Electron Microscopy Data Bank

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sample_name:"70S ribosome" AND xref_links:"uniprot" AND author_orcid:"0000-0003-2256-693x"

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EMD-12693

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Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan

Release date: Sept. 1, 2021
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7o19
Q-score: 0.555

van der Stel AX, Gordon ER, Sengupta A, Martinez AK, Klepacki D, Perry TN, Herrero Del Valle A, Vazquez-Laslop N, Sachs MS, Cruz-Vera LR, Innis CA

Nat Commun (2021) 12 pp. 5340-5340 [ DOI: doi:10.1038/s41467-021-25663-8 Pubmed: 34504068 ]

Sample:

30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
30s subunit (Complex from Escherichia coli K-12)
Tryptophan (204 Da, Ligand)
Water (18 Da, Ligand)
Ribosomal rna 23s (939 kDa, RNA from Escherichia coli K-12)
50s subunit (Complex from Escherichia coli K-12)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
Ribosomal rna 5s (38 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
P-site trna-pro (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
P-site trna-pro - tnac nascent chain (Complex from Escherichia coli K-12)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
Tryptophanase leader peptide (2 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
Mrna (Complex from Escherichia coli K-12)
70s ribosome stalled during translation of tnac with pro24 in the p-site. (Complex from Escherichia coli K-12)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli K-12)
Mrna (2 kDa, RNA from Escherichia coli K-12)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
Ribosomal rna 16s (497 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)

[Show more]

EMD-12694

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Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome complex stalled in response to L-tryptophan

Release date: Sept. 1, 2021
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7o1a
Q-score: 0.573

van der Stel AX, Gordon ER, Sengupta A, Martinez AK, Klepacki D, Perry TN, Herrero Del Valle A, Vazquez-Laslop N, Sachs MS, Cruz-Vera LR, Innis CA

Nat Commun (2021) 12 pp. 5340-5340 [ DOI: doi:10.1038/s41467-021-25663-8 Pubmed: 34504068 ]

Sample:

50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
30s subunit (Complex from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
Tryptophan (204 Da, Ligand)
Mrna (2 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli (strain K12))
Water (18 Da, Ligand)
Ribosomal rna 23s (939 kDa, RNA from Escherichia coli K-12)
50s subunit (Complex from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
Ribosomal rna 5s (38 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
Potassium ion (39 Da, Ligand)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
P-site trna-pro - tnac-(r23f) nascent chain (Complex from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
Mrna (Complex from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
Tnac - tryptophanase leader peptide - r23f (2 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
P-site trna-pro (24 kDa, RNA from Escherichia coli (strain K12))
Zinc ion (65 Da, Ligand)
Ribosomal rna 16s (497 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
70s ribosome stalled during translation of tnac(r23f) with pro24 in the p-site. (Complex from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))

[Show more]

EMD-12695

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Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome-RF2 complex stalled in response to L-tryptophan

Release date: Sept. 1, 2021
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7o1c
Q-score: 0.555

van der Stel AX, Gordon ER, Sengupta A, Martinez AK, Klepacki D, Perry TN, Herrero Del Valle A, Vazquez-Laslop N, Sachs MS, Cruz-Vera LR, Innis CA

Nat Commun (2021) 12 pp. 5340-5340 [ DOI: doi:10.1038/s41467-021-25663-8 Pubmed: 34504068 ]

Sample:

50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
30s subunit (Complex from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
Tryptophan (204 Da, Ligand)
Water (18 Da, Ligand)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli (strain K12))
Ribosomal rna 23s (939 kDa, RNA from Escherichia coli K-12)
50s subunit (Complex from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
Ribosomal rna 5s (38 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
Potassium ion (39 Da, Ligand)
Mrna (3 kDa, RNA from Escherichia coli K-12)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
P-site trna-pro - tnac-(r23f) nascent chain (Complex from Escherichia coli K-12)
P-site trna-pro (24 kDa, RNA from Escherichia coli K-12)
Tnac-(r23f) - tryptophanase leader peptide (2 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli (strain K12))
Peptide chain release factor rf2 (41 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
Mrna (Complex from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
Zinc ion (65 Da, Ligand)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
Ribosomal rna 16s (497 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
70s ribosome stalled during translation of tnac(r23f) with pro24 in the p-site and release factor 2 in the a-site. (Complex from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
Peptide chain release factor rf2 (Complex from Escherichia coli K-12)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))

[Show more]

EMD-21857

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70S ribosome without free 5S rRNA and with a perturbed PTC

Release date: June 24, 2020
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6wnv
Q-score: 0.383

Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vazquez-Laslop N, Korostelev AA, Mankin AS

Nat Commun (2020) 11 pp. 2900-2900 [ DOI: doi:10.1038/s41467-020-16694-8 Pubmed: 32518240 ]

Sample:

50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
70s ribosome without free 5s rrna and with perturbed ptc (2 MDa, Complex from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l10 (14 kDa, Protein from Escherichia coli)
23s-5s joint ribosomal rna (982 kDa, RNA from Escherichia coli)
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)

[Show more]

EMD-21858

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Active 70S ribosome without free 5S rRNA and bound with A- and P- tRNA

Release date: June 24, 2020
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6wnw
Q-score: 0.43

Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vazquez-Laslop N, Korostelev AA, Mankin AS

Nat Commun (2020) 11 pp. 2900-2900 [ DOI: doi:10.1038/s41467-020-16694-8 Pubmed: 32518240 ]

Sample:

50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
Trnaphe (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
Trnafmet (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Active 70s ribosome without free 5s rrna and bound with a- and p- trna (2 MDa, Complex from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
Mrna (3 kDa, RNA from Escherichia coli)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l10 (14 kDa, Protein from Escherichia coli)
23s-5s joint ribosomal rna (982 kDa, RNA from Escherichia coli)
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)

[Show more]

EMD-3730

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Cryo-EM structure of the proline-rich antimicrobial peptide Api137 bound to the terminating ribosome

Release date: July 26, 2017
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5o2r
Q-score: 0.476

Florin T, Maracci C, Graf M, Karki P, Klepacki D, Berninghausen O, Beckmann R, Vazquez-Laslop N, Wilson DN, Rodnina MV, Mankin AS

Nat Struct Mol Biol (2017) 24 pp. 752-757 [ DOI: doi:10.1038/nsmb.3439 Pubmed: 28741611 ]

Sample:

50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
Escherichia coli 70s ribosome (Complex from Escherichia coli (strain K12))
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
Escherichia coli 70s ribosome (Complex)
5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli (strain K12))
Apidaecin (1 kDa, Protein from Apis mellifera)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
P-site ile-trna (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
Mrna (Complex from Staphylococcus aureus)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
Apidaecin (Complex from Apis mellifera)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli (strain K12))
Peptide chain release factor rf1 (26 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
16s ribosomal rna (498 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l10 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
Mrna (2 kDa, RNA from Staphylococcus aureus)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))

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