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Cryo-EM structure of pre-60S ribosomal subunit, unmethylated G2922
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7ug6
Q-score: 0.533
Yelland JN, Bravo JPK, Black JJ, Taylor DW, Johnson AW
Nat Struct Mol Biol (2023) 30 pp. 91-98 [ Pubmed: 36536102 DOI: doi:10.1038/s41594-022-00891-8 ]
- Nucle(ol)ar intermediate of large ribosomal subunit biogenesis, immunopurified by nog2-3xflag (Complex from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l12-a (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- Bud site selection protein 20 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein rpf2 (39 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein alb1 (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae S288C)
- Regulator of ribosome biosynthesis (23 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nucleolar gtp-binding protein 2 (59 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Ribosome assembly protein 4 (56 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)