Do data resources managed by EMBL-EBI and our collaborators make a difference to your work? Please take 10 minutes to fill in our annual user survey, and help us make the case for why sustaining open data resources is critical for life sciences research.
Grimm C, Jungwirth S, Fischer U
To Be Published
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus)
- Water (18 Da, Ligand)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus)
- Non-template dna oligomer and template dna oligomer (Complex from Vaccinia virus)
- Template dna oligomer (21 kDa, DNA from Vaccinia virus)
- Dna-directed rna polymerase 22 kda subunit (21 kDa, Protein from Vaccinia virus)
- Mrna-capping enzyme catalytic subunit (96 kDa, Protein from Vaccinia virus)
- Zinc ion (65 Da, Ligand)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (750 kDa, Complex)
- Intermediate transcription factor 3 large subunit (44 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (Complex from Vaccinia virus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 133 kda polypeptide (133 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 small subunit (33 kDa, Protein from Vaccinia virus)
- Mrna-capping enzyme regulatory subunit opg124 (33 kDa, Protein from Vaccinia virus)
- Non-template dna oligomer (21 kDa, DNA from Vaccinia virus)
Grimm C, Jungwirth S, Fischer U
To Be Published
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus)
- Template dna oligomer (Complex from Vaccinia virus)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 133 kda polypeptide (133 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus)
- Mrna-capping enzyme catalytic subunit (96 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus)
- Template dna oligomer (21 kDa, DNA from Vaccinia virus)
- Non-template dna oligomer (21 kDa, DNA from Vaccinia virus)
- Zinc ion (65 Da, Ligand)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (750 kDa, Complex)
- Dna-directed rna polymerase 22 kda subunit (21 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 small subunit (33 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 large subunit (44 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (Complex from Vaccinia virus)
Grimm C, Jungwirth S, Fischer U
To Be Published
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 133 kda polypeptide (133 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus)
- Mrna-capping enzyme catalytic subunit (96 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus)
- Mrna-capping enzyme regulatory subunit opg124 (33 kDa, Protein from Vaccinia virus)
- Template dna oligomer (21 kDa, DNA from Vaccinia virus)
- Non-template dna oligomer (21 kDa, DNA from Vaccinia virus)
- Zinc ion (65 Da, Ligand)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (750 kDa, Complex from Vaccinia virus)
- Dna-directed rna polymerase 22 kda subunit (21 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 small subunit (33 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 large subunit (44 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus)
Cryo-EM structure of Pyrococcus furiosus transcription elongation complex bound to Spt4/5
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 8oki
Q-score: 0.48
Tarau D, Grunberger F, Pilsl M, Reichelt R, Heiss F, Konig S, Urlaub H, Hausner W, Engel C, Grohmann D
Nucleic Acids Res (2024) [ Pubmed: 38709902 DOI: doi:10.1093/nar/gkae282 ]
- Dna-directed rna polymerase subunit rpo6 (6 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo3 (31 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo10 (7 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo7 (21 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo5 (9 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit beta (127 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Nucleotide strands (Complex from Pyrococcus furiosus DSM 3638)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit rpo12 (5 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Transcription elongation complex with spt4/5 (Complex)
- Dna-directed rna polymerase subunit rpo4 (14 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Pyrococcus furiosus rna polymerase (Complex from Pyrococcus furiosus DSM 3638)
- Rna (2 kDa, RNA from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo11 (11 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo1n (103 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna non-template strand (7 kDa, DNA from Pyrococcus furiosus DSM 3638)
- Dna template strand (8 kDa, DNA from Pyrococcus furiosus DSM 3638)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit rpo1c (44 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Transcription elongation factor spt5 (16 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Spt4/5 (Complex from Pyrococcus furiosus DSM 3638)
- Transcription elongation factor spt4 (6 kDa, Protein from Pyrococcus furiosus DSM 3638)
Cryo-EM structure of Pyrococcus furiosus apo form RNA polymerase open clamp conformation
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8orq
Q-score: 0.477
Tarau D, Grunberger F, Pilsl M, Reichelt R, Heiss F, Konig S, Urlaub H, Hausner W, Engel C, Grohmann D
Nucleic Acids Res (2024) [ Pubmed: 38709902 DOI: doi:10.1093/nar/gkae282 ]
- Dna-directed rna polymerase subunit rpo6 (6 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo3 (31 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo1n (103 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo4 (14 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo10 (7 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo7 (21 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Pyrococcus furiosus apo form of rna polymerase open clamp conformation (Complex from Pyrococcus furiosus DSM 3638)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit rpo5 (9 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit beta (127 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit rpo11 (11 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo12 (5 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo1c (44 kDa, Protein from Pyrococcus furiosus DSM 3638)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8p2i
Q-score: 0.461
Tarau D, Grunberger F, Pilsl M, Reichelt R, Heiss F, Konig S, Urlaub H, Hausner W, Engel C, Grohmann D
Nucleic Acids Res (2024) [ Pubmed: 38709902 DOI: doi:10.1093/nar/gkae282 ]
- Dna-directed rna polymerase subunit rpo6 (6 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo3 (31 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo1n (103 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo4 (14 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo10 (7 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Transcription elongation factor spt5 (16 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo7 (21 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Pyrococcus furiosus apo form of rna polymerase open clamp conformation (Complex from Pyrococcus furiosus DSM 3638)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit rpo5 (9 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit beta (127 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Transcription elongation factor spt4 (6 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit rpo11 (11 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo12 (5 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo1c (44 kDa, Protein from Pyrococcus furiosus DSM 3638)
E. coli RNA polymerase paused at ops site
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8pdy
Q-score: 0.409
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ Pubmed: 38589445 DOI: doi:10.1038/s41467-024-47368-4 ]
- Rna (5'-r(p*up*gp*gp*cp*gp*gp*up*ap*gp*cp*gp*u)-3') (5 kDa, RNA from Escherichia coli)
- Transcription elongation complex paused at ops site, assembled on fully complementary nucleic acid scaffold (399 kDa, Complex)
- Transcription complex (Complex from Escherichia coli)
- Dna and rna (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Non-template dna (35-mer) (12 kDa, DNA from Escherichia coli)
- Template dna (35-mer) (12 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8pen
Q-score: 0.451
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ Pubmed: 38589445 DOI: doi:10.1038/s41467-024-47368-4 ]
- Transcription complex paused at ops site with fully recruited rfah (417 kDa, Complex)
- Transcription complex (Complex from Escherichia coli)
- Rna (5 kDa, RNA from Escherichia coli)
- Dna and rna (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Non-template dna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Template dna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Transcription antitermination protein rfah (18 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8pfg
Q-score: 0.471
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ Pubmed: 38589445 DOI: doi:10.1038/s41467-024-47368-4 ]
- Transcription complex (Complex from Escherichia coli)
- Dna and rna (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Template dna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Non-template dna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Transcription complex paused at ops site, assembled on not fully complementary nucleic acid scaffold, bound to disulfide-bridged (autoinhibited) rfah (417 kDa, Complex)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Rna (5 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Transcription antitermination protein rfah (18 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8pfj
Q-score: 0.434
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ Pubmed: 38589445 DOI: doi:10.1038/s41467-024-47368-4 ]
- Transcription complex (Complex from Escherichia coli)
- Transcription complex paused at ops site, bound to fully activated rfah, assembled on not fully complementary nucleic acid scaffold (417 kDa, Complex)
- Dna and rna (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Non-template dna (12 kDa, DNA from Escherichia coli)
- Template dna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Rna (5 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Transcription antitermination protein rfah (18 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)