Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7mca
Q-score: 0.441
Feng X, Noguchi Y, Barbon M, Stillman B, Speck C, Li H
Nat Commun (2021) 12 pp. 3883-3883 [ Pubmed: 34162887 DOI: doi:10.1038/s41467-021-24199-1 ]
- Dna (85-mer) (26 kDa, DNA from Saccharomyces cerevisiae)
- Dna (85-mer) (26 kDa, DNA from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Cell division control protein 6 (58 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Complex of origin replication complex with cdc6 and ars1 origin dna (470 kDa, Complex from Saccharomyces cerevisiae)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM 3D map of the S. cerevisiae origin recognition complex bound to Cdc6 and ARS1 origin DNA
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7mca
Q-score: -0.014
Feng X, Noguchi Y, Barbon M, Stillman B, Speck C, Li H
Nat Commun (2021) 12 pp. 3883-3883 [ Pubmed: 34162887 DOI: doi:10.1038/s41467-021-24199-1 ]
- Ars1 dna(85-mer) (DNA from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 6 (Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (Protein from Saccharomyces cerevisiae)
- Complex of origin replication complex with cdc6 and ars1 origin dna (470 kDa, Complex from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (Protein from Saccharomyces cerevisiae)
- Cell division control protein 6 (Protein from Saccharomyces cerevisiae)
ORC-O2WH: Human Origin Recognition Complex (ORC) with dynamic/unresolved ORC1 AAA+ domain
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7jpp
Q-score: 0.434
Jaremko MJ, On KF, Thomas DR, Stillman B, Joshua-Tor L
eLife (2020) 9 [ Pubmed: 32808929 DOI: doi:10.7554/eLife.58622 ]
- Origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 1 (44 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
- Orc-o1aaa (296 kDa, Complex from Homo sapiens)
ORC-OPEN: Human Origin Recognition Complex (ORC) in an open conformation
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7jpr
Q-score: 0.276
Jaremko MJ, On KF, Thomas DR, Stillman B, Joshua-Tor L
eLife (2020) 9 [ Pubmed: 32808929 DOI: doi:10.7554/eLife.58622 ]
- Orc-open (296 kDa, Complex from Homo sapiens)
- Origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 1 (44 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
ORC-DNA: Human Origin Recognition Complex (ORC) with DNA bound in the core
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7jps
Q-score: 0.228
Jaremko MJ, On KF, Thomas DR, Stillman B, Joshua-Tor L
eLife (2020) 9 [ Pubmed: 32808929 DOI: doi:10.7554/eLife.58622 ]
- Dna (5'-d(*ap*tp*ap*tp*ap*tp*ap*tp*ap*tp*ap*ap*t)-3') (3 kDa, DNA from Spodoptera frugiperda)
- Origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 1 (44 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- Orc-dna (296 kDa, Complex from Homo sapiens)
- Dna (5'-d(*ap*tp*tp*ap*tp*ap*tp*ap*tp*ap*tp*ap*t)-3') (3 kDa, DNA from Spodoptera frugiperda)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
ORC-O1AAA: Human Origin Recognition Complex (ORC) with dynamic/unresolved ORC2 WH
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7jpo
Q-score: 0.503
Jaremko MJ, On KF, Thomas DR, Stillman B, Joshua-Tor L
eLife (2020) 9 [ Pubmed: 32808929 DOI: doi:10.7554/eLife.58622 ]
- Origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 1 (44 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
- Orc-o1aaa (296 kDa, Complex from Homo sapiens)
ORC-O2-5: Human Origin Recognition Complex (ORC) with subunits 2,3,4,5
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7jpq
Q-score: 0.494
Jaremko MJ, On KF, Thomas DR, Stillman B, Joshua-Tor L
eLife (2020) 9 [ Pubmed: 32808929 DOI: doi:10.7554/eLife.58622 ]
- Origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Orc-o2-5 (252 kDa, Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
Cryo-EM map of semi-attached mutant OCCM-DNA complex (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6wgc
Q-score: 0.301
Yuan Z, Schneider S, Dodd T, Riera A, Bai L, Yan C, Magdalou I, Ivanov I, Stillman B, Li H, Speck C
PNAS (2020) 117 pp. 17747-17756 [ Pubmed: 32669428 DOI: doi:10.1073/pnas.2006231117 ]
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 6 (58 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
- Dna (41-mer) (12 kDa, DNA from Saccharomyces cerevisiae)
- Orc-cdc6-mcm3-mcm7-dsdna (Complex from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Dna (41-mer) (12 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM map of mutant Mcm2-7 hexamer with Mcm6 WHD truncation
Resolution: 7.7 Å
EM Method: Single-particle
Fitted PDBs: 6wgf
Q-score: 0.064
Yuan Z, Schneider S, Dodd T, Riera A, Bai L, Yan C, Magdalou I, Ivanov I, Stillman B, Li H, Speck C
PNAS (2020) 117 pp. 17747-17756 [ Pubmed: 32669428 DOI: doi:10.1073/pnas.2006231117 ]
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Mcm2-7 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM map of pre-insertion mutant OCCM-DNA complex (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation)
Resolution: 8.1 Å
EM Method: Single-particle
Fitted PDBs: 6wgg
Q-score: 0.104
Yuan Z, Schneider S, Dodd T, Riera A, Bai L, Yan C, Magdalou I, Ivanov I, Stillman B, Li H, Speck C
PNAS (2020) 117 pp. 17747-17756 [ Pubmed: 32669428 DOI: doi:10.1073/pnas.2006231117 ]
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Orc-cdc6-mcm2-7-dsdna (Complex from Saccharomyces cerevisiae)
- Cell division control protein 6 (58 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
- Dna (41-mer) (12 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Dna (41-mer) (12 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Cell division cycle protein cdt1 (68 kDa, Protein from Saccharomyces cerevisiae)