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Structural organization of a late activated human spliceosome (Baqr, core region)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qtt
Q-score: 0.384
Schmitzova J, Cretu C, Dienemann C, Urlaub H, Pena V
Nature (2023) 617 pp. 842-850 [ DOI: doi:10.1038/s41586-023-06049-w Pubmed: 37165190 ]
- Splicing factor 3a subunit 2 (49 kDa, Protein from human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- G-patch domain and kow motifs-containing protein (52 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Late human activated spliceosome arrested with a dominant-negative mutant of the splicing helicase aquarius (aquarius k829a) (Complex from Homo sapiens)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Bud13 homolog (70 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from human)
- Minx (102 kDa, RNA from unidentified adenovirus)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from human)
- Ring finger protein 113a (38 kDa, Protein from human)
- U5 snrna (37 kDa, RNA from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 4 (57 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from human)
- U6snrna (34 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from human)
- U2snrna (60 kDa, RNA from human)
- Magnesium ion (24 Da, Ligand)
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 8ch6
Q-score: 0.154
Schmitzova J, Cretu C, Dienemann C, Urlaub H, Pena V
Nature (2023) 617 pp. 842-850 [ DOI: doi:10.1038/s41586-023-06049-w Pubmed: 37165190 ]
- Splicing factor 3a subunit 2 (49 kDa, Protein from human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- G-patch domain and kow motifs-containing protein (52 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from human)
- Late human activated spliceosome arrested with a dominant-negative mutant of the splicing helicase aquarius (aquarius k829a) (Complex from Homo sapiens)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Bud13 homolog (70 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Rnu6-1 (34 kDa, RNA from Homo sapiens)
- Minx-m3 (102 kDa, RNA from unidentified adenovirus)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Rnu5a-1 (37 kDa, RNA from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from human)
- Ring finger protein 113a (38 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Intron-binding protein aquarius (171 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 4 (57 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Rnu2-1 (60 kDa, RNA from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Splicing factor 3a subunit 1 (88 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
The cryo-EM structure of the human pre-A complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7vpx
Q-score: 0.155
Zhang X, Zhan X, Bian T, Yang F, Li P, Lu Y, Xing Z, Fan R, Zhang QC, Shi Y
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-023-01188-0 Pubmed: 38196034 ]
- Probable atp-dependent rna helicase ddx46 (117 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- U1 small nuclear ribonucleoprotein a (31 kDa, Protein from Homo sapiens)
- U1 snrna (52 kDa, RNA from Homo sapiens)
- Dnaj homolog subfamily c member 8 (29 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Spliceostatin a (form ii) (523 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- 5ss (3 kDa, RNA from unidentified adenovirus)
- Pre-mrna, 5ss (Complex)
- Human pre-a complex (Complex from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Pre-mrna (83 kDa, RNA from unidentified adenovirus)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Human pre-a (Complex from Homo sapiens)
- U1 small nuclear ribonucleoprotein 70 kda (51 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- U1 small nuclear ribonucleoprotein c (17 kDa, Protein from Homo sapiens)
- Splicing factor 1 (68 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
The cryo-EM structure of human pre-C*-I complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7w59
Q-score: 0.268
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ Pubmed: 35705093 DOI: doi:10.1016/j.molcel.2022.05.021 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Protein bud31 homolog (17 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Rna helicase aquarius (171 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from Human)
- U2 snrna (60 kDa, RNA from human)
- U5 snrna (37 kDa, RNA from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Psme3-interacting protein (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Human pre-c*-i complex (Complex from Homo sapiens)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
The cryo-EM structure of human pre-C*-II complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7w5a
Q-score: 0.269
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ Pubmed: 35705093 DOI: doi:10.1016/j.molcel.2022.05.021 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Protein bud31 homolog (17 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Rna helicase aquarius (171 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Human pre-c*-ii complex (Complex from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- U5 snrna (37 kDa, RNA from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Psme3-interacting protein (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Cactin (88 kDa, Protein from human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
The cryo-EM structure of human C* complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7w5b
Q-score: 0.192
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ Pubmed: 35705093 DOI: doi:10.1016/j.molcel.2022.05.021 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Protein bud31 homolog (17 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Rna helicase aquarius (171 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- U5 snrna (37 kDa, RNA from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Cactin (88 kDa, Protein from human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Nf-kappa-b-activating protein (47 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
- Human pre-c*-i complex (Complex from Homo sapiens)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
cryo-EM structure of a human activated spliceosome (mature Bact) at 5.1 angstrom.
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 5z56
Q-score: 0.131
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Human activated spliceosome (Complex from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Ring finger protein 113a (38 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
Cryo-EM structure of the human activated spliceosome (late Bact) at 6.5 angstrom
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 5z57
Q-score: 0.097
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Alanine (89 Da, Ligand)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Late bact splieosome (Complex from Homo sapiens)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 5z58
Q-score: 0.131
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Early bact spliceosome (Complex from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Ring finger protein 113a (38 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)