 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 14 (123 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription initiation factor iif subunit alpha (82 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 20 (22 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 7 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription elongation factor s-ii (34 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 4 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 22 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 18 (34 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 17 (78 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription initiation factor iif subunit beta (46 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 19 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
Transcription initiation factor iib (38 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 21 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 6 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 8 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator-pic early (Complex from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 10 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb2 (138 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 9 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 31 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase ii subunit rpb1 (191 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Mediator of rna polymerase ii transcription subunit 1 (64 kDa, Protein from Saccharomyces cerevisiae S288C)

[Show more]

EMD-26545

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Mediator-PIC Early (Mediator A)

Release date: Feb. 15, 2023
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7uig
Q-score: 0.172

Gorbea Colon JJ, Palao 3rd L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K

Mol Cell (2023) 83 pp. 574- [ DOI: doi:10.1016/j.molcel.2023.01.011 Pubmed: 36731470 ]

Sample:

Mediator of rna polymerase ii transcription subunit 6 (32 kDa, Protein from baker's yeast)
Core mediator-picearly (copy a) (Complex from Saccharomyces cerevisiae)
Mediator of rna polymerase ii transcription subunit 4 (32 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 7 (25 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 18 (34 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 20 (22 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 10 (17 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 14 (123 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 17 (78 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 21 (16 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 1 (64 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 8 (25 kDa, Protein from baker's yeast)
Dna-directed rna polymerase ii subunit rpb1 (191 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 9 (17 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 31 (14 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 19 (24 kDa, Protein from baker's yeast)
Mediator of rna polymerase ii transcription subunit 22 (13 kDa, Protein from baker's yeast)

[Show more]

EMD-34359

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PIC-Mediator in complex with +1 nucleosome (T40N) in MH-binding state

Release date: Nov. 2, 2022
Resolution: 5.04 Å
EM Method: Single-particle
Fitted PDBs: 8gxq
Q-score: 0.084

Chen X, Wang X, Liu W, Ren Y, Qu X, Li J, Yin X, Xu Y

Science (2022) 378 pp. 62-68 [ Pubmed: 36201575 DOI: doi:10.1126/science.abn8131 ]

Sample:

Dna (228-mer) (70 kDa, DNA from synthetic construct)
Mediator of rna polymerase ii transcription subunit 18 (23 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 14 (160 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa)
General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 1 (168 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 19 (26 kDa, Protein from Homo sapiens)
Iron/sulfur cluster (351 Da, Ligand)
General transcription factor iih subunit 1 (62 kDa, Protein from Homo sapiens)
Pic-mediator in complex with +1 nucleosome (t40n) in mh-binding state (Complex from Sus scrofa)
Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
Cyclin-h (37 kDa, Protein from Homo sapiens)
General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 25 (78 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 21 (15 kDa, Protein from Homo sapiens)
Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 11 (23 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 3 (103 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 4 (29 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 12 (17 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 24 (110 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 5 (86 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 27 (35 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 9 (29 kDa, Protein from Homo sapiens)
Cdk-activating kinase assembly factor mat1 (35 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 7 (40 kDa, Protein from Homo sapiens)
Dna (162-mer) (53 kDa, DNA from synthetic construct)
Dna (228-mer) (70 kDa, DNA from synthetic construct)
Mediator of rna polymerase ii transcription subunit 31 (15 kDa, Protein from Homo sapiens)
General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 30 (20 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 2 (137 kDa, Protein from Homo sapiens)
Isoform 2 of mediator of rna polymerase ii transcription subunit 16 (93 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 4 (110 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 10 (21 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 8 (34 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 9 (16 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit f (14 kDa, Protein from Sus scrofa)
General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 28 (19 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase subunit (217 kDa, Protein from Sus scrofa)
Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 13 (14 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 4 (52 kDa, Protein from Homo sapiens)
General transcription and dna repair factor iih helicase subunit xpb (89 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 20 (23 kDa, Protein from Homo sapiens)
General transcription and dna repair factor iih helicase subunit xpd (87 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 23 (156 kDa, Protein from Homo sapiens)
Dna (162-mer) (46 kDa, DNA from synthetic construct)
Histone h2a.z (13 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 29 (21 kDa, Protein from Homo sapiens)
Isoform 2 of mediator of rna polymerase ii transcription subunit 8 (29 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 17 (72 kDa, Protein from Homo sapiens)
Rna_pol_l_2 domain-containing protein (13 kDa, Protein from Sus scrofa)
Transcription initiation factor tfiid subunit 6 (72 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 7 (27 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 10 (15 kDa, Protein from Homo sapiens)
Histone h4 (11 kDa, Protein from Xenopus laevis)
Cyclin-dependent kinase 7 (39 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 1 (212 kDa, Protein from Homo sapiens)
Histone h3 (15 kDa, Protein from Xenopus laevis)
Mediator of rna polymerase ii transcription subunit 6 (28 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
Rpb12 (7 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 26 (65 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 22 (22 kDa, Protein from Homo sapiens)
Histone h2b (13 kDa, Protein from Xenopus laevis)
Dna-directed rna polymerase subunit beta (134 kDa, Protein from Sus scrofa)
Magnesium ion (24 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 15 (86 kDa, Protein from Homo sapiens)

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EMD-34360

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PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state

Release date: Nov. 2, 2022
Resolution: 4.16 Å
EM Method: Single-particle
Fitted PDBs: 8gxs
Q-score: 0.093

Chen X, Wang X, Liu W, Ren Y, Qu X, Li J, Yin X, Xu Y

Science (2022) 378 pp. 62-68 [ Pubmed: 36201575 DOI: doi:10.1126/science.abn8131 ]

Sample:

Dna (228-mer) (70 kDa, DNA from synthetic construct)
Mediator of rna polymerase ii transcription subunit 18 (23 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 14 (160 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa)
General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 1 (168 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 19 (26 kDa, Protein from Homo sapiens)
Iron/sulfur cluster (351 Da, Ligand)
General transcription factor iih subunit 1 (62 kDa, Protein from Homo sapiens)
Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
Cyclin-h (37 kDa, Protein from Homo sapiens)
General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 25 (78 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 21 (15 kDa, Protein from Homo sapiens)
Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 11 (23 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 3 (103 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 4 (29 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 12 (17 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 24 (110 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 5 (86 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 27 (35 kDa, Protein from Homo sapiens)
Cdk-activating kinase assembly factor mat1 (35 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 9 (29 kDa, Protein from Homo sapiens)
Dna (162-mer) (53 kDa, DNA from synthetic construct)
Transcription initiation factor tfiid subunit 7 (40 kDa, Protein from Homo sapiens)
Dna (228-mer) (70 kDa, DNA from synthetic construct)
Mediator of rna polymerase ii transcription subunit 31 (15 kDa, Protein from Homo sapiens)
General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 30 (20 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 2 (137 kDa, Protein from Homo sapiens)
Isoform 2 of mediator of rna polymerase ii transcription subunit 16 (93 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 4 (110 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 10 (21 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 8 (34 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 9 (16 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit f (14 kDa, Protein from Sus scrofa)
General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 28 (19 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase subunit (217 kDa, Protein from Sus scrofa)
Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 13 (14 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 4 (52 kDa, Protein from Homo sapiens)
General transcription and dna repair factor iih helicase subunit xpb (89 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 20 (23 kDa, Protein from Homo sapiens)
General transcription and dna repair factor iih helicase subunit xpd (87 kDa, Protein from Homo sapiens)
Dna (162-mer) (46 kDa, DNA from synthetic construct)
Mediator of rna polymerase ii transcription subunit 23 (156 kDa, Protein from Homo sapiens)
Histone h2a.z (13 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 29 (21 kDa, Protein from Homo sapiens)
Isoform 2 of mediator of rna polymerase ii transcription subunit 8 (29 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 17 (72 kDa, Protein from Homo sapiens)
Rna_pol_l_2 domain-containing protein (13 kDa, Protein from Sus scrofa)
Transcription initiation factor tfiid subunit 6 (72 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 7 (27 kDa, Protein from Homo sapiens)
Pic-mediator in complex with +1 nucleosome (t40n) in h-binding state (Complex from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 10 (15 kDa, Protein from Homo sapiens)
Histone h4 (11 kDa, Protein from Xenopus laevis)
Cyclin-dependent kinase 7 (39 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 1 (212 kDa, Protein from Homo sapiens)
Histone h3 (15 kDa, Protein from Xenopus laevis)
Mediator of rna polymerase ii transcription subunit 6 (28 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
Rpb12 (7 kDa, Protein from Sus scrofa)
Mediator of rna polymerase ii transcription subunit 26 (65 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 22 (22 kDa, Protein from Homo sapiens)
Histone h2b (13 kDa, Protein from Xenopus laevis)
Dna-directed rna polymerase subunit beta (134 kDa, Protein from Sus scrofa)
Magnesium ion (24 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 15 (86 kDa, Protein from Homo sapiens)

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EMD-31204

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TFIID-based PIC-Mediator holo-complex in pre-assembled state (pre-hPIC-MED)

Release date: May 26, 2021
Resolution: 4.07 Å
EM Method: Single-particle
Fitted PDBs: 7ena
Q-score: 0.168

Chen X, Yin X, Li J, Wu Z, Qi Y, Wang X, Liu W, Xu Y

Science (2021) 372 [ Pubmed: 33958484 DOI: doi:10.1126/science.abg0635 ]

Sample:

Dna-directed rna polymerase subunit beta (134 kDa, Protein from Pig)
Mediator of rna polymerase ii transcription subunit 18 (23 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
Tfiif (Complex from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Pig)
Rpb1 (217 kDa, Protein from Pig)
General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 1 (168 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 19 (26 kDa, Protein from Homo sapiens)
Iron/sulfur cluster (351 Da, Ligand)
General transcription factor iih subunit 1 (62 kDa, Protein from Homo sapiens)
Cyclin-h (37 kDa, Protein from Homo sapiens)
Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Pig)
Rpb12 (7 kDa, Protein from Pig)
General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 21 (15 kDa, Protein from Homo sapiens)
Tfiia-a (34 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 11 (23 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 3 (103 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 4 (29 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 12 (17 kDa, Protein from Homo sapiens)
Cyclin-dependent kinase 7 (39 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 24 (110 kDa, Protein from Homo sapiens)
Tfiid (Complex from Sus scrofa)
Transcription initiation factor tfiid subunit 5 (86 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 27 (35 kDa, Protein from Homo sapiens)
Cdk-activating kinase assembly factor mat1 (35 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 9 (29 kDa, Protein from Homo sapiens)
Tfiie (Complex from Homo sapiens)
Transcription initiation factor tfiid subunit 7 (40 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 31 (15 kDa, Protein from Homo sapiens)
General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Pig)
Mediator of rna polymerase ii transcription subunit 30 (20 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 2 (137 kDa, Protein from Homo sapiens)
Isoform 2 of mediator of rna polymerase ii transcription subunit 16 (93 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 4 (110 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 10 (21 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Pig)
Tfiia (Complex from Homo sapiens)
Mediator (Complex from Homo sapiens)
Transcription initiation factor tfiid subunit 8 (34 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 7 (27 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 9 (16 kDa, Protein from Homo sapiens)
Tfiih (Complex from Homo sapiens)
General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 28 (19 kDa, Protein from Homo sapiens)
Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
Dna (69-mer) (21 kDa, DNA from Homo sapiens)
Transcription initiation factor tfiid subunit 13 (14 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 4 (52 kDa, Protein from Homo sapiens)
General transcription and dna repair factor iih helicase subunit xpb (89 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 20 (23 kDa, Protein from Homo sapiens)
General transcription and dna repair factor iih helicase subunit xpd (87 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 23 (156 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 29 (21 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 14 (160 kDa, Protein from Homo sapiens)
Isoform 2 of mediator of rna polymerase ii transcription subunit 8 (29 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Dna(69-mer) (21 kDa, DNA from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 17 (72 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 6 (72 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase (Complex)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Pig)
Rpb10 (7 kDa, Protein from Sus scrofa)
Tfiib (Complex from Homo sapiens)
Tfiid-based pic-mediator holo-complex in pre-assembled state (pre-hpic-med) (Complex from Homo sapiens)
Dna (Complex)
Mediator of rna polymerase ii transcription subunit 10 (15 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 1 (212 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit f (14 kDa, Protein from Pig)
Mediator of rna polymerase ii transcription subunit 6 (28 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Pig)
Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
Rna_pol_l_2 domain-containing protein (13 kDa, Protein from Pig)
Mediator of rna polymerase ii transcription subunit 22 (22 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 26 (65 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 15 (86 kDa, Protein from Homo sapiens)

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EMD-31207

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TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED)

Release date: May 26, 2021
Resolution: 4.13 Å
EM Method: Single-particle
Fitted PDBs: 7enc
Q-score: 0.103

Chen X, Yin X, Li J, Wu Z, Qi Y, Wang X, Liu W, Xu Y

Science (2021) 372 [ Pubmed: 33958484 DOI: doi:10.1126/science.abg0635 ]

Sample:

Transcription initiation factor tfiid subunit 8 (34 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase subunit beta (134 kDa, Protein from Pig)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Pig)
Rpb10 (7 kDa, Protein from Sus scrofa)
Transcription initiation factor tfiid subunit 11 (23 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 9 (16 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 18 (23 kDa, Protein from Homo sapiens)
Cyclin-dependent kinase 7 (39 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 24 (110 kDa, Protein from Homo sapiens)
General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 28 (19 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 5 (86 kDa, Protein from Homo sapiens)
Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Pig)
Mediator of rna polymerase ii transcription subunit 27 (35 kDa, Protein from Homo sapiens)
Rpb1 (217 kDa, Protein from Sus scrofa)
Cdk-activating kinase assembly factor mat1 (35 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 9 (29 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 10 (15 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Dna (69-mer) (21 kDa, DNA from Homo sapiens)
Transcription initiation factor tfiid subunit 7 (40 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 1 (168 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
General transcription factor iih subunit 4 (52 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 19 (26 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 13 (14 kDa, Protein from Homo sapiens)
General transcription and dna repair factor iih helicase subunit xpb (89 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 20 (23 kDa, Protein from Homo sapiens)
General transcription and dna repair factor iih helicase subunit xpd (87 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 1 (212 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 31 (15 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit f (14 kDa, Protein from Pig)
Mediator of rna polymerase ii transcription subunit 23 (156 kDa, Protein from Homo sapiens)
Iron/sulfur cluster (351 Da, Ligand)
General transcription factor iih subunit 1 (62 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 6 (28 kDa, Protein from Homo sapiens)
General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
Cyclin-h (37 kDa, Protein from Homo sapiens)
Tfiid-based pic-mediator holo-complex in fully-assembled state (hpic-med) (Complex from Homo sapiens)
General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 29 (21 kDa, Protein from Homo sapiens)
Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Pig)
Mediator of rna polymerase ii transcription subunit 14 (160 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 30 (20 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Pig)
Isoform 2 of mediator of rna polymerase ii transcription subunit 8 (29 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 2 (137 kDa, Protein from Homo sapiens)
Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 4 (110 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Pig)
Rpb12 (7 kDa, Protein from Pig)
Isoform 2 of mediator of rna polymerase ii transcription subunit 16 (93 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 17 (72 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Mediator of rna polymerase ii transcription subunit 7 (27 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 10 (21 kDa, Protein from Homo sapiens)
Rna_pol_l_2 domain-containing protein (13 kDa, Protein from Pig)
Mediator of rna polymerase ii transcription subunit 22 (22 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 26 (65 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Pig)
Dna (69-mer) (21 kDa, DNA from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 21 (15 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 6 (72 kDa, Protein from Homo sapiens)
Tfiia-a (34 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 3 (103 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 4 (29 kDa, Protein from Homo sapiens)
Mediator of rna polymerase ii transcription subunit 15 (86 kDa, Protein from Homo sapiens)
Transcription initiation factor tfiid subunit 12 (17 kDa, Protein from Homo sapiens)

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