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Intermediate assembly of the Large subunit from Leishmania major mitochondrial ribosome
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7am2
Q-score: 0.469
Soufari H, Waltz F, Parrot C, Durrieu-Gaillard S, Bochler A, Kuhn L, Sissler M, Hashem Y
PNAS (2020) 117 pp. 29851-29861 [ Pubmed: 33168716 DOI: doi:10.1073/pnas.2011301117 ]
- Bl20m (22 kDa, Protein from Leishmania tarentolae)
- Ml88 (22 kDa, Protein from Leishmania tarentolae)
- Rna uridylyltransferase (23 kDa, Protein from Leishmania tarentolae)
- Ml63 (18 kDa, Protein from Leishmania tarentolae)
- G domain-containing protein (51 kDa, Protein from Leishmania tarentolae)
- Ul22m (32 kDa, Protein from Leishmania tarentolae)
- L51_s25_ci-b8 domain-containing protein (31 kDa, Protein from Leishmania tarentolae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ml41 (22 kDa, Protein from Leishmania tarentolae)
- Putative ribosomal protein l11 (40 kDa, Protein from Leishmania tarentolae)
- Dead/deah box helicase-like protein (87 kDa, Protein from Leishmania tarentolae)
- Ribosomal rna (6 MDa, RNA from Leishmania tarentolae)
- Ml80 (27 kDa, Protein from Leishmania tarentolae)
- Ml99 (13 kDa, Protein from Leishmania tarentolae)
- Peptidyl-prolyl cis-trans isomerase (28 kDa, Protein from Leishmania tarentolae)
- Ml78 (33 kDa, Protein from Leishmania tarentolae)
- U6 (13 kDa, Protein from Leishmania tarentolae)
- Ul30m (45 kDa, Protein from Leishmania tarentolae)
- U3 (5 kDa, Protein from Leishmania tarentolae)
- Ml72 (56 kDa, Protein from Leishmania tarentolae)
- Ml78 (3 kDa, Protein from Leishmania tarentolae)
- U2 (2 kDa, Protein from Leishmania tarentolae)
- Ul24m (53 kDa, Protein from Leishmania tarentolae)
- G domain-containing protein (86 kDa, Protein from Leishmania tarentolae)
- Bl28m (27 kDa, Protein from Leishmania tarentolae)
- Ml95 (18 kDa, Protein from Leishmania tarentolae)
- Ribosomal protein l3-like protein (54 kDa, Protein from Leishmania tarentolae)
- Ribosomal_l18e/l15p domain-containing protein (43 kDa, Protein from Leishmania tarentolae)
- Ml68 (65 kDa, Protein from Leishmania tarentolae)
- Ml86 (21 kDa, Protein from Leishmania tarentolae)
- Ml84 (24 kDa, Protein from Leishmania tarentolae)
- Ul4m (50 kDa, Protein from Leishmania tarentolae)
- Ml59/64 (28 kDa, Protein from Leishmania tarentolae)
- Ml98 (13 kDa, Protein from Leishmania tarentolae)
- Ml42 (22 kDa, Protein from Leishmania tarentolae)
- Ml87 (22 kDa, Protein from Leishmania tarentolae)
- Bl19m (16 kDa, Protein from Leishmania tarentolae)
- Bl21m (21 kDa, Protein from Leishmania tarentolae)
- Ml75 (39 kDa, Protein from Leishmania tarentolae)
- Ml94 (17 kDa, Protein from Leishmania tarentolae)
- Ul29m (54 kDa, Protein from Leishmania tarentolae)
- Bl32m (9 kDa, Protein from Leishmania tarentolae)
- Spou_methylase domain-containing protein (63 kDa, Protein from Leishmania tarentolae)
- Ul23m (28 kDa, Protein from Leishmania tarentolae)
- Ml81 (37 kDa, Protein from Leishmania tarentolae)
- Putative ribosomal protein l14 (21 kDa, Protein from Leishmania tarentolae)
- Ml67 (97 kDa, Protein from Leishmania tarentolae)
- R1 (896 Da, RNA from Leishmania tarentolae)
- Ml52,ml52 (29 kDa, Protein from Leishmania tarentolae)
- L0r8f8 (14 kDa, Protein from Leishmania tarentolae)
- Ml49 (26 kDa, Protein from Leishmania tarentolae)
- Ml100 (146 kDa, Protein from Leishmania tarentolae)
- Acyl carrier protein (16 kDa, Protein from Leishmania tarentolae)
- Ribosomal_l9 domain-containing protein (30 kDa, Protein from Leishmania tarentolae)
- Ml53 (35 kDa, Protein from Leishmania tarentolae)
- 50s ribosomal protein l13-like protein (20 kDa, Protein from Leishmania tarentolae)
- Lim zinc-binding domain-containing protein (25 kDa, Protein from Leishmania tarentolae)
- U5 (6 kDa, Protein from Leishmania tarentolae)
- Ml93 (18 kDa, Protein from Leishmania tarentolae)
- Putative 50s ribosomal protein l17 (35 kDa, Protein from Leishmania tarentolae)
- R2 (10 kDa, RNA from Leishmania tarentolae)
- Ml85 (27 kDa, Protein from Leishmania tarentolae)
- Trud domain-containing protein (68 kDa, Protein from Leishmania tarentolae)
- R5 (1 kDa, RNA from Leishmania tarentolae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase (25 kDa, Protein from Leishmania tarentolae)
- Ml74 (39 kDa, Protein from Leishmania tarentolae)
- Ml71 (49 kDa, Protein from Leishmania tarentolae)
- Ml70 (50 kDa, Protein from Leishmania tarentolae)
- Gtpase der (56 kDa, Protein from Leishmania tarentolae)
- U4 (9 kDa, Protein from Leishmania tarentolae)
- Pseudouridylate synthase-like protein (47 kDa, Protein from Leishmania tarentolae)
- Ml89 (21 kDa, Protein from Leishmania tarentolae)
- Ua (14 kDa, Protein from Leishmania tarentolae)
- Dnaj-like protein (45 kDa, Protein from Leishmania tarentolae)
- Bl35m (18 kDa, Protein from Leishmania tarentolae)
- Ml76 (38 kDa, Protein from Leishmania tarentolae)
- U8 (4 kDa, Protein from Leishmania tarentolae)
- U1 (3 kDa, Protein from Leishmania tarentolae)
- Leishmania mitochondrial ribosome (Complex from Leishmania tarentolae)
State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6yxy
Q-score: 0.508
Jaskolowski M, Ramrath DJF, Bieri P, Niemann M, Mattei S, Calderaro S, Leibundgut M, Horn EK, Boehringer D, Schneider A, Ban N
Mol Cell (2020) 79 pp. 629-644.e4 [ Pubmed: 32679035 DOI: doi:10.1016/j.molcel.2020.06.030 ]
- Ml78 (35 kDa, Protein from Trypanosoma brucei brucei)
- Ml95 (17 kDa, Protein from Trypanosoma brucei brucei)
- Ul3m (55 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf14 (82 kDa, Protein from Trypanosoma brucei brucei)
- Ul11m (40 kDa, Protein from Trypanosoma brucei brucei)
- Ml94 (17 kDa, Protein from Trypanosoma brucei brucei)
- Ml76 (39 kDa, Protein from Trypanosoma brucei brucei)
- Ml90 (22 kDa, Protein from Trypanosoma brucei brucei)
- Ml89 (22 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf20 (6 kDa, Protein from Trypanosoma brucei brucei)
- Ml81 (29 kDa, Protein from Trypanosoma brucei brucei)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- Ul4m (53 kDa, Protein from Trypanosoma brucei brucei)
- Ul22m (32 kDa, Protein from Trypanosoma brucei brucei)
- Unk (6 kDa, Protein from Trypanosoma brucei brucei)
- Ml64 (28 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf19 (12 kDa, Protein from Trypanosoma brucei brucei)
- Mt-enga (65 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf7 (18 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf12 (77 kDa, Protein from Trypanosoma brucei brucei)
- Ul30m (25 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf2 (85 kDa, Protein from Trypanosoma brucei brucei)
- Spermidine (145 Da, Ligand)
- Ml53 (35 kDa, Protein from Trypanosoma brucei brucei)
- Bl20m (24 kDa, Protein from Trypanosoma brucei brucei)
- Ml63 (18 kDa, Protein from Trypanosoma brucei brucei)
- Ml42 (21 kDa, Protein from Trypanosoma brucei brucei)
- Bl17m (35 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf6 (40 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf18 (58 kDa, Protein from Trypanosoma brucei brucei)
- Ml41 (22 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf4 (69 kDa, Protein from Trypanosoma brucei brucei)
- Ml84 (24 kDa, Protein from Trypanosoma brucei brucei)
- Ml100 (10 kDa, Protein from Trypanosoma brucei brucei)
- Ml77 (43 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf15a (34 kDa, Protein from Trypanosoma brucei brucei)
- Ml101 (28 kDa, Protein from Trypanosoma brucei brucei)
- Ml80 (34 kDa, Protein from Trypanosoma brucei brucei)
- Bl19m (16 kDa, Protein from Trypanosoma brucei brucei)
- Unk (15 kDa, Protein from Trypanosoma brucei brucei)
- Mt-engb (71 kDa, Protein from Trypanosoma brucei brucei)
- Ul14m (25 kDa, Protein from Trypanosoma brucei brucei)
- Ml99 (12 kDa, Protein from Trypanosoma brucei brucei)
- Ml98 (12 kDa, Protein from Trypanosoma brucei brucei)
- Ml70 (62 kDa, Protein from Trypanosoma brucei brucei)
- Ml85 (22 kDa, Protein from Trypanosoma brucei brucei)
- Ml72 (49 kDa, Protein from Trypanosoma brucei brucei)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Mt-laf8 (68 kDa, Protein from Trypanosoma brucei brucei)
- Ml71 (50 kDa, Protein from Trypanosoma brucei brucei)
- Mtg1 (50 kDa, Protein from Trypanosoma brucei brucei)
- Ml92 (20 kDa, Protein from Trypanosoma brucei brucei)
- Ul24m (43 kDa, Protein from Trypanosoma brucei brucei)
- Mt-acp (16 kDa, Protein from Trypanosoma brucei brucei)
- Ul13m (24 kDa, Protein from Trypanosoma brucei brucei)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Ml43 (31 kDa, Protein from Trypanosoma brucei brucei)
- Water (18 Da, Ligand)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- State b of the trypanosoma brucei mitoribosomal large subunit assembly intermediate (Complex from Trypanosoma brucei brucei)
- Mt-laf5 (67 kDa, Protein from Trypanosoma brucei brucei)
- 12s ribosomal rna (373 kDa, RNA from Trypanosoma brucei brucei)
- Ml86 (22 kDa, Protein from Trypanosoma brucei brucei)
- Ml88 (22 kDa, Protein from Trypanosoma brucei brucei)
- Bl28m (28 kDa, Protein from Trypanosoma brucei brucei)
- Unk (698 Da, Protein from Trypanosoma brucei brucei)
- Bl21m (20 kDa, Protein from Trypanosoma brucei brucei)
- Ul23m (28 kDa, Protein from Trypanosoma brucei brucei)
- Ml67 (93 kDa, Protein from Trypanosoma brucei brucei)
- Malsu1 (39 kDa, Protein from Trypanosoma brucei brucei)
- Sodium ion (22 Da, Ligand)
- Bl9m (30 kDa, Protein from Trypanosoma brucei brucei)
- Ml93 (18 kDa, Protein from Trypanosoma brucei brucei)
- Ml52 (167 kDa, Protein from Trypanosoma brucei brucei)
- Zinc ion (65 Da, Ligand)
- Ml75 (39 kDa, Protein from Trypanosoma brucei brucei)
- Bl35m (21 kDa, Protein from Trypanosoma brucei brucei)
- Ul29m (54 kDa, Protein from Trypanosoma brucei brucei)
- Ml87 (22 kDa, Protein from Trypanosoma brucei brucei)
- L0r8f8 (13 kDa, Protein from Trypanosoma brucei brucei)
- Unk (1 kDa, Protein from Trypanosoma brucei brucei)
- Ml49 (24 kDa, Protein from Trypanosoma brucei brucei)
- Ml68 (62 kDa, Protein from Trypanosoma brucei brucei)
- Ul15m (43 kDa, Protein from Trypanosoma brucei brucei)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Mt-laf3 (46 kDa, Protein from Trypanosoma brucei brucei)
- S-(2-{[n-(2-hydroxy-4-{[hydroxy(oxido)phosphino]oxy}-3,3-dimethylbutanoyl)-beta-alanyl]amino}ethyl) decanethioate (496 Da, Ligand)
- Ml74 (39 kDa, Protein from Trypanosoma brucei brucei)
- Bl32m (9 kDa, Protein from Trypanosoma brucei brucei)
- Magnesium ion (24 Da, Ligand)
- Ml83 (25 kDa, Protein from Trypanosoma brucei brucei)