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Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8asi
Q-score: 0.564
Swainsbury DJK, Hawkings FR, Martin EC, Musial S, Salisbury JH, Jackson PJ, Farmer DA, Johnson MP, Siebert CA, Hitchcock A, Hunter CN
PNAS (2023) 120 pp. e2217922120-e2217922120 [ DOI: doi:10.1073/pnas.2217922120 Pubmed: 36913593 ]
- Heme c (618 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Ubiquinol-cytochrome c reductase iron-sulfur subunit (19 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Cytochrome b-c1 subunit iv (14 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Ubiquinone-10 (863 Da, Ligand)
- Cytochrome c1 (30 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Cytochrome b (50 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Four subunit cytochrome b-c1 complex from rhodobacter sphaeroides (Complex from Cereibacter sphaeroides)
Resolution: 3.75 Å
EM Method: Single-particle
Fitted PDBs: 8asj
Q-score: 0.504
Swainsbury DJK, Hawkings FR, Martin EC, Musial S, Salisbury JH, Jackson PJ, Farmer DA, Johnson MP, Siebert CA, Hitchcock A, Hunter CN
PNAS (2023) 120 pp. e2217922120-e2217922120 [ DOI: doi:10.1073/pnas.2217922120 Pubmed: 36913593 ]
- Heme c (618 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Ubiquinol-cytochrome c reductase iron-sulfur subunit (19 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Cytochrome b-c1 subunit iv (14 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Ubiquinone-10 (863 Da, Ligand)
- Cytochrome c1 (30 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Cytochrome b (50 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Four subunit cytochrome b-c1 complex from rhodobacter sphaeroides (Complex from Cereibacter sphaeroides)
Cryo pFIB/SEM of PEG beads (test sample)
Dumoux M, Glen T, Smith JLR, Ho EML, Perdigão LMA, Pennington A, Klumpe S, Yee NBY, Farmer DA, Lai PYA, Bowles W, Kelley R, Plitzko JM, Wu L, Basham M, Clare DK, Siebert CA, Darrow MC, Naismith JH, Grange M
Elife (2023) 12 [ DOI: 10.7554/elife.83623 Pubmed: 36805107 ]
Image sets:
Cryo serial FIB SEM of mouse brain tissue
Dumoux M, Glen T, Smith JLR, Ho EML, Perdigão LMA, Pennington A, Klumpe S, Yee NBY, Farmer DA, Lai PYA, Bowles W, Kelley R, Plitzko JM, Wu L, Basham M, Clare DK, Siebert CA, Darrow MC, Naismith JH, Grange M
Elife (2023) 12 [ DOI: 10.7554/elife.83623 Pubmed: 36805107 ]
Image sets:
Cryo serial FIB/SEM of HeLa cells
Dumoux M, Glen T, Smith JLR, Ho EML, Perdigão LMA, Pennington A, Klumpe S, Yee NBY, Farmer DA, Lai PYA, Bowles W, Kelley R, Plitzko JM, Wu L, Basham M, Clare DK, Siebert CA, Darrow MC, Naismith JH, Grange M
Elife (2023) 12 [ DOI: 10.7554/elife.83623 Pubmed: 36805107 ]
Image sets:
Cryo serial FIB/SEM of mouse heart tissue
Dumoux M, Glen T, Smith JLR, Ho EML, Perdigão LMA, Pennington A, Klumpe S, Yee NBY, Farmer DA, Lai PYA, Bowles W, Kelley R, Plitzko JM, Wu L, Basham M, Clare DK, Siebert CA, Darrow MC, Naismith JH, Grange M
Elife (2023) 12 [ DOI: 10.7554/elife.83623 Pubmed: 36805107 ]
Image sets:
Cryo serial FIB/SEM of RPE-1 cells
Dumoux M, Glen T, Smith JLR, Ho EML, Perdigão LMA, Pennington A, Klumpe S, Yee NBY, Farmer DA, Lai PYA, Bowles W, Kelley R, Plitzko JM, Wu L, Basham M, Clare DK, Siebert CA, Darrow MC, Naismith JH, Grange M
Elife (2023) 12 [ DOI: 10.7554/elife.83623 Pubmed: 36805107 ]
Image sets:
Cryo serial FIB/SEM of Saccharomyces cerevisiae
Dumoux M, Glen T, Smith JLR, Ho EML, Perdigão LMA, Pennington A, Klumpe S, Yee NBY, Farmer DA, Lai PYA, Bowles W, Kelley R, Plitzko JM, Wu L, Basham M, Clare DK, Siebert CA, Darrow MC, Naismith JH, Grange M
Elife (2023) 12 [ DOI: 10.7554/elife.83623 Pubmed: 36805107 ]
Image sets:
Cryo serial FIB/SEM of Vero cells
Dumoux M, Glen T, Smith JLR, Ho EML, Perdigão LMA, Pennington A, Klumpe S, Yee NBY, Farmer DA, Lai PYA, Bowles W, Kelley R, Plitzko JM, Wu L, Basham M, Clare DK, Siebert CA, Darrow MC, Naismith JH, Grange M
Elife (2023) 12 [ DOI: 10.7554/elife.83623 Pubmed: 36805107 ]
Image sets: