Electron Microscopy Data Bank

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primary_citation_author:"Sachs MS"

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EMD-13741

Download Map 3D View

Structure of Candida albicans 80S ribosome in complex with cycloheximide

Release date: May 25, 2022
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 7q08
Q-score: 0.517

Zgadzay Y, Kolosova O, Stetsenko A, Wu C, Bruchlen D, Usachev K, Validov S, Jenner L, Rogachev A, Yusupova G, Sachs MS, Guskov A, Yusupov M

Sci Adv (2022) 8 pp. eabn1062-eabn1062 [ DOI: doi:10.1126/sciadv.abn1062 Pubmed: 35613268 ]

Sample:

Ribosomal 60s subunit protein l3 (44 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l36 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s20 (13 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s15 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l10 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l34b (13 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l37 (10 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s13 (16 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein el41 (3 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s23b (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s8 (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s1 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l42a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l7a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s21 (9 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l31b (12 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l15 (24 kDa, Protein from Candida albicans SC5314)
5s ribosomal rna (38 kDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s18b (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s17b (15 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s4 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s2 (26 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s26 (13 kDa, Protein from Candida albicans SC5314)
Spermidine (145 Da, Ligand)
Ribosomal 60s subunit protein l5 (34 kDa, Protein from Candida albicans SC5314)
Ubiquitin-ribosomal 40s subunit protein s31 fusion protein (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s6 (27 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l22b (14 kDa, Protein from Candida albicans SC5314)
5.8s ribosomal rna (50 kDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s19a (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s30 (7 kDa, Protein from Candida albicans SC5314)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s16a (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l28 (16 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l17b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l33a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s11a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s3 (27 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l25 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s14b (14 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s22-a (14 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l39 (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l35a (14 kDa, Protein from Candida albicans SC5314)
18s ribosomal rna (575 kDa, RNA from Candida albicans SC5314)
Ribosomal protein l19 (21 kDa, Protein from Candida albicans SC5314)
Habp4_pai-rbp1 domain-containing protein (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l26b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l4b (39 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l27 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l16a (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s24 (15 kDa, Protein from Candida albicans SC5314)
25s ribosomal rna (1 MDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s28b (7 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l29 (7 kDa, Protein from Candida albicans SC5314)
Zinc ion (65 Da, Ligand)
Ribosomal 60s subunit protein l24a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l14b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l43a (10 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l6 (19 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l18a (20 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l2a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s12 (15 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l8 (28 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s9b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s29a (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l11b (19 kDa, Protein from Candida albicans SC5314)
4-{(2r)-2-[(1s,3s,5s)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione (281 Da, Ligand)
40s ribosomal protein s25 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l21a (18 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s5 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s10a (13 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s0 (28 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l38 (8 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l23b (14 kDa, Protein from Candida albicans SC5314)
Candida albicans 80s ribosome (3 MDa, Complex from Candida albicans SC5314)
Ribosomal 60s subunit protein l32 (14 kDa, Protein from Candida albicans SC5314)
Rpl40bp (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l9b (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l20 (20 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s27 (8 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s7 (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l13 (23 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l30 (11 kDa, Protein from Candida albicans SC5314)

[Show more]

EMD-13750

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Cryo-EM map of the Candida albicans 80S ribosome in complex with blasticidin s

Release date: May 25, 2022
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 7q0r
Q-score: 0.448

Zgadzay Y, Kolosova O, Stetsenko A, Wu C, Bruchlen D, Usachev K, Validov S, Jenner L, Rogachev A, Yusupova G, Sachs MS, Guskov A, Yusupov M

Sci Adv (2022) 8 pp. eabn1062-eabn1062 [ DOI: doi:10.1126/sciadv.abn1062 Pubmed: 35613268 ]

Sample:

60s ribosomal protein l36 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s20 (13 kDa, Protein from Candida albicans SC5314)
Blasticidin s (422 Da, Ligand)
Ribosomal 40s subunit protein s15 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l10 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l34b (13 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l37 (10 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s13 (16 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l41 (3 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s23b (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s8 (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s1 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l42a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l7a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s21 (9 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l31b (12 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l15 (24 kDa, Protein from Candida albicans SC5314)
5s ribosomal rna (38 kDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s18b (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s17b (15 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s4 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s2 (26 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s26 (13 kDa, Protein from Candida albicans SC5314)
Spermidine (145 Da, Ligand)
Ribosomal 60s subunit protein l5 (34 kDa, Protein from Candida albicans SC5314)
Ubiquitin-ribosomal 40s subunit protein s31 fusion protein (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s6 (27 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l22b (14 kDa, Protein from Candida albicans SC5314)
5.8s ribosomal rna (50 kDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s19a (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s30 (7 kDa, Protein from Candida albicans SC5314)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s16a (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l28 (16 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l17b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l33a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s11a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s3 (27 kDa, Protein from Candida albicans SC5314)
Mixture of the endogenous e-trna (24 kDa, RNA from Candida albicans SC5314)
Ribosomal 60s subunit protein l25 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s14b (14 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s22-a (14 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l39 (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l35a (14 kDa, Protein from Candida albicans SC5314)
18s ribosomal rna (575 kDa, RNA from Candida albicans SC5314)
Ribosomal protein l19 (21 kDa, Protein from Candida albicans SC5314)
Habp4_pai-rbp1 domain-containing protein (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l26b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l4b (39 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l27 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l16a (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s24 (15 kDa, Protein from Candida albicans SC5314)
25s ribosomal rna (1 MDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s28b (7 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l3 (44 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l29 (7 kDa, Protein from Candida albicans SC5314)
Zinc ion (65 Da, Ligand)
Ribosomal 60s subunit protein l24a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l14b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l43a (10 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l6 (19 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l18a (20 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l2a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s12 (15 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l8 (28 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s9b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s29a (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l11b (19 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s25 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l21a (18 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s5 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s10a (13 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s0 (28 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l38 (8 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l23b (14 kDa, Protein from Candida albicans SC5314)
Candida albicans 80s ribosome (3 MDa, Complex from Candida albicans SC5314)
Ribosomal 60s subunit protein l32 (14 kDa, Protein from Candida albicans SC5314)
Rpl40bp (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l9b (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l20 (20 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s27 (8 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s7 (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l13 (23 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l30 (11 kDa, Protein from Candida albicans SC5314)

[Show more]

EMD-13737

Download Map 3D View

Cryo-EM map of the vacant Candida albicans 80S ribosome

Release date: May 18, 2022
Resolution: 2.32 Å
EM Method: Single-particle
Fitted PDBs: 7pzy
Q-score: 0.53

Zgadzay Y, Kolosova O, Stetsenko A, Wu C, Bruchlen D, Usachev K, Validov S, Jenner L, Rogachev A, Yusupova G, Sachs MS, Guskov A, Yusupov M

Sci Adv (2022) 8 pp. eabn1062-eabn1062 [ DOI: doi:10.1126/sciadv.abn1062 Pubmed: 35613268 ]

Sample:

60s ribosomal protein l36 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s20 (13 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s15 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l10 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l34b (13 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l37 (10 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s13 (16 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l41 (3 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s23b (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s8 (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s1 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l42a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l7a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s21 (9 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l31b (12 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l15 (24 kDa, Protein from Candida albicans SC5314)
5s ribosomal rna (38 kDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s18b (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s17b (15 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s4 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s2 (26 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s26 (13 kDa, Protein from Candida albicans SC5314)
Spermidine (145 Da, Ligand)
Water (18 Da, Ligand)
Ribosomal 60s subunit protein l5 (34 kDa, Protein from Candida albicans SC5314)
Ubiquitin-ribosomal 40s subunit protein s31 fusion protein (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s6 (27 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l22b (14 kDa, Protein from Candida albicans SC5314)
5.8s ribosomal rna (50 kDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s19a (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s30 (7 kDa, Protein from Candida albicans SC5314)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s16a (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l28 (16 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l17b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l33a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s11a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s3 (27 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l25 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s14b (14 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s22-a (14 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l39 (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l35a (14 kDa, Protein from Candida albicans SC5314)
18s ribosomal rna (575 kDa, RNA from Candida albicans SC5314)
Ribosomal protein l19 (21 kDa, Protein from Candida albicans SC5314)
Habp4_pai-rbp1 domain-containing protein (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l26b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l4b (39 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l27 (15 kDa, Protein from Candida albicans SC5314)
Mixture of endogenous e-trnas (24 kDa, RNA from Candida albicans SC5314)
Ribosomal 60s subunit protein l16a (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s24 (15 kDa, Protein from Candida albicans SC5314)
25s ribosomal rna (1 MDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s28b (7 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l3 (44 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l29 (7 kDa, Protein from Candida albicans SC5314)
Zinc ion (65 Da, Ligand)
Ribosomal 60s subunit protein l24a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l14b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l43a (10 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l6 (19 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l18a (20 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l2a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s12 (15 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l8 (28 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s9b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s29a (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l11b (19 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s25 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l21a (18 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s5 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s10a (13 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s0 (28 kDa, Protein from Candida albicans SC5314)
1,4-diaminobutane (88 Da, Ligand)
Ribosomal 60s subunit protein l38 (8 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l23b (14 kDa, Protein from Candida albicans SC5314)
Candida albicans 80s ribosome (3 MDa, Complex from Candida albicans SC5314)
Ribosomal 60s subunit protein l32 (14 kDa, Protein from Candida albicans SC5314)
Rpl40bp (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l9b (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l20 (20 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s27 (8 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s7 (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l13 (23 kDa, Protein from Candida albicans SC5314)
Magnesium ion (24 Da, Ligand)
Ribosomal 60s subunit protein l30 (11 kDa, Protein from Candida albicans SC5314)

[Show more]

EMD-13744

Download Map 3D View

Cryo-EM map of Candida albicans 80S ribosome in complex with phyllanthoside

Release date: May 18, 2022
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 7q0f
Q-score: 0.521

Zgadzay Y, Kolosova O, Stetsenko A, Wu C, Bruchlen D, Usachev K, Validov S, Jenner L, Rogachev A, Yusupova G, Sachs MS, Guskov A, Yusupov M

Sci Adv (2022) 8 pp. eabn1062-eabn1062 [ DOI: doi:10.1126/sciadv.abn1062 Pubmed: 35613268 ]

Sample:

Ribosomal 60s subunit protein l3 (44 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l36 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s20 (13 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s15 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l10 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l34b (13 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l37 (10 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s13 (16 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein el41 (3 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s23b (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s8 (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s1 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l42a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l7a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s21 (9 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l31b (12 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l15 (24 kDa, Protein from Candida albicans SC5314)
5s ribosomal rna (38 kDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s18b (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s17b (15 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s4 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s2 (26 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s26 (13 kDa, Protein from Candida albicans SC5314)
Spermidine (145 Da, Ligand)
Ribosomal 60s subunit protein l5 (34 kDa, Protein from Candida albicans SC5314)
Ubiquitin-ribosomal 40s subunit protein s31 fusion protein (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s6 (27 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l22b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s19a (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s30 (7 kDa, Protein from Candida albicans SC5314)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s16a (15 kDa, Protein from Candida albicans SC5314)
3-o-acetyl-2-o-(3-o-acetyl-6-deoxy-beta-d-glucopyranosyl)-6-deoxy-1-o-{[(2r,2's,3a'r,4''s,5''r,6's,7a's)-5''-methyl-4''-{[(2e)-3-phenylprop-2-enoyl]oxy}decahydrodispiro[oxirane-2,3'-[1]benzofuran-2',2''-pyran]-6'-yl]carbonyl}-beta-d-glucopyranose (804 Da, Ligand)
Ribosomal 60s subunit protein l28 (16 kDa, Protein from Candida albicans SC5314)
5.8s ribosomal rna (50 kDa, RNA from Candida albicans)
Ribosomal 60s subunit protein l17b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l33a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s11a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s3 (27 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l25 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s14b (14 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s22-a (14 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l39 (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l35a (14 kDa, Protein from Candida albicans SC5314)
18s ribosomal rna (575 kDa, RNA from Candida albicans SC5314)
Ribosomal protein l19 (21 kDa, Protein from Candida albicans SC5314)
Habp4_pai-rbp1 domain-containing protein (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l26b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l4b (39 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l27 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l16a (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s24 (15 kDa, Protein from Candida albicans SC5314)
25s ribosomal rna (1 MDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s28b (7 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l29 (7 kDa, Protein from Candida albicans SC5314)
Zinc ion (65 Da, Ligand)
Ribosomal 60s subunit protein l24a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l14b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l43a (10 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l6 (19 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l18a (20 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l2a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s12 (15 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l8 (28 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s9b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s29a (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l11b (19 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s25 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l21a (18 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s5 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s10a (13 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s0 (28 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l38 (8 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l23b (14 kDa, Protein from Candida albicans SC5314)
Candida albicans 80s ribosome (3 MDa, Complex from Candida albicans SC5314)
Ribosomal 60s subunit protein l32 (14 kDa, Protein from Candida albicans SC5314)
Rpl40bp (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l9b (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l20 (20 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s27 (8 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s7 (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l13 (23 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l30 (11 kDa, Protein from Candida albicans SC5314)

[Show more]

EMD-13749

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Cryo-EM map of the Candida albicans 80S ribosome in complex with anisomycin

Release date: May 18, 2022
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7q0p
Q-score: 0.41

Zgadzay Y, Kolosova O, Stetsenko A, Wu C, Bruchlen D, Usachev K, Validov S, Jenner L, Rogachev A, Yusupova G, Sachs MS, Guskov A, Yusupov M

Sci Adv (2022) 8 pp. eabn1062-eabn1062 [ DOI: doi:10.1126/sciadv.abn1062 Pubmed: 35613268 ]

Sample:

Ribosomal 60s subunit protein l3 (44 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l36 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s20 (13 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s15 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l10 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l34b (13 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l37 (10 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s13 (16 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l41 (3 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s23b (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s8 (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s1 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l42a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l7a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s21 (9 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l31b (12 kDa, Protein from Candida albicans SC5314)
Ribosomal protein l15 (24 kDa, Protein from Candida albicans SC5314)
5s ribosomal rna (38 kDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s18b (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s17b (15 kDa, Protein from Candida albicans SC5314)
Mixture of endogenous e-trna (24 kDa, RNA from Candida albicans SC5314)
40s ribosomal protein s4 (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s2 (26 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s26 (13 kDa, Protein from Candida albicans SC5314)
Anisomycin (265 Da, Ligand)
Spermidine (145 Da, Ligand)
Ribosomal 60s subunit protein l5 (34 kDa, Protein from Candida albicans SC5314)
Ubiquitin-ribosomal 40s subunit protein s31 fusion protein (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s6 (27 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l22b (14 kDa, Protein from Candida albicans SC5314)
5.8s ribosomal rna (50 kDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s19a (16 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s30 (7 kDa, Protein from Candida albicans SC5314)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s16a (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l28 (16 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l17b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l33a (12 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s11a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s3 (27 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l25 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s14b (14 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l39 (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l35a (14 kDa, Protein from Candida albicans SC5314)
18s ribosomal rna (575 kDa, RNA from Candida albicans SC5314)
Ribosomal protein l19 (21 kDa, Protein from Candida albicans SC5314)
Habp4_pai-rbp1 domain-containing protein (29 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l26b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l4b (39 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l27 (15 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l16a (22 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s24 (15 kDa, Protein from Candida albicans SC5314)
25s ribosomal rna (1 MDa, RNA from Candida albicans SC5314)
Ribosomal 40s subunit protein s28b (7 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l29 (7 kDa, Protein from Candida albicans SC5314)
Zinc ion (65 Da, Ligand)
Ribosomal 60s subunit protein l24a (17 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l14b (14 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l43a (10 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l6 (19 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l18a (20 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l2a (27 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s12 (15 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l8 (28 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s9b (21 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s29a (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l11b (19 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s25 (11 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l21a (18 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s5 (25 kDa, Protein from Candida albicans SC5314)
Ribosomal 40s subunit protein s10a (13 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s0 (28 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l38 (8 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l23b (14 kDa, Protein from Candida albicans SC5314)
Candida albicans 80s ribosome (3 MDa, Complex from Candida albicans SC5314)
Ribosomal 60s subunit protein l32 (14 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s22-b (14 kDa, Protein from Candida albicans SC5314)
Rpl40bp (6 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l9b (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l20 (20 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s27 (8 kDa, Protein from Candida albicans SC5314)
40s ribosomal protein s7 (21 kDa, Protein from Candida albicans SC5314)
60s ribosomal protein l13 (23 kDa, Protein from Candida albicans SC5314)
Ribosomal 60s subunit protein l30 (11 kDa, Protein from Candida albicans SC5314)

[Show more]

EMPIAR-10695

Release date: Jan. 24, 2022
Dataset size: 6.9 TB
Experiment type: EMDB

van der Stel AX, Gordon ER, Sengupta A, Martínez AK, Klepacki D, Perry TN, Herrero Del Valle A, Vázquez-Laslop N, Sachs MS, Cruz-Vera LR, Innis CA

Nat Commun (2021) 12 [ DOI: 10.1038/s41467-021-25663-8 Pubmed: 34504068 ]

Image sets:

EMD-24307

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Structure of the translating Neurospora crassa ribosome arrested by cycloheximide

Release date: Dec. 1, 2021
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7r81
Q-score: 0.539

Shen L, Su Z, Yang K, Wu C, Becker T, Bell-Pedersen D, Zhang J, Sachs MS

PNAS (2021) 118 [ Pubmed: 34815343 DOI: doi:10.1073/pnas.2111862118 ]

Sample:

40s ribosomal protein s18 (17 kDa, Protein from Neurospora crassa)
40s ribosomal protein s11 (18 kDa, Protein from Neurospora crassa)
Probable 40s ribosomal protein s10-b (18 kDa, Protein from Neurospora crassa)
60s ribosomal protein l33 (12 kDa, Protein from Neurospora crassa)
Spermidine (145 Da, Ligand)
60s ribosomal protein l38 (9 kDa, Protein from Neurospora crassa)
Hypothetical protein ncu16635 (el41) (3 kDa, Protein from Neurospora crassa)
60s ribosomal protein l18 (20 kDa, Protein from Neurospora crassa)
40s ribosomal protein s28 (7 kDa, Protein from Neurospora crassa)
40s ribosomal protein s27 (8 kDa, Protein from Neurospora crassa)
60s ribosomal protein l23 (14 kDa, Protein from Neurospora crassa)
60s ribosomal protein l4-a (38 kDa, Protein from Neurospora crassa)
40s ribosomal protein s17 (16 kDa, Protein from Neurospora crassa)
Nascent peptide (Complex from Neurospora crassa)
40s ribosomal protein s19 (16 kDa, Protein from Neurospora crassa)
60s ribosomal protein l10 (25 kDa, Protein from Neurospora crassa)
60s ribosomal protein l30 (11 kDa, Protein from Neurospora crassa)
Ribosomal protein l14 (15 kDa, Protein from Neurospora crassa)
40s ribosomal protein s12 (16 kDa, Protein from Neurospora crassa)
Ribosomal protein l26 (15 kDa, Protein from Neurospora crassa)
Nascent peptide (358 Da, Protein from Neurospora crassa)
40s ribosomal protein s9 (21 kDa, Protein from Neurospora crassa)
40s ribosomal protein s14 (us11) (16 kDa, Protein from Neurospora crassa)
60s ribosomal protein l36 (11 kDa, Protein from Neurospora crassa)
60s ribosomal protein l35 (14 kDa, Protein from Neurospora crassa)
60s ribosomal protein l17 (20 kDa, Protein from Neurospora crassa)
60s ribosomal protein l43 (10 kDa, Protein from Neurospora crassa)
60s ribosomal protein l13 (23 kDa, Protein from Neurospora crassa)
60s ribosomal protein l32 (15 kDa, Protein from Neurospora crassa)
60s ribosomal protein l28 (16 kDa, Protein from Neurospora crassa)
26s rrna (1 MDa, RNA from Neurospora crassa)
Ubiquitin-40s ribosomal protein s27a (17 kDa, Protein from Neurospora crassa)
60s ribosomal protein l29 (7 kDa, Protein from Neurospora crassa)
40s ribosomal subunit (Complex from Neurospora crassa)
40s ribosomal protein s13 (16 kDa, Protein from Neurospora crassa)
60s ribosomal protein l34 (13 kDa, Protein from Neurospora crassa)
60s ribosomal protein l40 (6 kDa, Protein from Neurospora crassa)
60s ribosomal protein l15 (24 kDa, Protein from Neurospora crassa)
5.8s rrna (50 kDa, RNA from Neurospora crassa)
Structure of the translating neurospora crassa ribosome arrested by cycloheximide (Complex)
40s ribosomal protein s20 (13 kDa, Protein from Neurospora crassa)
60s ribosomal protein (14 kDa, Protein from Neurospora crassa)
5s rrna (38 kDa, RNA from Neurospora crassa)
40s ribosomal protein s6 (27 kDa, Protein from Neurospora crassa)
60s ribosomal protein l8 (29 kDa, Protein from Neurospora crassa)
60s ribosomal protein l3 (44 kDa, Protein from Neurospora crassa)
40s ribosomal protein s8 (23 kDa, Protein from Neurospora crassa)
60s ribosomal protein l5 (34 kDa, Protein from Neurospora crassa)
60s ribosomal protein l2 (27 kDa, Protein from Neurospora crassa)
40s ribosomal protein s24 (15 kDa, Protein from Neurospora crassa)
Guanine nucleotide-binding protein subunit beta-like protein (35 kDa, Protein from Neurospora crassa)
60s ribosomal protein l7 (28 kDa, Protein from Neurospora crassa)
40s ribosomal protein s7 (22 kDa, Protein from Neurospora crassa)
60s ribosomal protein l24 (17 kDa, Protein from Neurospora crassa)
60s ribosomal protein l44 (11 kDa, Protein from Neurospora crassa)
40s ribosomal protein s2 (28 kDa, Protein from Neurospora crassa)
Ribosomal protein srp1 (18 kDa, Protein from Neurospora crassa)
40s ribosomal protein s21 (9 kDa, Protein from Neurospora crassa)
Ribosomal protein l37 (10 kDa, Protein from Neurospora crassa)
18s rrna (578 kDa, RNA from Neurospora crassa)
40s ribosomal protein s16 (15 kDa, Protein from Neurospora crassa)
40s ribosomal protein s22 (14 kDa, Protein from Neurospora crassa)
Ribosomal protein l19 (22 kDa, Protein from Neurospora crassa)
60s ribosomal subunit (Complex from Neurospora crassa)
40s ribosomal protein s0 (31 kDa, Protein from Neurospora crassa)
60s ribosomal protein l16 (26 kDa, Protein from Neurospora crassa)
40s ribosomal protein s26e (13 kDa, Protein from Neurospora crassa)
60s ribosomal protein l6 (22 kDa, Protein from Neurospora crassa)
40s ribosomal protein s25 (10 kDa, Protein from Neurospora crassa)
40s ribosomal protein s29 (6 kDa, Protein from Neurospora crassa)
40s ribosomal protein s4 (29 kDa, Protein from Neurospora crassa)
Mrna (Complex from Neurospora crassa)
Ribosomal_l28e domain-containing protein (16 kDa, Protein from Neurospora crassa)
60s ribosomal protein l39 (6 kDa, Protein from Neurospora crassa)
Mrna (5 kDa, RNA from Neurospora crassa)
60s ribosomal protein l27 (el27) (15 kDa, Protein from Neurospora crassa)
40s ribosomal protein s23 (15 kDa, Protein from Neurospora crassa)
60s ribosomal protein l25 (17 kDa, Protein from Neurospora crassa)
Trna (24 kDa, RNA from Neurospora crassa)
60s ribosomal protein l20 (20 kDa, Protein from Neurospora crassa)
Magnesium ion (24 Da, Ligand)
Ribosomal protein s12 (17 kDa, Protein from Neurospora crassa)
40s ribosomal protein s5 (23 kDa, Protein from Neurospora crassa)
60s ribosomal protein l22 (14 kDa, Protein from Neurospora crassa)
4-{(2r)-2-[(1s,3s,5s)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione (281 Da, Ligand)
60s ribosomal protein l11 (20 kDa, Protein from Neurospora crassa)
60s ribosomal protein l9 (21 kDa, Protein from Neurospora crassa)
Trna (Complex from Neurospora crassa)
Cytoplasmic ribosomal protein 10 (28 kDa, Protein from Neurospora crassa)
40s ribosomal protein s30 (6 kDa, Protein from Neurospora crassa)
40s ribosomal protein s1 (29 kDa, Protein from Neurospora crassa)

[Show more]

EMD-12693

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Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan

Release date: Sept. 1, 2021
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7o19
Q-score: 0.555

van der Stel AX, Gordon ER, Sengupta A, Martinez AK, Klepacki D, Perry TN, Herrero Del Valle A, Vazquez-Laslop N, Sachs MS, Cruz-Vera LR, Innis CA

Nat Commun (2021) 12 pp. 5340-5340 [ DOI: doi:10.1038/s41467-021-25663-8 Pubmed: 34504068 ]

Sample:

30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
30s subunit (Complex from Escherichia coli K-12)
Tryptophan (204 Da, Ligand)
Water (18 Da, Ligand)
Ribosomal rna 23s (939 kDa, RNA from Escherichia coli K-12)
50s subunit (Complex from Escherichia coli K-12)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
Ribosomal rna 5s (38 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
P-site trna-pro (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
P-site trna-pro - tnac nascent chain (Complex from Escherichia coli K-12)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
Tryptophanase leader peptide (2 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
Mrna (Complex from Escherichia coli K-12)
70s ribosome stalled during translation of tnac with pro24 in the p-site. (Complex from Escherichia coli K-12)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli K-12)
Mrna (2 kDa, RNA from Escherichia coli K-12)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
Ribosomal rna 16s (497 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)

[Show more]

EMD-12694

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Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome complex stalled in response to L-tryptophan

Release date: Sept. 1, 2021
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7o1a
Q-score: 0.573

van der Stel AX, Gordon ER, Sengupta A, Martinez AK, Klepacki D, Perry TN, Herrero Del Valle A, Vazquez-Laslop N, Sachs MS, Cruz-Vera LR, Innis CA

Nat Commun (2021) 12 pp. 5340-5340 [ DOI: doi:10.1038/s41467-021-25663-8 Pubmed: 34504068 ]

Sample:

50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
30s subunit (Complex from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
Tryptophan (204 Da, Ligand)
Mrna (2 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli (strain K12))
Water (18 Da, Ligand)
Ribosomal rna 23s (939 kDa, RNA from Escherichia coli K-12)
50s subunit (Complex from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
Ribosomal rna 5s (38 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
Potassium ion (39 Da, Ligand)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
P-site trna-pro - tnac-(r23f) nascent chain (Complex from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
Mrna (Complex from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
Tnac - tryptophanase leader peptide - r23f (2 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
P-site trna-pro (24 kDa, RNA from Escherichia coli (strain K12))
Zinc ion (65 Da, Ligand)
Ribosomal rna 16s (497 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
70s ribosome stalled during translation of tnac(r23f) with pro24 in the p-site. (Complex from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))

[Show more]

EMD-12695

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Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome-RF2 complex stalled in response to L-tryptophan

Release date: Sept. 1, 2021
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7o1c
Q-score: 0.555

van der Stel AX, Gordon ER, Sengupta A, Martinez AK, Klepacki D, Perry TN, Herrero Del Valle A, Vazquez-Laslop N, Sachs MS, Cruz-Vera LR, Innis CA

Nat Commun (2021) 12 pp. 5340-5340 [ DOI: doi:10.1038/s41467-021-25663-8 Pubmed: 34504068 ]

Sample:

50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
30s subunit (Complex from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
Tryptophan (204 Da, Ligand)
Water (18 Da, Ligand)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli (strain K12))
Ribosomal rna 23s (939 kDa, RNA from Escherichia coli K-12)
50s subunit (Complex from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
Ribosomal rna 5s (38 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
Potassium ion (39 Da, Ligand)
Mrna (3 kDa, RNA from Escherichia coli K-12)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
P-site trna-pro - tnac-(r23f) nascent chain (Complex from Escherichia coli K-12)
P-site trna-pro (24 kDa, RNA from Escherichia coli K-12)
Tnac-(r23f) - tryptophanase leader peptide (2 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli (strain K12))
Peptide chain release factor rf2 (41 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
Mrna (Complex from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
Zinc ion (65 Da, Ligand)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
Ribosomal rna 16s (497 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
70s ribosome stalled during translation of tnac(r23f) with pro24 in the p-site and release factor 2 in the a-site. (Complex from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
Peptide chain release factor rf2 (Complex from Escherichia coli K-12)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))

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EMDB [9]
EMPIAR [1]

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