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Resolution: 4.09 Å
EM Method: Single-particle
Fitted PDBs: 8a43
Q-score: 0.337
Daiss JL, Pilsl M, Straub K, Bleckmann A, Hocherl M, Heiss FB, Abascal-Palacios G, Ramsay EP, Tluckova K, Mars JC, Furtges T, Bruckmann A, Rudack T, Bernecky C, Lamour V, Panov K, Vannini A, Moss T, Engel C
Life Sci Alliance (2022) 5 [ DOI: doi:10.26508/lsa.202201568 Pubmed: 36271492 ]
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa34 (55 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa1 (195 kDa, Protein from Homo sapiens)
- Rna polymerase i (Complex from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa49 (47 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa2 (128 kDa, Protein from Homo sapiens)
Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC3
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 5oik
Q-score: 0.373
Bernecky C, Plitzko JM, Cramer P
Nat Struct Mol Biol (2017) 24 pp. 809-815 [ Pubmed: 28892040 DOI: doi:10.1038/nsmb.3465 ]
- Transcription elongation factor spt5 (121 kDa, Protein from Homo sapiens)
- Polymerase (rna) ii (dna directed) polypeptide d (16 kDa, Protein from Bos taurus)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Bos taurus)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Bos taurus)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Bos taurus)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Bos taurus)
- Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (134 kDa, Protein from Bos taurus)
- Rna polymerase ii (520 kDa, Complex from Bos taurus)
- Dsif (130 kDa, Complex from Homo sapiens)
- Dna-rna synthetic construct (40 kDa, Complex from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Bos taurus)
- Transcription elongation factor spt4 (13 kDa, Protein from Homo sapiens)
- Dna (43-mer) (13 kDa, DNA from synthetic construct)
- Uncharacterized protein (17 kDa, Protein from Bos taurus)
- Dna (43-mer) (13 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Bos taurus)
- Zinc ion (65 Da, Ligand)
- Rna polymerase ii-dsif elongation complex (690 kDa, Complex)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Bos taurus)
- Rna (5'-r(p*up*ap*up*ap*up*ap*cp*ap*up*ap*ap*ap*gp*ap*cp*cp*ap*gp*gp*c)-3') (16 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Bos taurus)
RNA polymerase I initially transcribing complex with visible tandem Winged Helix domain
Resolution: 6.9 Å
EM Method: Single-particle
Fitted PDBs: 5n61
Q-score: 0.139
Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky C, Cramer P
Cell (2017) 169 pp. 120-131.e22 [ DOI: doi:10.1016/j.cell.2017.03.003 Pubmed: 28340337 ]
- Rna polymerase i (590 kDa, Complex from Saccharomyces cerevisiae)
- Rna polymerase i initially transcribing complex (Complex)
- Initial transcription dna/rna scaffold (60 kDa, Complex from Saccharomyces cerevisiae)
- Initiation factor rrn3 (70 kDa, Complex from Saccharomyces cerevisiae)
- Core factor (220 kDa, Complex from Saccharomyces cerevisiae)
RNA polymerase I initially transcribing complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5n61
Q-score: 0.343
Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky C, Cramer P
Cell (2017) 169 pp. 120-131.e22 [ DOI: doi:10.1016/j.cell.2017.03.003 Pubmed: 28340337 ]
- Dna-directed rna polymerase i subunit rpa49 (46 kDa, Protein from Baker's yeast)
- Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Baker's yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Baker's yeast)
- Rna polymerase i-specific transcription initiation factor rrn6 (102 kDa, Protein from Saccharomyces cerevisiae)
- Initiation factor rrn3 (70 kDa, Complex from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Baker's yeast)
- Rna polymerase i-specific transcription initiation factor rrn7 (60 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Baker's yeast)
- Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Baker's yeast)
- Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Baker's yeast)
- Rna polymerase i-specific transcription initiation factor rrn11 (59 kDa, Protein from Saccharomyces cerevisiae)
- Rna polymerase i-specific transcription initiation factor rrn3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Baker's yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Baker's yeast)
- Sulfate ion (96 Da, Ligand)
- Initial transcription dna/rna scaffold (60 kDa, Complex from Saccharomyces cerevisiae)
- Non-template dna (12 kDa, DNA from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Baker's yeast)
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Baker's yeast)
- Template dna (13 kDa, DNA from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Baker's yeast)
- Core factor (220 kDa, Complex from Saccharomyces cerevisiae)
- Rna polymerase i (590 kDa, Complex from Saccharomyces cerevisiae)
- Product rna (1 kDa, RNA from Saccharomyces cerevisiae)
- Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand from Saccharomyces cerevisiae)
- Rna polymerase i initially transcribing complex (950 kDa, Complex)
- Dna-directed rna polymerase i subunit rpa34 (26 kDa, Protein from Baker's yeast)
Structure of transcribing mammalian RNA polymerase II (EC2)
Bernecky C, Herzog F, Baumeister W, Plitzko JM, Cramer P
Nature (2016) 529 pp. 551-554 [ Pubmed: 26789250 DOI: doi:10.1038/nature16482 ]
- Dna-rna synthetic construct (30 kDa, Macromolecule from unidentified)
- Dna-directed rna polymerase ii (520 kDa, Protein from Bos taurus)
- Dna-directed rna polymerase ii subunit grinl1a (40 kDa, Protein from Homo sapiens)
- Bovine pol ii elongation complex (590 kDa, Sample)
Structure of transcribing mammalian RNA polymerase II (EC3)
Bernecky C, Herzog F, Baumeister W, Plitzko JM, Cramer P
Nature (2016) 529 pp. 551-554 [ Pubmed: 26789250 DOI: doi:10.1038/nature16482 ]
- Dna-rna synthetic construct (30 kDa, Macromolecule from unidentified)
- Dna-directed rna polymerase ii (520 kDa, Protein from Bos taurus)
- Dna-directed rna polymerase ii subunit grinl1a (40 kDa, Protein from Homo sapiens)
- Bovine pol ii elongation complex (590 kDa, Sample)
Structure of transcribing mammalian RNA polymerase II (EC1)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5flm
Q-score: 0.442
Bernecky C, Herzog F, Baumeister W, Plitzko JM, Cramer P
Nature (2016) 529 pp. 551-554 [ Pubmed: 26789250 DOI: doi:10.1038/nature16482 ]
- Dna-rna synthetic construct (30 kDa, Macromolecule from unidentified)
- Dna-directed rna polymerase ii (520 kDa, Protein from Bos taurus)
- Dna-directed rna polymerase ii subunit grinl1a (40 kDa, Protein from Homo sapiens)
- Bovine pol ii elongation complex (590 kDa, Sample)