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cryo-EM structure of a human activated spliceosome (mature Bact) at 5.1 angstrom.
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 5z56
Q-score: 0.131
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Human activated spliceosome (Complex from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Ring finger protein 113a (38 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Magnesium ion (24 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
Cryo-EM structure of the human activated spliceosome (late Bact) at 6.5 angstrom
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 5z57
Q-score: 0.097
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Late bact splieosome (Complex from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Alanine (89 Da, Ligand)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Magnesium ion (24 Da, Ligand)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 5z58
Q-score: 0.131
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Ring finger protein 113a (38 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Early bact spliceosome (Complex from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Magnesium ion (24 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
human Bact spliceosome core structure
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6ff4
Q-score: 0.443
Haselbach D, Komarov I, Agafonov DE, Hartmuth K, Graf B, Dybkov O, Urlaub H, Kastner B, Luhrmann R, Stark H
Cell (2018) 172 pp. 454-464.e11 [ Pubmed: 29361316 DOI: doi:10.1016/j.cell.2018.01.010 ]
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Inositol hexakisphosphate (660 Da, Ligand)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Pre mrna (153 kDa, RNA from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Ring finger protein 113a (38 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U5 snrna (36 kDa, RNA from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Human bact spliceosome state 1 unmasked (4 MDa, Complex from Homo sapiens)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)