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Cryo-EM structure of a respiratory complex I mutant lacking NDUFS4
Resolution: 4.04 Å
EM Method: Single-particle
Fitted PDBs: 6rfs
Q-score: 0.342
Parey K, Haapanen O, Sharma V, Kofeler H, Zullig T, Prinz S, Siegmund K, Wittig I, Mills DJ, Vonck J, Kuhlbrandt W, Zickermann V
Sci Adv (2019) 5 pp. eaax9484-eaax9484 [ DOI: doi:10.1126/sciadv.aax9484 Pubmed: 31844670 ]
- Subunit nugm of nadh:ubiquinone oxidoreductase (complex i) (32 kDa, Protein from Candida lipolytica)
- Acyl carrier protein acpm1 of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Candida lipolytica)
- Subunit nuzm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Subunit nunm of nadh:ubiquinone oxidoreductase (complex i) (13 kDa, Protein from Yarrowia lipolytica)
- Subunit nubm of nadh:ubiquinone oxidoreductase (complex i) (53 kDa, Protein from Candida lipolytica)
- Subunit nipm of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Candida lipolytica)
- Subunit numm of nadh:ubiquinone oxidoreductase (complex i) (15 kDa, Protein from Candida lipolytica)
- Subunit nucm of nadh:ubiquinone oxidoreductase (complex i) (52 kDa, Protein from Candida lipolytica)
- Iron/sulfur cluster (351 Da, Ligand)
- Subunit nesm of nadh:ubiquinone oxidoreductase (complex i) (28 kDa, Protein from Yarrowia lipolytica)
- Subunit nb4m of protein nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Subunit nu3m of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Yarrowia lipolytica)
- Subunit nebm of nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Zinc ion (65 Da, Ligand)
- Mitochondrial nadh:ubiquinone oxidoreductase (1 MDa, Complex from Yarrowia lipolytica)
- Subunit nb8m of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Candida lipolytica)
- Subunit nu6m of nadh:ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Yarrowia lipolytica)
- Subunit nulm of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Subunit nuhm of nadh:ubiquinone oxidoreductase (complex i) (27 kDa, Protein from Candida lipolytica)
- Subunit nukm of nadh:ubiquinone oxidoreductase (complex i) (23 kDa, Protein from Candida lipolytica)
- Subunit ni2m of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Candida lipolytica)
- Subunit nu4m of nadh:ubiquinone oxidoreductase (complex i) (54 kDa, Protein from Yarrowia lipolytica)
- Subunit nupm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Subunit nuvm of nadh:ubiquinone oxidoreductase (complex i) (7 kDa, Protein from Yarrowia lipolytica)
- Subunit nujm of nadh:ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Yarrowia lipolytica)
- Acyl carrier protein acpm2 of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Subunit nimm of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Candida lipolytica)
- Subunit nu1m of nadh:ubiquinone oxidoreductase (complex i) (38 kDa, Protein from Yarrowia lipolytica)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Subunit nuem of nadh:ubiquinone oxidoreductase (complex i) (42 kDa, Protein from Yarrowia lipolytica)
- Subunit niam of nadh:ubiquinone oxidoreductase (complex i) (17 kDa, Protein from Yarrowia lipolytica)
- Subunit nb5m of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Candida lipolytica)
- Subunit nidm of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Candida lipolytica)
- Subunit nuam of nadh:ubiquinone oxidoreductase (complex i) (79 kDa, Protein from Candida lipolytica)
- Subunit nu5m of nadh:ubiquinone oxidoreductase (complex i) (73 kDa, Protein from Yarrowia lipolytica)
- Subunit nuim of nadh:ubiquinone oxidoreductase (complex i) (25 kDa, Protein from Candida lipolytica)
- Subunit nuum of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Subunit nu2m of nadh:ubiquinone oxidoreductase (complex i) (53 kDa, Protein from Yarrowia lipolytica)
- Subunit ni9m of nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Subunit nb2m of nadh:ubiquinone oxidoreductase (complex i) (6 kDa, Protein from Yarrowia lipolytica)
- Subunit nufm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit nb6m of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Subunit n7bm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit ni8m of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Candida lipolytica)
- Subunit nuxm of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Candida lipolytica)
Respiratory complex I of Yarrowia lipolytica
D'Imprima E, Mills DJ, Parey K, Brandt U, Kuhlbrandt W, Zickermann V, Vonck J
Biochim Biophys Acta (2016) 1857 pp. 1935-1942 [ DOI: doi:10.1016/j.bbabio.2016.09.014 Pubmed: 27693469 ]
- Nadh:ubiquinone oxidoreductase (1 MDa, Complex from Yarrowia lipolytica)
Respiratory complex I of Yarrowia lipolytica with sulfur transferase subunit ST1 bound
D'Imprima E, Mills DJ, Parey K, Brandt U, Kuhlbrandt W, Zickermann V, Vonck J
Biochim Biophys Acta (2016) 1857 pp. 1935-1942 [ DOI: doi:10.1016/j.bbabio.2016.09.014 Pubmed: 27693469 ]
- Nadh:ubiquinone oxidoreductase (1 MDa, Complex from Yarrowia lipolytica)