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Cryo-ET dataset on lamellae of Dictyostelium discoideum cells [213 tilt-series in MRC format]
Tuijtel MW, Cruz-León S, Kreysing JP, Welsch S, Hummer G, Beck M, Turoňová B
Sci Adv (2024) 10 [ DOI: 10.1126/sciadv.adk6285 Pubmed: 38669330 ]
Image sets:
Thinner is not always better: Optimising cryo lamellae for subtomogram averaging
Tuijtel MW, Cruz-Leon S, Kreysing JP, Welsch S, Hummer G, Beck M, Turonova B
Sci Adv (2024) 10 pp. eadk6285-eadk6285 [ Pubmed: 38669330 DOI: doi:10.1126/sciadv.adk6285 ]
- Ribosome (4 MDa, Complex from Dictyostelium discoideum AX2)
Tuijtel MW, Cruz-Leon S, Kreysing JP, Welsch S, Hummer G, Beck M, Turonova B
Sci Adv (2024) 10 pp. eadk6285-eadk6285 [ Pubmed: 38669330 DOI: doi:10.1126/sciadv.adk6285 ]
- Ribosome (4 MDa, Complex from Dictyostelium discoideum AX2)
Tuijtel MW, Cruz-Leon S, Kreysing JP, Welsch S, Hummer G, Beck M, Turonova B
Sci Adv (2024) 10 pp. eadk6285-eadk6285 [ Pubmed: 38669330 DOI: doi:10.1126/sciadv.adk6285 ]
- Ribosome (4 MDa, Complex from Dictyostelium discoideum AX2)
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8pk8
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 8pk9
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 8pka
Q-score: 0.624
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Acyl carrier protein (Complex from Escherichia coli BL21(DE3))
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Cysteine desulfurase, lyr motif-containing protein 4 and iron-sulfur cluster assembly enzyme iscu (Complex from Homo sapiens)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex)
- Water (18 Da, Ligand)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
Cryo-EM structure of the inward-facing FLVCR1
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8qcs
Q-score: 0.554
Ri K, Weng TH, Claveras Cabezudo A, Josting W, Zhang Y, Bazzone A, Leong NCP, Welsch S, Doty RT, Gursu G, Lim TJY, Schmidt SL, Abkowitz JL, Hummer G, Wu D, Nguyen LN, Safarian S
Nature (2024) [ Pubmed: 38778100 DOI: doi:10.1038/s41586-024-07444-7 ]
- Flvcr1 monomer (60 kDa, Complex from Homo sapiens)
- Heme transporter flvcr1 (60 kDa, Protein from Homo sapiens)