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Structure of the undecorated barbed end of F-actin.
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8ru0
Q-score: 0.542
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Complex of the actin subunits that form the barbed end of actin filaments. (Complex from Oryctolagus cuniculus)
- Actin filament (Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
Structure of the formin INF2 bound to the barbed end of F-actin.
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rv2
Q-score: 0.43
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Inf2 dimer (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of the formin inf2 dimer that binds to the actin subunits at the barbed end of actin filaments. (Complex)
- Actin filament barbed end (Complex from Oryctolagus cuniculus)
- Isoform 2 of inverted formin-2 (84 kDa, Protein from Homo sapiens)
Cryo-EM structure of F-actin in the ADP state
Resolution: 2.26 Å
EM Method: Helical reconstruction
Fitted PDBs: 8f8p
Q-score: 0.587
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ Pubmed: 37228182 DOI: doi:10.1126/science.adg6812 ]
- F-actin in the adp state (Complex from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
Cryo-EM structure of the CapZ-capped barbed end of F-actin
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8f8q
Q-score: 0.571
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ Pubmed: 37228182 DOI: doi:10.1126/science.adg6812 ]
- Actin filament (Complex from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Capz (alpha and beta subunits) (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- F-actin-capping protein subunit alpha-1 (32 kDa, Protein from Homo sapiens)
- Capz-capped barbed end of f-actin (Complex)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- F-actin-capping protein subunit beta (31 kDa, Protein from Homo sapiens)
Cryo-EM structure of the free barbed end of F-actin
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8f8r
Q-score: 0.469
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ Pubmed: 37228182 DOI: doi:10.1126/science.adg6812 ]
- Free barbed end of f-actin (Complex from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
Cryo-EM structure of the free pointed end of F-actin
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8f8s
Q-score: 0.572
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ Pubmed: 37228182 DOI: doi:10.1126/science.adg6812 ]
- Free pointed end of f-actin (Complex from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
Cryo-EM structure of the Tropomodulin-capped pointed end of F-actin
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 8f8t
Q-score: 0.462
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ Pubmed: 37228182 DOI: doi:10.1126/science.adg6812 ]
- Actin filament (Complex from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tropomodulin (tmod) (Complex from Homo sapiens)
- Tropomodulin-capped pointed end of f-actin (Complex)
- Magnesium ion (24 Da, Ligand)
- Tropomodulin-1 (40 kDa, Protein from Homo sapiens)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
In situ structure of myosin neck domain in skeletal sarcomere (centered on essential light chain)
In situ structure of myosin double-head in cardiac sarcomere
In situ structure of actomyosin complex in skeletal sarcomere
Resolution: 6.6 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7qin
Q-score: 0.196
Wang Z, Grange M, Pospich S, Wagner T, Kho AL, Gautel M, Raunser S
Science (2022) 375 pp. eabn1934-eabn1934 [ Pubmed: 35175800 DOI: doi:10.1126/science.abn1934 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tropomyosin, alpha-1 (mouse) (7 kDa, Protein from house mouse)
- Magnesium ion (24 Da, Ligand)
- Tropomyosin, alpha-1 (mouse) (10 kDa, Protein from house mouse)
- Actin, alpha skeletal muscle (41 kDa, Protein from house mouse)
- Myosin-4 (96 kDa, Protein from house mouse)
- Tropomyosin, alpha-1 (mouse) (9 kDa, Protein from house mouse)
- Mouse psoas muscle myofibrils (Cellular component from Mus musculus)
- Nebulin (mouse) (6 kDa, Protein from house mouse)
- Nebulin (mouse) (9 kDa, Protein from house mouse)