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3D Reconstruction of PPG Matrix-Landed 20S Proteasome Core Particle Protein Complexes
Salome AZ, Lee KW, Grant T, Westphall MS, Coon JJ
Anal Chem (2022) 94 pp. 17616-17624 [ Pubmed: 36475605 DOI: doi:10.1021/acs.analchem.2c04263 ]
Cryo-EM structure of the 90S small subunit pre-ribosome (Dhr1-depleted, Enp1-TAP, state 1)
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 5wyj
Q-score: 0.16
Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K
eLife (2017) 6 [ Pubmed: 28244370 DOI: doi:10.7554/eLife.22086 ]
- 5ets rna (225 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- Krr1 small subunit processome component (37 kDa, Protein from Baker's yeast)
- Mpp10,u3 small nucleolar rna-associated protein mpp10 (54 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 12 (106 kDa, Protein from Baker's yeast)
- Utp4 (66 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- 90s small subunit pre-ribosome (dhr1-depleted, enp1-tap) (Complex from Saccharomyces cerevisiae)
- Utp5 (54 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Baker's yeast)
- Nucleolar protein 56 (56 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 13 (91 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein utp30 (31 kDa, Protein from Baker's yeast)
- U3 rna (106 kDa, RNA from Baker's yeast)
- Utp8 (60 kDa, Protein from Baker's yeast)
- 18s ribosomal rna (583 kDa, RNA from Baker's yeast)
- Utp7 (47 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 7 (34 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- Utp9 (48 kDa, Protein from Baker's yeast)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Baker's yeast)
- Utp15 (43 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 9 (65 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- Utp17 (76 kDa, Protein from Baker's yeast)
- Helical domain protein (137 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein bms1 (135 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 21 (104 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s12 (15 kDa, Protein from Baker's yeast)
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 10,utp10 (173 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein pno1 (30 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 18 (66 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- Periodic tryptophan protein 2 (104 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from Baker's yeast)
- Essential nuclear protein 1 (55 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Baker's yeast)
- Sof1 (41 kDa, Protein from Baker's yeast)
- Rrna biogenesis protein rrp5 (193 kDa, Protein from Baker's yeast)
- Unassigned helices (87 kDa, Protein from Baker's yeast)
- Ribosomal rna small subunit methyltransferase nep1 (27 kDa, Protein from Baker's yeast)
- Helical domain protein (84 kDa, Protein from Baker's yeast)
- Enp2 (60 kDa, Protein from Baker's yeast)
- Utp20 (212 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 22 (140 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- Nucleolar protein 58 (57 kDa, Protein from Baker's yeast)
- Rrna 2'-o-methyltransferase fibrillarin (34 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- Utp11 (21 kDa, Protein from Baker's yeast)
- Rrna-processing protein fcf1 (21 kDa, Protein from Baker's yeast)
Cryo-EM structure of the 90S small subunit pre-ribosome (Mtr4-depleted, Enp1-TAP)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5wyk
Q-score: 0.267
Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K
eLife (2017) 6 [ Pubmed: 28244370 DOI: doi:10.7554/eLife.22086 ]
- U3 small nucleolar rna-associated protein 10 (200 kDa, Protein from Baker's yeast)
- 5ets rna (225 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- Krr1 small subunit processome component (37 kDa, Protein from Baker's yeast)
- Mpp10,u3 small nucleolar rna-associated protein mpp10 (54 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 12 (106 kDa, Protein from Baker's yeast)
- Utp4 (66 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- Utp5 (54 kDa, Protein from Baker's yeast)
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Baker's yeast)
- Nucleolar protein 56 (56 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 13 (91 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein utp30 (31 kDa, Protein from Baker's yeast)
- U3 rna (106 kDa, RNA from Baker's yeast)
- Utp8 (60 kDa, Protein from Baker's yeast)
- 18s ribosomal rna (583 kDa, RNA from Baker's yeast)
- Utp7 (47 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 7 (34 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- Utp9 (48 kDa, Protein from Baker's yeast)
- Utp15 (43 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 9 (65 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- Utp17 (76 kDa, Protein from Baker's yeast)
- Helical domain protein (137 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein bms1 (135 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 21 (104 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein pno1 (30 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 18 (66 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- Periodic tryptophan protein 2 (104 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Baker's yeast)
- Sof1 (41 kDa, Protein from Baker's yeast)
- 90s small subunit pre-ribosome (mtr4-depleted, enp1-tap) (Complex from Saccharomyces cerevisiae)
- Ribosomal rna small subunit methyltransferase nep1 (27 kDa, Protein from Baker's yeast)
- Helical domain protein (84 kDa, Protein from Baker's yeast)
- Enp2 (60 kDa, Protein from Baker's yeast)
- Unassigned helices (87 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 22 (140 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- Nucleolar protein 58 (57 kDa, Protein from Baker's yeast)
- Rrna 2'-o-methyltransferase fibrillarin (34 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- Utp11 (21 kDa, Protein from Baker's yeast)
- Rrna-processing protein fcf1 (21 kDa, Protein from Baker's yeast)
The human 26S proteasome at 3.9
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5l4g, 5l4k
Q-score: 0.329
Schweitzer A, Aufderheide A, Rudack T, Beck F, Pfeifer G, Plitzko JM, Sakata E, Schulten K, Forster F, Baumeister W
PNAS (2016) 113 pp. 7816-7821 [ DOI: doi:10.1073/pnas.1608050113 Pubmed: 27342858 ]
- Human 26s proteasome (2 MDa, Complex from Homo sapiens)
Wang RY, Kudryashev M, Li X, Egelman EH, Basler M, Cheng Y, Baker D, DiMaio F
To Be Published
- 20s proteasome (700 kDa, Protein from Thermoplasma acidophilum)
- T. acidophilum 20s proteasome (700 kDa, Sample)