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Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8fx5
Q-score: 0.595
Burger WAC, Pham V, Vuckovic Z, Powers AS, Mobbs JI, Laloudakis Y, Glukhova A, Wootten D, Tobin AB, Sexton PM, Paul SM, Felder CC, Danev R, Dror RO, Christopoulos A, Valant C, Thal DM
Nat Commun (2023) 14 pp. 5440-5440 [ DOI: doi:10.1038/s41467-023-41199-5 Pubmed: 37673901 ]
- Xanomeline (281 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Mus musculus)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist xanomeline in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (26 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8f0j
Q-score: 0.621
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ DOI: doi:10.1038/s41589-023-01393-4 Pubmed: 37537379 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- Calcitonin receptor in complex with gs protein and san45 peptide (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Pramlintide analogue san45 (3 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
Resolution: 1.9 Å
EM Method: Single-particle
Fitted PDBs: 8f0k
Q-score: 0.564
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ DOI: doi:10.1038/s41589-023-01393-4 Pubmed: 37537379 ]
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- San385 (3 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Human amylin 3 receptor in complex with gs protein and san385 peptide (Complex from Homo sapiens)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Water (18 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8f2a
Q-score: 0.556
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ DOI: doi:10.1038/s41589-023-01393-4 Pubmed: 37537379 ]
- Human amylin 3 receptor in complex with gs protein and san385 peptide in another conformation (cluster 5) (Complex from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Pramlintide analogue san385 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8f2b
Q-score: 0.556
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ DOI: doi:10.1038/s41589-023-01393-4 Pubmed: 37537379 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Pramlintide analogue san45 (3 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Amylin 3 receptor in complex with gs protein and san45 peptide (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Human M4 muscarinic acetylcholine receptor complex with Gi1 and the agonist iperoxo
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7trk
Q-score: 0.548
eLife (2023) [ ]
- M4 machr bound to agonist iperoxo in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7trp
Q-score: 0.561
eLife (2023) [ ]
- M4 machr bound to agonist iperoxo and the pam ly2033298 in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-6-methoxy-4-methylthieno[2,3-b]pyridine-2-carboxamide (311 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7trq
Q-score: 0.587
eLife (2023) [ ]
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist iperoxo and the pam vu0467154 in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 5-amino-3,4-dimethyl-n-{[4-(trifluoromethanesulfonyl)phenyl]methyl}thieno[2,3-c]pyridazine-6-carboxamide (444 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Human M4 muscarinic acetylcholine receptor complex with Gi1 and the endogenous agonist acetylcholine
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7trs
Q-score: 0.503
eLife (2023) [ ]
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist acetylcholine in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16. (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of human nucleosome core particle composed of the Widom 601L DNA sequence
Resolution: 1.89 Å
EM Method: Single-particle
Fitted PDBs: 7vz4
Q-score: 0.724
Takizawa Y, Ho CH, Sato S, Danev R, Kurumizaka H
To Be Published
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (14 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Human nucleosome core particle composed of the widom 601l dna sequence (Complex from Homo sapiens)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)