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proteasome* AND xref_links:"pfam" AND release_date:[2020-01-01T00:00:00Z TO 2020-12-31T23:59:59Z] AND film_detector_model:"FEI FALCON II (4K X 4K)"

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EMD-11360

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Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Post-A1

Release date: Sept. 23, 2020
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6zqd
Q-score: 0.387

Cheng J, Lau B, La Venuta G, Ameismeier M, Berninghausen O, Hurt E, Beckmann R

Science (2020) 369 pp. 1470-1476 [ Pubmed: 32943521 DOI: doi:10.1126/science.abb4119 ]

Sample:

Bud site selection protein 21 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 10 (200 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rrna-processing protein fcf2 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Protein sof1 (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleolar protein 58 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 13 (91 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleolar complex protein 4 (63 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Something about silencing protein 10 (70 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
90s pre-ribbosome state post-a1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 7 (62 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Guanosine-5'-triphosphate (523 Da, Ligand)
40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 9 (65 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 snorna (106 kDa, RNA from Saccharomyces cerevisiae S288C)
U3 small nucleolar rna-associated protein 21 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosomal rna-processing protein 7 (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
18s rrna (580 kDa, RNA from Saccharomyces cerevisiae S288C)
Periodic tryptophan protein 2 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosomal rna-processing protein 9 (65 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 8 (80 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
5ets rna (26 kDa, RNA from Saccharomyces cerevisiae S288C)
Ribosomal rna small subunit methyltransferase nep1 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-rrna-processing protein pno1 (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein mpp10 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 5 (72 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rps5p (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 15 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 20 (287 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 6 (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 22 (140 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rrna biogenesis protein rrp5 (193 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Net1-associated nuclear protein 1 (101 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dimethyladenosine transferase (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein bms1 (135 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 12 (106 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 11 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleolar complex protein 14 (94 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 18 (66 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 4 (87 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U3 small nucleolar rna-associated protein 14 (103 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rrna 2'-o-methyltransferase fibrillarin (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rrna-processing protein fcf1 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleolar protein 56 (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Essential nuclear protein 1 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Probable atp-dependent rna helicase dhr1 (145 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Magnesium ion (24 Da, Ligand)
40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

[Show more]

EMD-10841

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pre-60S State NE1 (TAP-Flag-Nop53)

Release date: July 29, 2020
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6ylx
Q-score: 0.384

Kater L, Mitterer V, Thoms M, Cheng J, Berninghausen O, Beckmann R, Hurt E

Mol Cell (2020) 79 pp. 615-628.e5 [ Pubmed: 32668200 DOI: doi:10.1016/j.molcel.2020.06.032 ]

Sample:

60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
Its2 (74 kDa, RNA from Baker's yeast)
60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
5.8s rrna (50 kDa, RNA from Baker's yeast)
60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein 15 (25 kDa, Protein from Baker's yeast)
25s rrna (1 MDa, RNA from Baker's yeast)
60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l14-a (15 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein rlp24 (24 kDa, Protein from Baker's yeast)
Pre-60s assembly state ne1 (Complex from Saccharomyces cerevisiae)
Ribosome assembly factor mrt4 (27 kDa, Protein from Baker's yeast)
Nuclear gtp-binding protein nug1 (57 kDa, Protein from Baker's yeast)
60s ribosomal protein l13-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
Nucleolar gtp-binding protein 1 (74 kDa, Protein from Baker's yeast)
60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
Eukaryotic translation initiation factor 6 (26 kDa, Protein from Baker's yeast)
60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
60s ribosomal protein l8-a (28 kDa, Protein from Baker's yeast)
60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein nsa2 (29 kDa, Protein from Baker's yeast)
Upf0642 protein ybl028c (12 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein rlp7 (36 kDa, Protein from Baker's yeast)
60s ribosomal protein l30 (11 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein nop53 (52 kDa, Protein from Baker's yeast)
60s ribosomal protein l22-a (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l34-a (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l7-a (27 kDa, Protein from Baker's yeast)
60s ribosomal protein l25 (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l6-a (20 kDa, Protein from Baker's yeast)
27s pre-rrna (guanosine(2922)-2'-o)-methyltransferase (96 kDa, Protein from Baker's yeast)
60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)
60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
Pescadillo homolog (69 kDa, Protein from Baker's yeast)
60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
Proteasome-interacting protein cic1 (42 kDa, Protein from Baker's yeast)

[Show more]

EMD-10842

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pre-60S State NE2 (TAP-Flag-Nop53)

Release date: July 29, 2020
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6yly
Q-score: 0.392

Kater L, Mitterer V, Thoms M, Cheng J, Berninghausen O, Beckmann R, Hurt E

Mol Cell (2020) 79 pp. 615-628.e5 [ Pubmed: 32668200 DOI: doi:10.1016/j.molcel.2020.06.032 ]

Sample:

60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
Its2 (74 kDa, RNA from Baker's yeast)
60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
5.8s rrna (50 kDa, RNA from Baker's yeast)
60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
Bud site selection protein 20 (18 kDa, Protein from Baker's yeast)
60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein 15 (25 kDa, Protein from Baker's yeast)
25s rrna (1 MDa, RNA from Baker's yeast)
60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l14-a (15 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein rlp24 (24 kDa, Protein from Baker's yeast)
60s ribosomal protein l2-a (27 kDa, Protein from Baker's yeast)
Ribosome assembly factor mrt4 (27 kDa, Protein from Baker's yeast)
Nuclear gtp-binding protein nug1 (57 kDa, Protein from Baker's yeast)
60s ribosomal protein l13-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
Nucleolar gtp-binding protein 1 (74 kDa, Protein from Baker's yeast)
60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
Eukaryotic translation initiation factor 6 (26 kDa, Protein from Baker's yeast)
60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
60s ribosomal protein l8-a (28 kDa, Protein from Baker's yeast)
60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein nsa2 (29 kDa, Protein from Baker's yeast)
Upf0642 protein ybl028c (12 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein rlp7 (36 kDa, Protein from Baker's yeast)
60s ribosomal protein l30 (11 kDa, Protein from Baker's yeast)
Ribosome biogenesis protein nop53 (52 kDa, Protein from Baker's yeast)
60s ribosomal protein l22-a (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l43-a (10 kDa, Protein from Baker's yeast)
60s ribosomal protein l34-a (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
60s ribosomal protein l7-a (27 kDa, Protein from Baker's yeast)
60s ribosomal protein l25 (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l6-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)
60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
Pescadillo homolog (69 kDa, Protein from Baker's yeast)
60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
Proteasome-interacting protein cic1 (42 kDa, Protein from Baker's yeast)
Pre-60s assembly state ne2 (Complex from Saccharomyces cerevisiae)

[Show more]

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EMDB [3]

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