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Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 7nb8
Q-score: 0.268
Cook AD, Roberts AJ, Atherton J, Tewari R, Topf M, Moores CA
J Biol Chem (2021) 297 pp. 101063-101063 [ Pubmed: 34375637 DOI: doi:10.1016/j.jbc.2021.101063 ]
- 14 protofilament microtubule of alpha/beta-tubulin dimers, with kinesin-5 motor domain bound 1:1 to tubulin dimers. (Complex)
- Alpha/beta-tubulin (Complex from Sus scrofa)
- Tubulin beta chain (49 kDa, Protein from Pig)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kinesin-5 (Complex from Plasmodium falciparum (isolate 3D7))
- Kinesin-5 (46 kDa, Protein from Plasmodium falciparum (isolate 3D7))
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Pig)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 7nba
Q-score: 0.268
Cook AD, Roberts AJ, Atherton J, Tewari R, Topf M, Moores CA
J Biol Chem (2021) 297 pp. 101063-101063 [ Pubmed: 34375637 DOI: doi:10.1016/j.jbc.2021.101063 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Kinesin motor domain-containing protein,kinesin motor domain-containing protein (46 kDa, Protein from Plasmodium falciparum (isolate NF54))
- Tubulin beta chain (49 kDa, Protein from Pig)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 14 protofilament microtubule complexed with plasmodium falciparum kinesin-5 motor domain bound to amppnp. asymmetric unit. (Complex from Plasmodium falciparum (isolate NF54))
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Pig)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
Human kinesin-5 motor domain in the GSK-1 state bound to microtubules
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 6ta3
Q-score: 0.215
Pena A, Sweeney A, Cook AD, Topf M, Moores CA
Structure (2020) 28 pp. 450-457.e5 [ Pubmed: 32084356 DOI: doi:10.1016/j.str.2020.01.013 ]
- 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{h}-quinolin-2-one (291 Da, Ligand)
- Tubulin alpha-1b chain and tubulin beta chain (Complex from Sus scrofa)
- Kinesin-like protein kif11 (Complex from Homo sapiens)
- Kinesin-like protein kif11 (43 kDa, Protein from Homo sapiens)
- Tubulin beta chain (48 kDa, Protein from Pig)
- Tubulin alpha-1b chain (48 kDa, Protein from Pig)
- Ternary complex of human kinesin 5 and microtubules (Complex)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 5mm7
Q-score: 0.207
von Loeffelholz O, Ann Moores C
J Struct Biol (2019) [ DOI: doi:10.1016/j.jsb.2019.07.003 Pubmed: 31288039 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tubulin beta chain (Complex from Sus scrofa)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Ternary complex of alpha-beta-tubulin stabilized by taxol and decorated with ustilago maydis kinesin-5 motordomain with n-terminal extension (Complex)
- Tubulin alpha-1a chain (Complex from Sus scrofa)
- Tubulin alpha-1a chain (48 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kinesin-5 (Complex from Ustilago maydis)
- Magnesium ion (24 Da, Ligand)
- Kinesin-5 (49 kDa, Protein from Ustilago maydis)
- Taxol (853 Da, Ligand)
Ustilago maydis kinesin-5 motor domain in the AMPPNP state bound to microtubules
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5mm4
Q-score: 0.284
von Loeffelholz O, Ann Moores C
J Struct Biol (2019) [ DOI: doi:10.1016/j.jsb.2019.07.003 Pubmed: 31288039 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Kinesin-5 (42 kDa, Protein from Ustilago maydis (strain 521 / FGSC 9021))
- Taxol (853 Da, Ligand)
- Ternary complex of alpha-beta-tubulin stabilized by taxol and decorated with ustilago maydis kinesin-5 (Complex)
- Tubulin alpha-1a chain (48 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ustilago maydis kinesin-5 (Complex from Ustilago maydis (strain 521 / FGSC 9021))
- Magnesium ion (24 Da, Ligand)
- Alpha-beta-tubulin (Complex from Sus scrofa)
Bell KM, Cha HK, Sindelar CV, Cochran JC
J Biol Chem (2017) 292 pp. 14680-14694 [ DOI: doi:10.1074/jbc.M117.797662 Pubmed: 28701465 ]
Resolution: 9.3 Å
EM Method: Single-particle
Fitted PDBs: 5m5i, 5m5l, 5m5m, 5m5n, 5m5o
Q-score: 0.0885
Britto M, Goulet A, Rizvi S, von Loeffelholz O, Moores CA, Cross RA
PNAS (2016) 113 pp. E7483-E7489 [ DOI: doi:10.1073/pnas.1611581113 Pubmed: 27834216 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Microtubule-bound s.pombe kinesin-5 motor domain in the amppnp state (140 kDa, Complex)
- Tubulin beta-2b chain (49 kDa, Protein from Bos taurus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kinesin-like protein cut7 (40 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Tubulin alpha-1d chain (50 kDa, Protein from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Taxol (853 Da, Ligand)
Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in the ADP.AlFx state.
Resolution: 9.2 Å
EM Method: Single-particle
Fitted PDBs: 4ck6, 4ck7
Q-score: 0.106
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ Pubmed: 24449904 DOI: doi:10.1073/pnas.1319848111 ]
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ DOI: doi:10.1073/pnas.1319848111 Pubmed: 24449904 ]
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ DOI: doi:10.1073/pnas.1319848111 Pubmed: 24449904 ]
- 13-protofilament microtubule-bound human kinesin-5 motor domain with adp.alfx bound. a gold cluster is covalently attached to the neck linker (v365c). (Sample)
- Kinesin-5 motor domain (Protein from Homo sapiens)
- Alpha tubulin (Protein from Bos taurus)
- Beta tubulin (Protein from Bos taurus)