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Structure of the undecorated barbed end of F-actin.
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8ru0
Q-score: 0.542
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Phosphate ion (94 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin filament (Complex)
- Magnesium ion (24 Da, Ligand)
- Complex of the actin subunits that form the barbed end of actin filaments. (Complex from Oryctolagus cuniculus)
Structure of the formin INF2 bound to the barbed end of F-actin.
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rv2
Q-score: 0.43
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Inf2 dimer (Complex from Homo sapiens)
- Phosphate ion (94 Da, Ligand)
- Actin filament barbed end (Complex from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Isoform 2 of inverted formin-2 (84 kDa, Protein from Homo sapiens)
- Complex of the formin inf2 dimer that binds to the actin subunits at the barbed end of actin filaments. (Complex)
- Magnesium ion (24 Da, Ligand)
Atomic structure of Salmonella SipA/F-actin complex by cryo-EM
Resolution: 3.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 8uee
Q-score: 0.506
Niedzialkowska E, Runyan L, Kudryashova E, Kudryashov DS, Egelman EH
To Be Published
- Cell invasion protein sipa (28 kDa, Protein from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2)
- Phosphate ion (94 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Sipa (Complex)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Actin, alpha skeletal muscle (Complex)
- Salmonella sipa/f-actin complex (Complex from Oryctolagus cuniculus)
Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7t5q
Q-score: 0.385
Rebowski G, van Eeuwen T, Dominguez R, Boczkowska M
To Be Published
- Actin-related protein 2/3 complex subunit 1a (41 kDa, Protein from cattle)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from cattle)
- Actin-related protein 2 (44 kDa, Protein from cattle)
- F-actin-capping protein subunit beta/actin nucleation-promoting factor wasl chimera (37 kDa, Protein from Mus musculus)
- Actin-related protein 2/3 complex subunit 2 (34 kDa, Protein from cattle)
- Actin, alpha skeletal muscle (41 kDa, Protein from rabbit)
- Bovine arp2/3 complex and actin, capz (248 kDa, Complex)
- Actin-related protein 3 (47 kDa, Protein from cattle)
- F-actin-capping protein subunit alpha-1/actin nucleation-promoting factor wasl chimera (39 kDa, Protein from Mus musculus)
- Actin skeletal muscle (Complex from Oryctolagus cuniculus)
- F-actin-capping protein (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from cattle)
- Magnesium ion (24 Da, Ligand)
- Actin-related protein 2/3 complex subunit 3 (20 kDa, Protein from cattle)
- Bovine arp2/3 (Complex from Bos taurus)
Cryo-EM structure of F-actin in the ADP state
Resolution: 2.26 Å
EM Method: Helical reconstruction
Fitted PDBs: 8f8p
Q-score: 0.587
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- F-actin in the adp state (Complex from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of the CapZ-capped barbed end of F-actin
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8f8q
Q-score: 0.571
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- Capz (alpha and beta subunits) (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Capz-capped barbed end of f-actin (Complex)
- F-actin-capping protein subunit beta (31 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- F-actin-capping protein subunit alpha-1 (32 kDa, Protein from Homo sapiens)
- Actin filament (Complex from Oryctolagus cuniculus)
Cryo-EM structure of the free barbed end of F-actin
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8f8r
Q-score: 0.469
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- Free barbed end of f-actin (Complex from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of the free pointed end of F-actin
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8f8s
Q-score: 0.572
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- Free pointed end of f-actin (Complex from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of the Tropomodulin-capped pointed end of F-actin
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 8f8t
Q-score: 0.462
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tropomodulin (tmod) (Complex from Homo sapiens)
- Tropomodulin-1 (40 kDa, Protein from Homo sapiens)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Magnesium ion (24 Da, Ligand)
- Tropomodulin-capped pointed end of f-actin (Complex)
- Actin filament (Complex from Oryctolagus cuniculus)
Cryo-EM structure of skeletal muscle alpha-actin
Resolution: 3.37 Å
EM Method: Helical reconstruction
Fitted PDBs: 8dmx
Q-score: 0.52
Arora AS, Huang HL, Singh R, Narui Y, Suchenko A, Hatano T, Heissler SM, Balasubramanian MK, Chinthalapudi K
eLife (2023) 12 [ DOI: doi:10.7554/eLife.82015 Pubmed: 36790143 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin (Cellular component from Oryctolagus cuniculus)
- Actin, alpha skeletal muscle (41 kDa, Protein from rabbit)
- Magnesium ion (24 Da, Ligand)