KS + AT di-domain of polyketide synthase 13 in Mycobacterium tuberculosis
Johnston HE, Batt SM, Brown AK, Savva CG, Besra GS, Futterer K
To Be Published
- Polyketide synthase pks13 (186 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Subunit of pks13 (187 kDa, Complex from Mycobacterium tuberculosis)
Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the pre-translocation state
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8j5q
Q-score: 0.519
Yang X, Hu T, Liang J, Xiong Z, Lin Z, Zhao Y, Zhou X, Gao Y, Sun S, Yang X, Guddat LW, Yang H, Rao Z, Zhang B
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01256-z Pubmed: 38548954 ]
- Uncharacterized abc transporter atp-binding protein rv1281c (65 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Cryo-em structure of mycobacterium tuberculosis oppabcd in the pre-translocation state (Complex from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Uncharacterized protein rv1280c (64 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Endogenous oligopeptide (783 Da, Protein from Mycolicibacterium smegmatis MC2 155)
- Iron/sulfur cluster (351 Da, Ligand)
- Putative peptide transport permease protein rv1283c (35 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Putative peptide transport permease protein rv1282c (31 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the resting state
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 8j5r
Q-score: 0.534
Yang X, Hu T, Liang J, Xiong Z, Lin Z, Zhao Y, Zhou X, Gao Y, Sun S, Yang X, Guddat LW, Yang H, Rao Z, Zhang B
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01256-z Pubmed: 38548954 ]
- Uncharacterized abc transporter atp-binding protein rv1281c (65 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Uncharacterized protein rv1280c (64 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Iron/sulfur cluster (351 Da, Ligand)
- Putative peptide transport permease protein rv1283c (35 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Cryo-em structure of mycobacterium tuberculosis oppabcd in the resting state (Complex from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Putative peptide transport permease protein rv1282c (31 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the pre-catalytic intermediate state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8j5s
Q-score: 0.566
Yang X, Hu T, Liang J, Xiong Z, Lin Z, Zhao Y, Zhou X, Gao Y, Sun S, Yang X, Guddat LW, Yang H, Rao Z, Zhang B
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01256-z Pubmed: 38548954 ]
- Cryo-em structure of mycobacterium tuberculosis oppabcd in the pre-catalytic intermediate state (Complex from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Uncharacterized protein rv1280c (64 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Endogenous oligopeptide (698 Da, Protein from Mycolicibacterium smegmatis MC2 155)
- Iron/sulfur cluster (351 Da, Ligand)
- Putative peptide transport permease protein rv1283c (35 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Uncharacterized abc transporter atp-binding protein rv1281c (65 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Magnesium ion (24 Da, Ligand)
- Putative peptide transport permease protein rv1282c (31 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the catalytic intermediate state
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8j5t
Q-score: 0.572
Yang X, Hu T, Liang J, Xiong Z, Lin Z, Zhao Y, Zhou X, Gao Y, Sun S, Yang X, Guddat LW, Yang H, Rao Z, Zhang B
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01256-z Pubmed: 38548954 ]
- Uncharacterized protein rv1280c (64 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Iron/sulfur cluster (351 Da, Ligand)
- Putative peptide transport permease protein rv1283c (35 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Uncharacterized abc transporter atp-binding protein rv1281c (65 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Magnesium ion (24 Da, Ligand)
- Cryo-em structure of mycobacterium tuberculosis oppabcd in the catalytic intermediate state (Complex from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Putative peptide transport permease protein rv1282c (31 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
Membrane bound PRTase, C3 symmetry, donor bound
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8j8j
Q-score: 0.601
Gao S, Wu F, Gurcha SS, Batt SM, Besra GS, Rao Z, Zhang L
Nat Microbiol (2024) 9 pp. 976-987 [ Pubmed: 38491273 DOI: doi:10.1038/s41564-024-01643-8 ]
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Trimeric phosphoribosyltransferase (97 kDa, Complex from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Magnesium ion (24 Da, Ligand)
- Decaprenyl-phosphate phosphoribosyltransferase (32 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 1-o-pyrophosphono-5-o-phosphono-alpha-d-ribofuranose (390 Da, Ligand)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8v9k
Q-score: 0.461
Helena-Bueno K, Rybak MY, Ekemezie CL, Sullivan R, Brown CR, Dingwall C, Basle A, Schneider C, Connolly JPR, Blaza JN, Csorgo B, Moynihan PJ, Gagnon MG, Hill CH, Melnikov SV
Nature (2024) 626 pp. 1125-1132 [ DOI: doi:10.1038/s41586-024-07041-8 Pubmed: 38355796 ]
- Small ribosomal subunit protein bs18b (9 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul22 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s7 (17 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul5 (21 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s17 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s13 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 5s ribosomal rna (38 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l24 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Zinc ion (65 Da, Ligand)
- Large ribosomal subunit protein ul23 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s4 (23 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl20 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l30 (7 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s10 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Poly-u mrna (873 Da, RNA from Mycolicibacterium smegmatis MC2 155)
- 23s ribosomal rna (1 MDa, RNA from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l25 (22 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l34 (5 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l6 (19 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s22 (4 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l35 (7 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l9 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 16s ribosomal rna (495 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- Ribosome hibernation factor balon (rv2629) (40 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Large ribosomal subunit protein ul18 (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s12 (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l36 (4 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l37 (2 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s2 (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Small ribosomal subunit protein us14b (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l17 (21 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l19 (12 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s19 (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l10 (18 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul1 (25 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s9 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s6 (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l15 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Cryo-em structure of the mycobacterium smegmatis 70s ribosome in complex with hibernation factor rv2629 (balon) (structure 5) (2 MDa, Complex from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l32 (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l27 (9 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s3 (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Pe/e deacylated phenylanaline-trna (24 kDa, RNA from Mycolicibacterium smegmatis MC2 155)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l2 (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl33a (6 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein bl31 (8 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s11 (14 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Small ribosomal subunit protein us5 (21 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l3 (23 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s16 (16 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l14 (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l11 (15 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 50s ribosomal protein l29 (8 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- 30s ribosomal protein s20 (9 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Cryo-EM structure of Mycobacterium tuberculosis LpqY-SugABC in complex with trehalose
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8ja7
Q-score: 0.428
Liang J, Liu F, Xu P, Shangguan W, Hu T, Wang S, Yang X, Xiong Z, Yang X, Guddat LW, Yu B, Rao Z, Zhang B
PNAS (2023) 120 pp. e2307625120-e2307625120 [ DOI: doi:10.1073/pnas.2307625120 Pubmed: 37603751 ]
- Trehalose import atp-binding protein sugc (42 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Trehalose transport system permease protein suga (33 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Trehalose transport system permease protein sugb (29 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Trehalose-binding lipoprotein lpqy (49 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Lpqy-sugabc (Complex from Mycobacterium tuberculosis H37Rv)
Zhang Y, van Schayck JP, Pedrazo-Tardajos A, Claes N, Noteborn WEM, Lu PH, Duimel H, Dunin-Borkowski RE, Bals S, Peters PJ, Ravelli RBG
ACS Nano (2023) 17 pp. 15836-15846 [ DOI: doi:10.1021/acsnano.3c03722 Pubmed: 37531407 ]