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Architecture of the human erythrocyte ankyrin-1 complex
Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Calì T, Clarke OB
Nat Struct Mol Biol (2022) 29 pp. 706-718 [ Pubmed: 35835865 DOI: 10.1038/s41594-022-00792-w ]
Image sets:
Band 3-Glycophorin A complex, outward facing
Resolution: 2.35 Å
EM Method: Single-particle
Fitted PDBs: 7uz3
Q-score: 0.701
Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Cali T, Clarke OB
Nat Struct Mol Biol (2022) 29 pp. 706-718 [ Pubmed: 35835865 DOI: doi:10.1038/s41594-022-00792-w ]
- Glycophorin-a (16 kDa, Protein from human)
- Band 3 anion transport protein (101 kDa, Protein from human)
- Cholesterol (386 Da, Ligand)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Water (18 Da, Ligand)
- Band 3 anion exchanger complexed with glycophorin a, in outward facing state. (Complex from Homo sapiens)
Erythrocyte ankyrin-1 complex class 2 local refinement of AQP1 (C4 symmetry applied)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7uze
Q-score: 0.701
Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Cali T, Clarke OB
Nat Struct Mol Biol (2022) 29 pp. 706-718 [ Pubmed: 35835865 DOI: doi:10.1038/s41594-022-00792-w ]
- Aquaporin-1 (28 kDa, Protein from human)
- Local refinement of aquaporin 1 in class 2 of ankyrin complex; local c4 symmetry applied. (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Water (18 Da, Ligand)
Local refinement of RhAG-RhCE-ANK1(AR1-5), from consensus refinement of all classes
Resolution: 2.17 Å
EM Method: Single-particle
Fitted PDBs: 7uzq
Q-score: 0.749
Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Cali T, Clarke OB
Nat Struct Mol Biol (2022) 29 pp. 706-718 [ Pubmed: 35835865 DOI: doi:10.1038/s41594-022-00792-w ]
- Cholesterol (386 Da, Ligand)
- Blood group rh(ce) polypeptide (45 kDa, Protein from Homo sapiens)
- Ammonium transporter rh type a (44 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Digitonin (1 kDa, Ligand)
- Ankyrin complex mixture purified from digitonin-solubilized erythrocyte ghost membranes (Complex from Homo sapiens)
- Ankyrin-1 (21 kDa, Protein from Homo sapiens)
Protein 4.2 (local refinement from consensus reconstruction of ankyrin complex classes)
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 7uzs
Q-score: 0.735
Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Cali T, Clarke OB
Nat Struct Mol Biol (2022) 29 pp. 706-718 [ Pubmed: 35835865 DOI: doi:10.1038/s41594-022-00792-w ]
- Ankyrin complex mixture purified from digitonin-solubilized erythrocyte ghost membranes (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Protein 4.2 (77 kDa, Protein from Homo sapiens)
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7uzu
Q-score: 0.713
Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Cali T, Clarke OB
Nat Struct Mol Biol (2022) 29 pp. 706-718 [ Pubmed: 35835865 DOI: doi:10.1038/s41594-022-00792-w ]
- Ankyrin complex mixture purified from digitonin-solubilized erythrocyte ghost membranes (Complex from Homo sapiens)
- Band 3 anion transport protein (101 kDa, Protein from Homo sapiens)
- Ankyrin-1 (206 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7uzv
Q-score: 0.649
Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Cali T, Clarke OB
Nat Struct Mol Biol (2022) 29 pp. 706-718 [ Pubmed: 35835865 DOI: doi:10.1038/s41594-022-00792-w ]
Band 3-I-TM local refinement from erythrocyte ankyrin-1 complex consensus reconstruction
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7v07
Q-score: 0.604
Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Cali T, Clarke OB
Nat Struct Mol Biol (2022) 29 pp. 706-718 [ Pubmed: 35835865 DOI: doi:10.1038/s41594-022-00792-w ]
- Glycophorin-a (16 kDa, Protein from human)
- Band 3 anion transport protein (101 kDa, Protein from human)
- Cholesterol (386 Da, Ligand)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Ankyrin complex mixture purified from digitonin-solubilized erythrocyte ghost membranes (Complex from Homo sapiens)
Consensus refinement of human erythrocyte ankyrin-1 complex (Composite map)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7v0k
Q-score: 0.703
Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Cali T, Clarke OB
Nat Struct Mol Biol (2022) 29 pp. 706-718 [ Pubmed: 35835865 DOI: doi:10.1038/s41594-022-00792-w ]
- Glycophorin-a (16 kDa, Protein from human)
- Band 3 anion transport protein (101 kDa, Protein from human)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Ankyrin-1 (206 kDa, Protein from human)
- Protein 4.2 (77 kDa, Protein from human)
- Band 3 anion exchanger complexed with glycophorin a, in outward facing state (Complex from Homo sapiens)
- Ammonium transporter rh type a (44 kDa, Protein from human)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Digitonin (1 kDa, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Water (18 Da, Ligand)
- Blood group rh(ce) polypeptide (45 kDa, Protein from human)