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Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7nf6
Q-score: 0.558
Lee Y, Wiriyasermkul P, Kongpracha P, Moriyama S, Mills DJ, Kuhlbrandt W, Nagamori S
Nat Commun (2022) 13 pp. 2708-2708 [ DOI: doi:10.1038/s41467-022-30293-9 Pubmed: 35577790 ]
- Calcium ion (40 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- B(0,+)-type amino acid transporter 1 (56 kDa, Protein from Ovis aries)
- Neutral and basic amino acid transport protein rbat (78 kDa, Protein from Ovis aries)
- B0,+at-rbat heterodimer (135 MDa, Complex from Ovis aries)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Ovine rBAT ectodomain homodimer, C2-symmetry
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7nf7
Q-score: 0.642
Lee Y, Wiriyasermkul P, Kongpracha P, Moriyama S, Mills DJ, Kuhlbrandt W, Nagamori S
Nat Commun (2022) 13 pp. 2708-2708 [ DOI: doi:10.1038/s41467-022-30293-9 Pubmed: 35577790 ]
- Ovine rbat ectodomain homodimer (79 MDa, Complex from Ovis aries)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neutral and basic amino acid transport protein rbat (78 kDa, Protein from Ovis aries)
- Calcium ion (40 Da, Ligand)
Ovine (b0,+AT-rBAT)2 hetero-tetramer, consensus refinement, C2-symmetry
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7nf8
Q-score: 0.496
Lee Y, Wiriyasermkul P, Kongpracha P, Moriyama S, Mills DJ, Kuhlbrandt W, Nagamori S
Nat Commun (2022) 13 pp. 2708-2708 [ DOI: doi:10.1038/s41467-022-30293-9 Pubmed: 35577790 ]
- Calcium ion (40 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- (b0,+at-rbat) hetero-tetramer (270 MDa, Complex from Ovis aries)
- B(0,+)-type amino acid transporter 1 (56 kDa, Protein from Ovis aries)
- Neutral and basic amino acid transport protein rbat (78 kDa, Protein from Ovis aries)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the fungal plasma membrane proton pump Pma1 in its auto-inhibited state - monomer unit
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7nxf
Q-score: 0.439
Heit S, Geurts MMG, Murphy BJ, Corey RA, Mills DJ, Kuhlbrandt W, Bublitz M
Sci Adv (2021) 7 pp. eabj5255-eabj5255 [ Pubmed: 34757782 DOI: doi:10.1126/sciadv.abj5255 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Monomeric subunit of the hexameric fungal plasma membrane proton pump in its auto-inhibited state (Cellular component from Neurospora crassa)
- Plasma membrane atpase (99 kDa, Protein from Neurospora crassa)
- Magnesium ion (24 Da, Ligand)
- Potassium ion (39 Da, Ligand)
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 7ny1
Q-score: 0.357
Heit S, Geurts MMG, Murphy BJ, Corey RA, Mills DJ, Kuhlbrandt W, Bublitz M
Sci Adv (2021) 7 pp. eabj5255-eabj5255 [ Pubmed: 34757782 DOI: doi:10.1126/sciadv.abj5255 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Plasma membrane atpase (99 kDa, Protein from Neurospora crassa)
- Magnesium ion (24 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Hexameric assembly of the fungal plasma membrane proton pump in its auto-inhibited state (Complex from Neurospora crassa)
Cryo-EM structure of respiratory complex I under turnover
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7o6y
Q-score: 0.468
Parey K, Lasham J, Mills DJ, Djurabekova A, Haapanen O, Yoga EG, Xie H, Kuhlbrandt W, Sharma V, Vonck J, Zickermann V
Sci Adv (2021) 7 pp. eabj3221-eabj3221 [ Pubmed: 34767441 DOI: doi:10.1126/sciadv.abj3221 ]
- Subunit nuam of nadh:ubiquinone oxidoreductase (complex i) (79 kDa, Protein from Candida lipolytica)
- Subunit nugm of nadh:ubiquinone oxidoreductase (complex i) (32 kDa, Protein from Candida lipolytica)
- Subunit nuzm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Subunit nipm of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Candida lipolytica)
- Subunit numm of protein nadh:ubiquinone oxidoreductase (complex i) (15 kDa, Protein from Candida lipolytica)
- Zinc ion (65 Da, Ligand)
- Subunit nebm of nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Mitochondrial nadh:ubiquinone oxidoreductase (1 MDa, Complex from Yarrowia lipolytica)
- Subunit nb8m of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Candida lipolytica)
- Nadh-ubiquinone oxidoreductase chain 3 (14 kDa, Protein from Yarrowia lipolytica)
- Subunit nujm of nadh:ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Yarrowia lipolytica)
- Subunit nb4m of protein nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Subunit niam of nadh:ubiquinone oxidoreductase (complex i) (17 kDa, Protein from Yarrowia lipolytica)
- 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (758 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Ubiquinone-9 (795 Da, Ligand)
- Subunit ni2m of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Candida lipolytica)
- Phosphatidylinositol (887 Da, Ligand)
- Subunit nuym of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Candida lipolytica)
- Subunit nu4m of nadh:ubiquinone oxidoreductase (complex i) (54 kDa, Protein from Yarrowia lipolytica)
- Subunit nuvm of nadh:ubiquinone oxidoreductase (complex i) (7 kDa, Protein from Yarrowia lipolytica)
- Nucm protein (52 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 1 (38 kDa, Protein from Yarrowia lipolytica)
- Acyl carrier protein acpm2 of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Flavin mononucleotide (456 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Subunit nimm of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Candida lipolytica)
- Subunit nb5m of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Candida lipolytica)
- Subunit nesm of nadh:ubiquinone oxidoreductase (complex i) (28 kDa, Protein from Yarrowia lipolytica)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (53 kDa, Protein from Candida lipolytica)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Subunit nuxm of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Candida lipolytica)
- Acyl carrier protein acpm1 of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Candida lipolytica)
- Nadh dehydrogenase subunit 2 (53 kDa, Protein from Yarrowia lipolytica)
- Subunit nukm of nadh:ubiquinone oxidoreductase (complex i) (23 kDa, Protein from Candida lipolytica)
- Subunit nuem of nadh:ubiquinone oxidoreductase (complex i) (42 kDa, Protein from Yarrowia lipolytica)
- Subunit nuhm of nadh:ubiquinone oxidoreductase (complex i) (27 kDa, Protein from Candida lipolytica)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Subunit ni9m of protein nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Subunit nuim of nadh:ubiquinone oxidoreductase (complex i) (25 kDa, Protein from Candida lipolytica)
- Subunit nupm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Subunit nuum of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Subunit nu5m of nadh:ubiquinone oxidoreductase (complex i) (73 kDa, Protein from Yarrowia lipolytica)
- Subunit nunm of nadh:ubiquinone oxidoreductase (complex i) (15 kDa, Protein from Candida lipolytica)
- Nadh-ubiquinone oxidoreductase chain 6 (20 kDa, Protein from Candida lipolytica)
- Subunit nufm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Subunit nb2m of nadh:ubiquinone oxidoreductase (complex i) (6 kDa, Protein from Yarrowia lipolytica)
- Subunit nb6m of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Subunit n7bm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit nidm of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Candida lipolytica)
- Subunit ni8m of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Candida lipolytica)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Yarrowia lipolytica)
Cryo-EM structure of a respiratory complex I
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7o71
Q-score: 0.644
Parey K, Lasham J, Mills DJ, Djurabekova A, Haapanen O, Yoga EG, Xie H, Kuhlbrandt W, Sharma V, Vonck J, Zickermann V
Sci Adv (2021) 7 pp. eabj3221-eabj3221 [ Pubmed: 34767441 DOI: doi:10.1126/sciadv.abj3221 ]
- Subunit nugm of nadh:ubiquinone oxidoreductase (complex i) (32 kDa, Protein from Candida lipolytica)
- Subunit nuzm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Subunit nipm of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Candida lipolytica)
- Subunit numm of protein nadh:ubiquinone oxidoreductase (complex i) (15 kDa, Protein from Candida lipolytica)
- Nadh-ubiquinone oxidoreductase chain 4 (54 kDa, Protein from Yarrowia lipolytica)
- Subunit nunm of nadh:ubiquinone oxidoreductase (complex i) (13 kDa, Protein from Candida lipolytica)
- Zinc ion (65 Da, Ligand)
- Subunit nebm of nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Mitochondrial nadh:ubiquinone oxidoreductase (1 MDa, Complex from Yarrowia lipolytica)
- Subunit nb8m of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Candida lipolytica)
- Nadh-ubiquinone oxidoreductase chain 3 (14 kDa, Protein from Yarrowia lipolytica)
- Subunit nujm of nadh:ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Yarrowia lipolytica)
- Subunit nb4m of protein nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Subunit niam of nadh:ubiquinone oxidoreductase (complex i) (17 kDa, Protein from Yarrowia lipolytica)
- 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (758 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Subunit ni2m of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Candida lipolytica)
- Phosphatidylinositol (887 Da, Ligand)
- Subunit nuym of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Candida lipolytica)
- Subunit nuvm of nadh:ubiquinone oxidoreductase (complex i) (7 kDa, Protein from Yarrowia lipolytica)
- Nucm protein (52 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 1 (38 kDa, Protein from Yarrowia lipolytica)
- Acyl carrier protein acpm2 of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Flavin mononucleotide (456 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Water (18 Da, Ligand)
- Subunit nimm of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Candida lipolytica)
- Subunit nb5m of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Candida lipolytica)
- Subunit nesm of nadh:ubiquinone oxidoreductase (complex i) (28 kDa, Protein from Yarrowia lipolytica)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (53 kDa, Protein from Candida lipolytica)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Subunit nuxm of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Candida lipolytica)
- Acyl carrier protein acpm1 of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Candida lipolytica)
- Nadh dehydrogenase subunit 2 (53 kDa, Protein from Yarrowia lipolytica)
- Subunit nukm of nadh:ubiquinone oxidoreductase (complex i) (23 kDa, Protein from Candida lipolytica)
- Nadh-ubiquinone oxidoreductase 78 kda subunit (79 kDa, Protein from Candida lipolytica)
- Nadh-ubiquinone oxidoreductase chain 5 (73 kDa, Protein from Yarrowia lipolytica)
- Subunit nuhm of nadh:ubiquinone oxidoreductase (complex i) (27 kDa, Protein from Candida lipolytica)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Subunit ni9m of protein nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Subunit nuim of nadh:ubiquinone oxidoreductase (complex i) (25 kDa, Protein from Candida lipolytica)
- Subunit nupm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Subunit nuum of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (20 kDa, Protein from Candida lipolytica)
- Subunit nufm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Subunit nb2m of nadh:ubiquinone oxidoreductase (complex i) (6 kDa, Protein from Yarrowia lipolytica)
- Subunit nb6m of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Subunit n7bm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase 40 kda subunit (42 kDa, Protein from Candida lipolytica)
- Subunit nidm of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Candida lipolytica)
- Subunit ni8m of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Candida lipolytica)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Yarrowia lipolytica)
Helicobacter pylori urease with BME bound in the active site
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 6qsu
Q-score: 0.656
Cunha ES, Chen X, Sanz-Gaitero M, Mills DJ, Luecke H
Nat Commun (2021) 12 pp. 230-230 [ Pubmed: 33431861 DOI: doi:10.1038/s41467-020-20485-6 ]
- Urease subunit alpha (26 kDa, Protein from Helicobacter pylori)
- Nickel (ii) ion (58 Da, Ligand)
- Beta-mercaptoethanol (78 Da, Ligand)
- 1.1 mda helicobacter pylori urease (1 MDa, Complex from Helicobacter pylori)
- Water (18 Da, Ligand)
- Urease subunit beta (61 kDa, Protein from Helicobacter pylori)
Heterotetrameric structure of the rBAT-b(0,+)AT1 complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6yup
Q-score: 0.491
Wu D, Grund TN, Welsch S, Mills DJ, Michel M, Safarian S, Michel H
PNAS (2020) 117 pp. 21281-21287 [ DOI: doi:10.1073/pnas.2008111117 Pubmed: 32817565 ]
- Calcium ion (40 Da, Ligand)
- B(0,+)-type amino acid transporter 1 (53 kDa, Protein from Homo sapiens)
- Heterotetrameric complex of rbat and b(0,+)at1 (264 MDa, Complex from Homo sapiens)
- Neutral and basic amino acid transport protein rbat (78 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Homodimeric structure of the rBAT complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6yuz
Q-score: 0.56
Wu D, Grund TN, Welsch S, Mills DJ, Michel M, Safarian S, Michel H
PNAS (2020) 117 pp. 21281-21287 [ DOI: doi:10.1073/pnas.2008111117 Pubmed: 32817565 ]
- Neutral and basic amino acid transport protein rbat (78 kDa, Protein from Homo sapiens)
- Homodimeric complex of rbat (157 MDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)