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SpCas9 bound to 18 nucleotide complementary DNA substrate in the checkpoint state
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
Human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif
Clerici M, Faini M, Muckenfuss LM, Aebersold R, Jinek M
Nat Struct Mol Biol (2018) 25 pp. 135-138 [ Pubmed: 29358758 DOI: 10.1038/s41594-017-0020-6 ]
Image sets:
CryoEM structure of the apo SPARTA (BabAgo/TIR-APAZ) complex
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 8qlo
Q-score: 0.505
Finocchio G, Koopal B, Potocnik A, Heijstek C, Westphal AH, Jinek M, Swarts DC
Nucleic Acids Res (2024) 52 pp. 2012-2029 [ Pubmed: 38224450 DOI: doi:10.1093/nar/gkad1248 ]
CryoEM structure of the RNA/DNA bound SPARTA (BabAgo/TIR-APAZ) tetrameric complex
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8qlp
Q-score: 0.391
Finocchio G, Koopal B, Potocnik A, Heijstek C, Westphal AH, Jinek M, Swarts DC
Nucleic Acids Res (2024) 52 pp. 2012-2029 [ Pubmed: 38224450 DOI: doi:10.1093/nar/gkad1248 ]
- Rna (5'-r(*ap*up*gp*ap*cp*gp*gp*cp*up*cp*up*ap*ap*up*cp*up*ap*up*up*ap*gp*u)-3') (6 kDa, RNA from Bacillales bacterium)
- Short prokaryotic argonaute (57 kDa, Protein from Bacillales bacterium)
- Rna/dna bound sparta (babago/tir-apaz) tetrameric complex (Complex from Bacillales bacterium)
- Toll/interleukin-1 receptor domain-containing protein (53 kDa, Protein from Bacillales bacterium)
- Dna (5'-d(*ap*cp*tp*ap*ap*tp*ap*gp*ap*tp*tp*ap*gp*ap*gp*cp*cp*gp*tp*c)-3') (7 kDa, DNA from Bacillales bacterium)
- Magnesium ion (24 Da, Ligand)
SpCas9 bound to 6 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 12 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 14 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 16 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 18 nucleotide complementary DNA substrate in the catalytic state
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 10 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets: