Do data resources managed by EMBL-EBI and our collaborators make a difference to your work? Please take 10 minutes to fill in our annual user survey, and help us make the case for why sustaining open data resources is critical for life sciences research. We appreciate your responses prior to closing of the survey on Monday 17th June.
State E2 nucleolar 60S ribosomal biogenesis intermediate - Overall map
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7nac
Q-score: 0.549
Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP
Nat Struct Mol Biol (2022) 29 pp. 1228-1238 [ DOI: doi:10.1038/s41594-022-00874-9 Pubmed: 36482249 ]
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosome subunit biogenesis protein nip7 (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein ebp2 (49 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (91 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Noc2 isoform 1 (81 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rrna helicase spb4 (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal protein (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s ribosomal rna gene and internal transcribed spacer 2 (27 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex-associated protein 3 (75 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (cytosine(2870)-c(5))-methyltransferase (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrp17 isoform 1 (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on ytm1 and spb4. (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- 27s pre-rrna (guanosine(2922)-2'-o)-methyltransferase (96 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ytm1 isoform 1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal subunit assembly/export protein loc1 (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar protein 16 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
State E1 nucleolar 60S ribosome biogenesis intermediate - Composite model
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7r7a
Q-score: 0.527
Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP
Nat Struct Mol Biol (2022) 29 pp. 1228-1238 [ DOI: doi:10.1038/s41594-022-00874-9 Pubmed: 36482249 ]
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosome subunit biogenesis protein nip7 (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein ebp2 (49 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (91 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex protein 2 (81 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rrna helicase spb4 (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal protein (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s ribosomal rna gene and internal transcribed spacer 2 (27 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex-associated protein 3 (75 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (cytosine(2870)-c(5))-methyltransferase (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on ytm1 and spb4. (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 27s pre-rrna (guanosine(2922)-2'-o)-methyltransferase (96 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ytm1 isoform 1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal subunit assembly/export protein loc1 (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar protein 16 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)