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Acto-HMM complex in ADP-state. Chicken smooth muscle HMM and chicken pectoralis actin
Hojjatian A, Taylor DW, Daneshparvar N, Fagnant PM, Trybus KM, Taylor KA
J Struct Biol (2023) 215 pp. 107995-107995 [ DOI: doi:10.1016/j.jsb.2023.107995 Pubmed: 37414375 ]
- Myosin light polypeptide 6 (16 kDa, Protein from Gallus gallus)
- Myosin-11 (97 kDa, Protein from Gallus gallus)
- Myosin regulatory light chain 2, smooth muscle major isoform (16 kDa, Protein from Gallus gallus)
- Actin, alpha skeletal muscle (41 kDa, Protein from Gallus gallus)
- Acto-hmm complex - chicken smooth muscle heavy meromyosin in complex with chicken pectoralis muscle actin filament (Complex from Gallus gallus)
CryoEM map of myosin thick filament from Bombus ignitus asynchronous flight muscle
Li J, Rahmani H, Abbasi Yeganeh F, Rastegarpouyani H, Taylor DW, Wood NB, Previs MJ, Iwamoto H, Taylor KA
Int J Mol Sci (2022) 24 [ DOI: doi:10.3390/ijms24010377 Pubmed: 36613818 ]
The Myosin II Coiled Coil Domain Atomic Structure in its Native Environment
Rahmani H, Ma W, Hu Z, Daneshparvar N, Taylor DW, McCammon JA, Irving TC, Edwards RJ, Taylor KA
Proc Natl Acad Sci U S A (2021) 118 [ DOI: 10.1073/pnas.2024151118 Pubmed: 33782130 ]
Image sets:
- Particle Stack
- Frame Stacks 2/3
- Micrographs before frames alignment
- Micrographs: frame-aligned and dose-weighted
- Frame Stacks 3/3
- Frame Stacks 1/3
Lethocerus Myosin II complete coiled-coil domain resolved in its native environment
Resolution: 4.25 Å
EM Method: Single-particle
Fitted PDBs: 7kog
Q-score: 0.044
Rahmani H, Ma W, Hu Z, Daneshparvar N, Taylor DW, McCammon JA, Irving TC, Edwards RJ, Taylor KA
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2024151118 Pubmed: 33782130 ]
The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State
Resolution: 6.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 6bih
Q-score: 0.133
Banerjee C, Hu Z, Huang Z, Warrington JA, Taylor DW, Trybus KM, Lowey S, Taylor KA
J Struct Biol (2017) 200 pp. 325-333 [ DOI: doi:10.1016/j.jsb.2017.10.003 Pubmed: 29038012 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Filamentous muscle alpha-actin decorated with the motor domain of recombinant smooth muscle myosin ii motor domain expressed in sf9 cells (Complex)
- Magnesium ion (24 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from Oryctolagus cuniculus)
- Alpha-actin (Complex from Oryctolagus cuniculus)
- Myosin-11 (91 kDa, Protein from Gallus gallus)
- Myosin ii (Complex from Gallus gallus)
Hu Z, Taylor DW, Edwards RJ, Taylor KA
J Struct Biol (2017) 200 pp. 334-342 [ Pubmed: 28964844 DOI: doi:10.1016/j.jsb.2017.09.009 ]
The Structure of the Relaxed Thick Filaments from Lethocerus Flight Muscle
Hu Z, Taylor DW, Reedy MK, Perz-Edwards RJ, Taylor KA
Sci Adv (2016) 2 [ Pubmed: 27704041 DOI: doi:10.1126/sciadv.1600058 ]
- Myofilin (30 kDa, Protein from Lethocerus indicus)
- Thick filament from lethocerus (waterbug) flight muscle (Sample)
- Paramyosin (100 kDa, Protein from Lethocerus indicus)
- Flightin (19 kDa, Protein from Lethocerus indicus)
- Myosin ii (520 kDa, Protein from Lethocerus indicus)
Phosphorylated smooth muscle heavy meromyosin shows an open conformation linked to activation
Resolution: 20.0 Å
EM Method: Electron Crystallography
Fitted PDBs: 3j04
Q-score: 0.033
Baumann BA, Taylor DW, Huang Z, Tama F, Fagnant PM, Trybus KM, Taylor KA
J Mol Biol (2012) 415 pp. 274-287 [ Pubmed: 22079364 DOI: doi:10.1016/j.jmb.2011.10.047 ]
Three-dimensional structure of the myosin V inhibited state by cryoelectron tomography.
Resolution: 24.0 Å
EM Method: Subtomogram averaging
Fitted PDBs: 2dfs
Q-score: 0.045
Liu J, Taylor DW, Krementsova EB, Trybus KM, Taylor KA
Nature (2006) 442 pp. 208-211 [ DOI: doi:10.1038/nature04719 Pubmed: 16625208 ]
- Myosin v (400 kDa, Sample)
- Myosin-v (400 kDa, Protein from Mus musculus)