Electron Microscopy Data Bank

EMD-1787

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Asymmetric reconstruction of doublecortin-stabilised microtubules decorated with kinesin motor domain

Release date: Oct. 29, 2010
Resolution: 13.5 Å
EM Method: Single-particle

Fourniol FJ, Sindelar CV, Amigues B, Clare DK, Thomas G, Perderiset M, Francis F, Houdusse A, Moores CA

J Cell Biol (2010) 191 pp. 463-470 [ DOI: doi:10.1083/jcb.201007081 Pubmed: 20974813 ]

Sample:

Kinesin motor domain (Protein from Rattus norvegicus)
Doublecortin (Protein from Homo sapiens)
Alpha-beta tubulin dimer (Protein from Bos taurus)
Microtubules co-polymerised with doublecortin and bound with kinesin motor domain (Sample)

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EMD-1788

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Doublecortin-stabilised microtubules at secondary structure resolution

Release date: Oct. 29, 2010
Resolution: 8.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 2xrp
Q-score: 0.068

Fourniol FJ, Sindelar CV, Amigues B, Clare DK, Thomas G, Perderiset M, Francis F, Houdusse A, Moores CA

J Cell Biol (2010) 191 pp. 463-470 [ DOI: doi:10.1083/jcb.201007081 Pubmed: 20974813 ]

Sample:

Tubulin beta-2b chain (Protein from Bos taurus)
Doublecortin (Protein from Homo sapiens)
Tubulin alpha-1d chain (Protein from Bos taurus)
Microtubules co-polymerised with doublecortin (Sample)

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EMD-5223

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Human Ndc80 Bonsai Decorated Microtubule

Release date: Oct. 4, 2010
Resolution: 8.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 3iz0
Q-score: 0.11

Alushin GM, Ramey VH, Pasqualato S, Ball DA, Grigorieff N, Musacchio A, Nogales E

Nature (2010) 467 pp. 805-810 [ DOI: doi:10.1038/nature09423 Pubmed: 20944740 ]

Sample:

Tubulin (110 kDa, Protein from Bos taurus)
Human ndc80 bonsai complex bound to the microtubule (260 kDa, Sample)
Ndc80-spc25 chimera (45 kDa, Protein from Homo sapiens)
Nuf2-spc24 chimera (29 kDa, Protein from Homo sapiens)

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EMD-1701

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The molecular mechanism of the multi-tasking kinesin-8 motor

Release date: Oct. 1, 2010
Resolution: 10.1 Å
EM Method: Helical reconstruction

Peters C, Brejc K, Belmont L, Bodey AJ, Lee Y, Yu M, Guo J, Sakowicz R, Hartman J, Moores CA

EMBO J (2010) 29 pp. 3437-3447 [ DOI: doi:10.1038/emboj.2010.220 Pubmed: 20818331 ]

Sample:

Microtubule complexed with motor domain of kif18a (no nucleotide state) (Sample)
Motor domain of kif18a (Protein from Homo sapiens)
Beta tubulin (Protein from Bos taurus)
Alpha tubulin (Protein from Bos taurus)

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EMD-1702

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Kif18A (ATP state) head bound to a microtubule

Release date: Oct. 1, 2010
Resolution: 13.0 Å
EM Method: Helical reconstruction

Peters C, Brejc K, Belmont L, Bodey AJ, Lee Y, Yu M, Guo J, Sakowicz R, Hartman J, Moores CA

EMBO J (2010) 29 pp. 3437-3447 [ DOI: doi:10.1038/emboj.2010.220 Pubmed: 20818331 ]

Sample:

Beta tubulin (Protein from Bos taurus)
Motor domain of kif18a (Protein from Homo sapiens)
Microtubule complexed with motor domain of kif18a (atp state) (Sample)
Alpha tubulin (Protein from Bos taurus)

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EMD-5192

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Structural map of MT 12-3

Release date: Sept. 1, 2010
Resolution: 9.0 Å
EM Method: Single-particle

Sui H, Downing KH

Structure (2010) 18 pp. 1022-1031 [ Pubmed: 20696402 DOI: doi:10.1016/j.str.2010.05.010 ]

Sample:

Microtubule with 12 protofilaments (50 MDa, Protein from Bos taurus)
The microtubule containing 12 protofilaments (Sample)

EMD-5193

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Structural map of MT 13-3

Release date: Sept. 1, 2010
Resolution: 9.8 Å
EM Method: Single-particle

Sui H, Downing KH

Structure (2010) 18 pp. 1022-1031 [ Pubmed: 20696402 DOI: doi:10.1016/j.str.2010.05.010 ]

Sample:

The microtubule containing 13 protofilaments (Sample)
Microtubule with 13 protofilaments (50 MDa, Protein from Bos taurus)

EMD-5194

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Structural map of MT 14-3

Release date: Sept. 1, 2010
Resolution: 9.3 Å
EM Method: Single-particle

Sui H, Downing KH

Structure (2010) 18 pp. 1022-1031 [ Pubmed: 20696402 DOI: doi:10.1016/j.str.2010.05.010 ]

Sample:

The microtubule containing 14 protofilaments (Sample)
Microtubule with 14 protofilaments (50 MDa, Protein from Bos taurus)

EMD-5195

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Structural map of MT 15-4

Release date: Sept. 1, 2010
Resolution: 8.9 Å
EM Method: Single-particle

Sui H, Downing KH

Structure (2010) 18 pp. 1022-1031 [ Pubmed: 20696402 DOI: doi:10.1016/j.str.2010.05.010 ]

Sample:

The microtubule containing 15 protofilaments (Sample)
Microtubule with 15 protofilaments (50 MDa, Protein from Bos taurus)

EMD-5191

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Structural map of MT 11-3

Release date: Aug. 31, 2010
Resolution: 10.0 Å
EM Method: Single-particle

Sui H, Downing KH

Structure (2010) 18 pp. 1022-1031 [ Pubmed: 20696402 DOI: doi:10.1016/j.str.2010.05.010 ]

Sample:

Microtubule with 11 protofilaments (50 MDa, Protein from Bos taurus)
The microtubule with 11 protofilaments (Sample)

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