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CryoEM Structure of E. coli Transcription-Coupled Ribonucleotide Excision Repair (TC-RER) complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7uwe
Q-score: 0.413
Hao Z, Gowder M, Proshkin S, Bharati BK, Epshtein V, Svetlov V, Shamovsky I, Nudler E
Cell (2023) 186 pp. 2425- [ DOI: doi:10.1016/j.cell.2023.04.029 Pubmed: 37196657 ]
- Rna (18-mer) (5 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Ribonuclease hii (21 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Elongation complex(ec)-rnaseh2 complex (Complex from Escherichia coli)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7uwh
Q-score: 0.458
Hao Z, Gowder M, Proshkin S, Bharati BK, Epshtein V, Svetlov V, Shamovsky I, Nudler E
Cell (2023) 186 pp. 2425- [ DOI: doi:10.1016/j.cell.2023.04.029 Pubmed: 37196657 ]
- Elongation complex(ec)-rnaseh2 complex bound to ribonucleotide substrate (Complex from Escherichia coli)
- Rna (18-mer) (5 kDa, RNA from Escherichia coli)
- Dna/rna (59-mer) (18 kDa, Macromolecule from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna (59-mer) (18 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Ribonuclease hii (21 kDa, Protein from Escherichia coli)
CryoEM structure of E.coli transcription elongation complex
Resolution: 2.42 Å
EM Method: Single-particle
Fitted PDBs: 8fvr
Q-score: 0.566
Weaver JW, Proshkin S, Duan W, Epshtein V, Gowder M, Bharati BK, Afanaseva E, Mironov A, Serganov A, Nudler E
Nat Struct Mol Biol (2023) 30 pp. 600-607 [ Pubmed: 36997761 DOI: doi:10.1038/s41594-023-00948-2 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Rna (16-mer) (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna (53-mer) (16 kDa, DNA from Escherichia coli)
- E. coli transcription elongation complex (ec) (Complex from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna (53-mer) (16 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
CryoEM structure of E.coli transcription elongation complex bound to ppGpp
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 8fvw
Q-score: 0.611
Weaver JW, Proshkin S, Duan W, Epshtein V, Gowder M, Bharati BK, Afanaseva E, Mironov A, Serganov A, Nudler E
Nat Struct Mol Biol (2023) 30 pp. 600-607 [ Pubmed: 36997761 DOI: doi:10.1038/s41594-023-00948-2 ]
- E. coli transcription elongation complex bound to ppgpp (ec-ppgpp) (Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Rna (16-mer) (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna (53-mer) (16 kDa, DNA from Escherichia coli)
- Guanosine-5',3'-tetraphosphate (603 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (53-mer) (16 kDa, DNA from Escherichia coli)
- Sodium ion (22 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)