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Cryo-EM structure of GeoCas9-sgRNA-dsDNA ternary complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8jtj
Q-score: 0.421
Shen PP, Liu BB, Li X, Zhang LL, Chen C-C, Guo R-T
To Be Published
- Dna (29-mer) (8 kDa, DNA from Geobacillus stearothermophilus)
- Rna (139-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
- Dna (5'-d(p*gp*gp*gp*cp*gp*cp*gp*ap*a)-3') (2 kDa, DNA from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
Cryo-EM structure of GeoCas9-sgRNA binary complex
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 8jtr
Q-score: 0.401
Shen PP, Liu BB, Li X, Zhang LL, Chen C-C, Guo R-T
To Be Published
- Sgrna (139-bp) (44 kDa, RNA from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
type I-B Cascade bound to a PAM-containing dsDNA target at 3.8 angstrom resolution.
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8h67
Q-score: 0.307
Lu M, Yu C, Zhang Y, Ju W, Ye Z, Hua C, Mao J, Hu C, Yang Z, Xiao Y
Nat Commun (2024) 15 pp. 4126-4126 [ DOI: doi:10.1038/s41467-024-48598-2 Pubmed: 38750051 ]
- Crispr associated protein cas7 (33 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr associated protein cas5 (26 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr rna (22 kDa, RNA from synthetic construct)
- Non target dna (2 kDa, DNA from synthetic construct)
- Cryo-em structure of synechocystis sp. pcc6714 cascade bound to a pam-containing dsdna target at 3.8 angstrom resolution. (Complex from Synechocystis sp. PCC 6714)
- Crispr associated protein cas8 (69 kDa, Protein from Synechocystis sp. PCC 6714)
- Target dna (3 kDa, DNA from synthetic construct)
- Crispr associated protein cas11b (14 kDa, Protein from Synechocystis sp. PCC 6714)
CRISPR-Cas type III-D effector complex
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8s9t
Q-score: 0.623
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a target RNA
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8s9u
Q-score: 0.584
Schwartz EA, Bravo JPK, Ahsan F, Macias LA, Brodbelt JS, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
To Be Published
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex bound to target rna (351 kDa, Complex from Synechocystis sp. PCC 6803)
- Target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a self-target RNA in the pre-cleavage state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8s9v
Q-score: 0.561
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Self-target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Water (18 Da, Ligand)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Magnesium ion (24 Da, Ligand)
CRISPR-Cas type III-D effector complex bound to self-target RNA in a post-cleavage state
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 8s9x
Q-score: 0.456
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Self-target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
Cryo-EM structure of Synechocystis sp. PCC6714 Cascade at 3.8 angstrom resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8h7q
Q-score: 0.346
To Be Published
- Crispr associated protein cas5 (26 kDa, Protein from Synechocystis sp. PCC 6714)
- Non target dna (3 kDa, DNA from synthetic construct)
- Type i-b cascade (Complex from Synechocystis sp. PCC 6714)
- Crispr rna (11 kDa, RNA from synthetic construct)
- Target dna (10 kDa, DNA from synthetic construct)
- Crispr associated protein cas8 (33 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr associated protein cas11b (14 kDa, Protein from Synechocystis sp. PCC 6714)
Cryo-EM structure of guide RNA and target RNA bound Cas7-11
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 8d1v
Q-score: 0.547
Goswami HN, Rai J, Das A, Li H
eLife (2022) 11 [ DOI: doi:10.7554/eLife.81678 Pubmed: 36190192 ]
- Cryoem structure of type iii-e cas7-11 from desulfonema ishimotonii (Complex from Desulfonema ishimotonii)
- Crispr rna (34-mer) (10 kDa, RNA from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
- Ss target rna (5'-r(p*ap*gp*cp*up*up*gp*gp*up*up*cp*ap*ap*ap*gp*ap*ap*cp*g)-3') (5 kDa, RNA from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
Staphylococcus epidermidis RP62a CRISPR effector subcomplex with non-self target RNA bound
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 7uzx
Q-score: 0.371
Smith EM, Ferrell S, Tokars VL, Mondragon A
Structure (2022) 30 pp. 1109-1128.e6 [ DOI: doi:10.1016/j.str.2022.05.013 Pubmed: 35714601 ]
- Crispr system cms endoribonuclease csm3 (24 kDa, Protein from Staphylococcus epidermidis RP62A)
- Staphylococcus epidermidis rp62a crispr rna: repeat plus spacer sequence 2 (11 kDa, RNA from Staphylococcus epidermidis RP62A)
- Crispr system cms protein csm4 (34 kDa, Protein from Staphylococcus epidermidis RP62A)
- Staphylococcus epidermidis rp62a crispr effector subcomplex with target rna bound (Complex from Staphylococcus epidermidis)
- Crispr non-self rna target (12 kDa, RNA from Staphylococcus epidermidis RP62A)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (87 kDa, Protein from Staphylococcus epidermidis RP62A)