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Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8v9j
Q-score: 0.525
Helena-Bueno K, Rybak MY, Ekemezie CL, Sullivan R, Brown CR, Dingwall C, Basle A, Schneider C, Connolly JPR, Blaza JN, Csorgo B, Moynihan PJ, Gagnon MG, Hill CH, Melnikov SV
Nature (2024) 626 pp. 1125-1132 [ Pubmed: 38355796 DOI: doi:10.1038/s41586-024-07041-8 ]
- 30s ribosomal protein s20 (9 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 5s ribosomal rna (38 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s9 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l23 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 16s ribosomal rna (495 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l32 (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s16 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l37 (2 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Cryo-em structure of the mycobacterium smegmatis 70s ribosome in complex with hibernation factor msmeg1130 (balon) (structure 4) (2 MDa, Complex from Mycolicibacterium smegmatis MC2 155)
- Poly-u mrna (873 Da, RNA from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l16 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l31 (8 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l35 (7 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s4 (23 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s10 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s6 (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l17 (21 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l13 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l19 (12 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l28 (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 23s ribosomal rna (1 MDa, RNA from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l6 (19 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l24 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l4 (22 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s18 (9 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l36 (4 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s22 (4 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s8 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l3 (23 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l33 (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s15 (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s12 (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s17 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s3 (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s11 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l14 (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l18 (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l10 (18 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s2 (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l5 (21 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s13 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l2 (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l21 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s7 (17 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l01 (25 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l9 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l30 (7 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Zinc ion (65 Da, Ligand)
- Ribosome hibernation factor balon (msmeg_1130) (41 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s14 (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l20 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Pe/e deacylated phenylanaline-trna (24 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l27 (9 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l22 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l15 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s19 (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l11 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s5 (21 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l25 (22 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l34 (5 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l29 (8 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8v9l
Q-score: 0.527
Helena-Bueno K, Rybak MY, Ekemezie CL, Sullivan R, Brown CR, Dingwall C, Basle A, Schneider C, Connolly JPR, Blaza JN, Csorgo B, Moynihan PJ, Gagnon MG, Hill CH, Melnikov SV
Nature (2024) 626 pp. 1125-1132 [ Pubmed: 38355796 DOI: doi:10.1038/s41586-024-07041-8 ]
- Elongation factor tu (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s20 (9 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 5s ribosomal rna (38 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s9 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l32 (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 16s ribosomal rna (495 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s16 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl33a (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l35 (7 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s4 (23 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s10 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s6 (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul23 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Small ribosomal subunit protein bs18b (9 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l17 (21 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l19 (12 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 23s ribosomal rna (1 MDa, RNA from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l6 (19 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l24 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl20 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l36 (4 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s22 (4 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Cryo-em structure of the mycobacterium smegmatis 70s ribosome in complex with hibernation factor msmeg1130 (balon) and msmegef-tu(gdp) (composite structure 6) (2 MDa, Complex from Mycolicibacterium smegmatis MC2 155)
- Small ribosomal subunit protein us5 (21 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l3 (23 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s12 (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s17 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s3 (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s11 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l14 (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l10 (18 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s2 (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s13 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l2 (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s7 (17 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l9 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l30 (7 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Zinc ion (65 Da, Ligand)
- Ribosome hibernation factor balon (msmeg_1130) (41 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Pe/e deacylated phenylanaline-trna (24 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl31 (8 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l27 (9 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l15 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Poly-u mrna (1 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l27 (2 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s19 (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l11 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul18 (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul22 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l25 (22 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Small ribosomal subunit protein us14b (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul5 (21 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l34 (5 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l29 (8 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Helena-Bueno K, Rybak MY, Ekemezie CL, Sullivan R, Brown CR, Dingwall C, Basle A, Schneider C, Connolly JPR, Blaza JN, Csorgo B, Moynihan PJ, Gagnon MG, Hill CH, Melnikov SV
Nature (2024) 626 pp. 1125-1132 [ Pubmed: 38355796 DOI: doi:10.1038/s41586-024-07041-8 ]
Helena-Bueno K, Rybak MY, Ekemezie CL, Sullivan R, Brown CR, Dingwall C, Basle A, Schneider C, Connolly JPR, Blaza JN, Csorgo B, Moynihan PJ, Gagnon MG, Hill CH, Melnikov SV
Nature (2024) 626 pp. 1125-1132 [ Pubmed: 38355796 DOI: doi:10.1038/s41586-024-07041-8 ]