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Inactivate state of Maribacter polysiphoniae Argonuate (short pAgo system)
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8fex
Q-score: 0.462
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
- Tir-apaz (53 kDa, Protein from Maribacter polysiphoniae)
- Short pago (58 kDa, Protein from Maribacter polysiphoniae)
- Mapsparta heterodimeric complex(short pago) (Complex from Maribacter polysiphoniae)
Structure of tetramerized MapSPARTA upon guide RNA-mediated target DNA binding
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8ffi
Q-score: 0.566
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
- Guide rna (6 kDa, RNA from synthetic construct)
- Tir-apaz (53 kDa, Protein from Maribacter polysiphoniae)
- Short pago (58 kDa, Protein from Maribacter polysiphoniae)
- Target dna (7 kDa, DNA from synthetic construct)
- Structure of tetramerized mapsparta upon guide rna-mediated target dna binding (Complex from Maribacter polysiphoniae)
- Magnesium ion (24 Da, Ligand)
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
Focused refined map of TIR domain for MapSPARTA
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
Focused map for corner area4 of MapSPARTA
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
Local refined map of corner area 3 for the MapSPARTA
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
Focused refinement of corner area2 for MapSPARTA
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
Focused refinement for corner area1 of MapSPARTA
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
Symmetric dimer of MapSPARTA bound with gRNA/tDNA hybrid
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 8sp0
Q-score: 0.33
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
- Guide rna (6 kDa, RNA from synthetic construct)
- Symmetric dimer of mapsparta bound with grna/tdna hybrid (Complex from Maribacter polysiphoniae)
- Short pago (58 kDa, Protein from Maribacter polysiphoniae)
- Target dna (7 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Tir-apaz (53 kDa, Protein from Maribacter polysiphoniae)
Asymmetric dimer of MapSPARTA bound with gRNA/tDNA hybrid
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 8sp3
Q-score: 0.298
Shen Z, Yang XY, Xia S, Huang W, Taylor DJ, Nakanishi K, Fu TM
Nature (2023) 621 pp. 154-161 [ DOI: doi:10.1038/s41586-023-06456-z Pubmed: 37494956 ]
- Asymmetric dimer of mapsparta bound with grna/tdna hybrid (Complex from Maribacter polysiphoniae)
- Guide rna (6 kDa, RNA from Maribacter polysiphoniae)
- Short pago (58 kDa, Protein from Maribacter polysiphoniae)
- Target dna (7 kDa, DNA from Maribacter polysiphoniae)
- Magnesium ion (24 Da, Ligand)
- Tir-apaz (53 kDa, Protein from Maribacter polysiphoniae)